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  1. Link

    olig2-antibody-epr2673-bsa-and-azide-free-ab220796.pdf

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Neuroscience Cell Type Marker Glia marker Oligodendrocyte marker
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RecombinantRabMAb

Recombinant Anti-Olig2 antibody [EPR2673] - BSA and Azide free (ab220796)

  • Datasheet
  • Certificate of Compliance
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Western blot - Anti-Olig2 antibody [EPR2673] - BSA and Azide free (ab220796)
  • Mass Cytometry - Anti-Olig2 antibody [EPR2673] - BSA and Azide free (ab220796)
  • Immunocytochemistry/ Immunofluorescence - Anti-Olig2 antibody [EPR2673] - BSA and Azide free (ab220796)
  • Immunocytochemistry/ Immunofluorescence - Anti-Olig2 antibody [EPR2673] - BSA and Azide free (ab220796)
  • Immunocytochemistry/ Immunofluorescence - Anti-Olig2 antibody [EPR2673] - BSA and Azide free (ab220796)
  • Immunocytochemistry/ Immunofluorescence - Anti-Olig2 antibody [EPR2673] - BSA and Azide free (ab220796)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Olig2 antibody [EPR2673] - BSA and Azide free (ab220796)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Olig2 antibody [EPR2673] - BSA and Azide free (ab220796)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Olig2 antibody [EPR2673] - BSA and Azide free (ab220796)
  • OI-RD Scanning - Anti-Olig2 antibody [EPR2673] - BSA and Azide free (ab220796)
  • Anti-Olig2 antibody [EPR2673] - BSA and Azide free (ab220796)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2673] to Olig2 - BSA and Azide free
  • Suitable for: ICC/IF, WB, IHC-P, Mass Cytometry
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Unconjugated

You may also be interested in

Biochemical
Product image
Arachidonic acid, omega-6 Polyunsaturated fatty acid (ab120916)
Protein
Recombinant human Olig2 protein (ab169905)
Conjugation
Alexa Fluor® 594 Conjugation Kit (Fast) - Lightning-Link® (ab269822)

View more associated products

Overview

  • Product name

    Anti-Olig2 antibody [EPR2673] - BSA and Azide free
    See all Olig2 primary antibodies
  • Description

    Rabbit monoclonal [EPR2673] to Olig2 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-P, Mass Cytometrymore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Oligodendroglioma lysate, human glioma tissue. IMC: Human glioblastoma brain cancer tissue; ICC/IF: Rat primary glia cells. Primary mouse neurons/glia, DIV14 cells. IHC-P: Rat and human cerebral cortex tissue; Human glioma tissue.
  • General notes

    ab220796 is the carrier-free version of ab109186.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Dissociation constant (KD)

    KD = 1.50 x 10 -11 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR2673
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Cell Type Marker
    • Glia marker
    • Oligodendrocyte marker
    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors
    • Stem Cells
    • Neural Stem Cells
    • Neuron Restricted Lineage
    • Neuroscience
    • Development

Associated products

  • Alternative Versions

    • Anti-Olig2 antibody [EPR2673] (ab109186)
    • Alexa Fluor® 488 Anti-Olig2 antibody [EPR2673] (ab225099)
    • Alexa Fluor® 647 Anti-Olig2 antibody [EPR2673] (ab225100)
    • Alexa Fluor® 594 Anti-Olig2 antibody [EPR2673] (ab300729)
    • Alexa Fluor® 555 Anti-Olig2 antibody [EPR2673] (ab300730)
    • Alexa Fluor® 568 Anti-Olig2 antibody [EPR2673] (ab302808)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant human Olig2 protein (ab169905)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab220796 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 32 kDa.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Mass Cytometry
Use at an assay dependent concentration.
Notes
ICC/IF
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 32 kDa.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Mass Cytometry
Use at an assay dependent concentration.

Target

  • Function

    Required for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. Cooperates with OLIG1 to establish the pMN domain of the embryonic neural tube. Antagonist of V2 interneuron and of NKX2-2-induced V3 interneuron development.
  • Tissue specificity

    Expressed in the brain, in oligodendrocytes. Strongly expressed in oligodendrogliomas, while expression is weak to moderate in astrocytomas. Expression in glioblastomas highly variable.
  • Involvement in disease

    Note=A chromosomal aberration involving OLIG2 may be a cause of a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(14;21)(q11.2;q22) with TCRA.
  • Sequence similarities

    Contains 1 basic helix-loop-helix (bHLH) domain.
  • Domain

    The bHLH is essential for interaction with NKX2-2.
  • Cellular localization

    Nucleus. Cytoplasm. The NLS contained in the bHLH domain could be masked in the native form and translocation to the nucleus could be mediated by interaction either with class E bHLH partner protein or with NKX2-2.
  • Target information above from: UniProt accession Q13516 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 10215 Human
    • Entrez Gene: 50913 Mouse
    • Entrez Gene: 304103 Rat
    • Omim: 606386 Human
    • SwissProt: Q13516 Human
    • SwissProt: Q9EQW6 Mouse
    • Unigene: 176977 Human
    • Unigene: 37289 Mouse
    • Alternative names

