Recombinant
RabMAb

Recombinant Anti-Olig2 antibody [EPR2673] - BSA and Azide free (ab220796)

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Overview

  • Product name

    Anti-Olig2 antibody [EPR2673] - BSA and Azide free
    See all Olig2 primary antibodies

  • Description

    Rabbit monoclonal [EPR2673] to Olig2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, IHC-Frmore details
    Unsuitable for: ICC/IF

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide, corresponding to residues in human Olig2.

  • Positive control

    • Oligodendroglioma lysate, human glioma tissue

  • General notes

    Ab220796 is the carrier-free version of ab109186. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab220796 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab220796 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 32 kDa.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for ICC/IF.

    • Target

    • Function

      Required for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. Cooperates with OLIG1 to establish the pMN domain of the embryonic neural tube. Antagonist of V2 interneuron and of NKX2-2-induced V3 interneuron development.

    • Tissue specificity

      Expressed in the brain, in oligodendrocytes. Strongly expressed in oligodendrogliomas, while expression is weak to moderate in astrocytomas. Expression in glioblastomas highly variable.

    • Involvement in disease

      Note=A chromosomal aberration involving OLIG2 may be a cause of a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(14;21)(q11.2;q22) with TCRA.

    • Sequence similarities

      Contains 1 basic helix-loop-helix (bHLH) domain.

    • Domain

      The bHLH is essential for interaction with NKX2-2.

    • Cellular localization

      Nucleus. Cytoplasm. The NLS contained in the bHLH domain could be masked in the native form and translocation to the nucleus could be mediated by interaction either with class E bHLH partner protein or with NKX2-2.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • Basic domain helix loop helix protein class B 1 antibody
      • Basic helix loop helix protein class B 1 antibody
      • BHLHB antibody
      • bHLHB1 antibody
      • bHLHe19 antibody
      • Class B basic helix loop helix protein 1 antibody
      • Class B basic helix-loop-helix protein 1 antibody
      • class E basic helix loop helix protein 19 antibody
      • Class E basic helix-loop-helix protein 19 antibody
      • Human protein kinase C binding protein RACK17 antibody
      • Olig2 antibody
      • OLIG2_HUMAN antibody
      • Oligo2 antibody
      • Oligodendrocyte lineage transcription factor 2 antibody
      • Oligodendrocyte specific bHLH transcription factor 2 antibody
      • Oligodendrocyte transcription factor 2 antibody
      • OTTHUMP00000067569 antibody
      • OTTHUMP00000067570 antibody
      • PRKCBP2 antibody
      • Protein kinase C binding protein 2 antibody
      • Protein kinase C binding protein RACK17 antibody
      • Protein kinase C-binding protein 2 antibody
      • Protein kinase C-binding protein RACK17 antibody
      • RACK17 antibody
      see all

    Images

    • Other - Anti-Olig2 antibody [EPR2673] - BSA and Azide free (ab220796)
      Other - Anti-Olig2 antibody [EPR2673] - BSA and Azide free (ab220796)This image is courtesy of the Single Cell & Imaging Mass Cytometry Analysis Platform, Goodman Cancer Research Centre, McGill University

      Imaging Mass Cytometry™ (IMC™) image of human glioblastoma brain cancer tissue stained with Anti-Olig2 antibody [EPR2673]. ab220796 (carrier-free antibody, purified) was metal-conjugated using a Maxpar® Antibody Labeling Kit from Fluidigm. Immunostaining was performed according to Fluidigm’s protocols. Briefly, slides were subject to deparaffinization and heat-induced epitope retrieval, followed by overnight incubation at 4°C with an antibody cocktail containing metal-tagged antibodies in blocking buffer. Slides were subsequently washed with 0.2% Triton-X and 1x PBS, counterstained with Cell-ID™ Intercalator-Ir diluted at 1/400 in 1x PBS for 30 min at room temperature, rinsed for 5 min with distilled H2O, and air-dried prior to IMC™ acquisition. IMC™ acquisition was performed using the Fluidigm Hyperion™ Imaging System.

      Imaging Mass Cytometry™, IMC™, Cell-ID™, Hyperion™ and Maxpar® are trademarks of Fluidigm Canada

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Olig2 antibody [EPR2673] - BSA and Azide free (ab220796)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Olig2 antibody [EPR2673] - BSA and Azide free (ab220796)
      Immunohistochemical staining of paraffin embedded rat cerebral cortex with purified ab109186 at a working dilution of 1/100. The secondary antibody used is ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L), at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109186).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Olig2 antibody [EPR2673] - BSA and Azide free (ab220796)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Olig2 antibody [EPR2673] - BSA and Azide free (ab220796)
      Immunohistochemical staining of paraffin embedded human cerebral cortex with purified ab109186 at a working dilution of 1/100. The secondary antibody used is ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L), at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109186).

    • Immunohistochemistry (Frozen sections) - Anti-Olig2 antibody [EPR2673] - BSA and Azide free (ab220796)
      Immunohistochemistry (Frozen sections) - Anti-Olig2 antibody [EPR2673] - BSA and Azide free (ab220796)This image is courtesy of an Abreview submitted by Ruma Raha-Chowdhury.

      Unpurified ab109186 staining Olig2 in rat brain tissue sections by immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.3% Triton X-100 in 0.1M PBS and blocked with 10% serum for 1 hour at 24°C. Samples were incubated with primary antibody (1/200 in PBS + 10% donkey serum) for 4 hours at 24°C. An Alexa Fluor® 488-conjugated anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody. Nuclei stained with Hoechst (blue).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109186).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Olig2 antibody [EPR2673] - BSA and Azide free (ab220796)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Olig2 antibody [EPR2673] - BSA and Azide free (ab220796)

      Immunohistochemical staining of Olig2 in human glioma tissue with ab109186 at a dilution of 1/100.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109186).

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Other - Anti-Olig2 antibody [EPR2673] - BSA and Azide free (ab220796)
      Other - Anti-Olig2 antibody [EPR2673] - BSA and Azide free (ab220796)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109186).

    References

    This product has been referenced in:

    • Schmidt AF  et al. Intra-amniotic LPS causes acute neuroinflammation in preterm rhesus macaques. J Neuroinflammation 13:238 (2016). IHC-P ; Rhesus monkey . Read more (PubMed: 27596440) »
    • Raha AA  et al. Neuroprotective Effect of TREM-2 in Aging and Alzheimer's Disease Model. J Alzheimers Dis N/A:N/A (2016). IHC ; Mouse . Read more (PubMed: 27662313) »
    See all 3 Publications for this product

    Publishing research using ab220796? Please let us know so that we can cite the reference in this datasheet.

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