      • Basic domain helix loop helix protein class B 1 antibody
      • Basic helix loop helix protein class B 1 antibody
      • BHLHB antibody
      • bHLHB1 antibody
      • bHLHe19 antibody
      • Class B basic helix loop helix protein 1 antibody
      • Class B basic helix-loop-helix protein 1 antibody
      • class E basic helix loop helix protein 19 antibody
      • Class E basic helix-loop-helix protein 19 antibody
      • Human protein kinase C binding protein RACK17 antibody
      • Olig2 antibody
      • OLIG2_HUMAN antibody
      • Oligo2 antibody
      • Oligodendrocyte lineage transcription factor 2 antibody
      • Oligodendrocyte specific bHLH transcription factor 2 antibody
      • Oligodendrocyte transcription factor 2 antibody
      • OTTHUMP00000067569 antibody
      • OTTHUMP00000067570 antibody
      • PRKCBP2 antibody
      • Protein kinase C binding protein 2 antibody
      • Protein kinase C binding protein RACK17 antibody
      • Protein kinase C-binding protein 2 antibody
      • Protein kinase C-binding protein RACK17 antibody
      • RACK17 antibody
      see all

    Images

    • Western blot - Anti-Olig2 antibody [EPR2673] - BSA and Azide free (ab220796)
      Western blot - Anti-Olig2 antibody [EPR2673] - BSA and Azide free (ab220796)
      This data was developed using ab109186, the same antibody clone in a different buffer formulation. Different batches of ab109186 were tested on Mouse brain lysate at 0.1 µg/ml. 15 µg of lysate was loaded in each lane. Bands observed at 32 kDa.
    • Mass Cytometry - Anti-Olig2 antibody [EPR2673] - BSA and Azide free (ab220796)
      Mass Cytometry - Anti-Olig2 antibody [EPR2673] - BSA and Azide free (ab220796)This image is courtesy of the Single Cell & Imaging Mass Cytometry Analysis Platform, Goodman Cancer Research Centre, McGill University

      Imaging Mass Cytometry™ (IMC™) image of human glioblastoma brain cancer tissue stained with Anti-Olig2 antibody [EPR2673]. ab220796 (carrier-free antibody, purified) was metal-conjugated using a Maxpar® Antibody Labeling Kit from Fluidigm. Immunostaining was performed according to Fluidigm’s protocols. Briefly, slides were subject to deparaffinization and heat-induced epitope retrieval, followed by overnight incubation at 4°C with an antibody cocktail containing metal-tagged antibodies in blocking buffer. Slides were subsequently washed with 0.2% Triton-X and 1x PBS, counterstained with Cell-ID™ Intercalator-Ir diluted at 1/400 in 1x PBS for 30 min at room temperature, rinsed for 5 min with distilled H2O, and air-dried prior to IMC™ acquisition. IMC™ acquisition was performed using the Fluidigm Hyperion™ Imaging System.

      Imaging Mass Cytometry™, IMC™, Cell-ID™, Hyperion™ and Maxpar® are trademarks of Fluidigm Canada

    • Immunocytochemistry/ Immunofluorescence - Anti-Olig2 antibody [EPR2673] - BSA and Azide free (ab220796)
      Immunocytochemistry/ Immunofluorescence - Anti-Olig2 antibody [EPR2673] - BSA and Azide free (ab220796)

      This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab109186).

      ab109186 staining Olig2 in primary mouse neurons/glia, DIV14 (prepared from E18 mouse hippocampal brain area, obtained from Transnetyx Tissue by BrainBits, LLC, cat.no. C57EHP) cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab109186 at 5µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

      Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.

    • Immunocytochemistry/ Immunofluorescence - Anti-Olig2 antibody [EPR2673] - BSA and Azide free (ab220796)
      Immunocytochemistry/ Immunofluorescence - Anti-Olig2 antibody [EPR2673] - BSA and Azide free (ab220796)

      This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab109186)

      ab109186 staining Olig2 in primary hippocampal rat neurons/glia, (obtained from Neuromics, cat. no. PC35101), DIV14. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab109186 at 1?g/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

      Also suitable in cells fixed with 100% methanol (5 min).

      Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

    • Immunocytochemistry/ Immunofluorescence - Anti-Olig2 antibody [EPR2673] - BSA and Azide free (ab220796)
      Immunocytochemistry/ Immunofluorescence - Anti-Olig2 antibody [EPR2673] - BSA and Azide free (ab220796)

      This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab109186).

      ab109186 staining Olig2 in primary mouse neurons/glia, DIV14 (prepared from E18 mouse hippocampal brain area, obtained from Transnetyx Tissue by BrainBits, LLC, cat.no. C57EHP) cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab109186 at 1µg/ml and ab53521, Mouse mono Anti-A2B5 antibody [105]. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab175702, Goat Anti-Mouse IgM mu chain (Alexa Fluor® 568) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

      Also suitable in cells fixed with 100% methanol (5 min).

      Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

    • Immunocytochemistry/ Immunofluorescence - Anti-Olig2 antibody [EPR2673] - BSA and Azide free (ab220796)
      Immunocytochemistry/ Immunofluorescence - Anti-Olig2 antibody [EPR2673] - BSA and Azide free (ab220796)

      This data was developed using ab109186, the same antibody clone in a different buffer formulation.

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary glia cell cells labelling Olig2 with ab109186 at 1/100 (1.23 µg/mL) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (2 µg/mL) (Green). Confocal image showing nuclear staining in rat primary glia cell. Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5 µg/mL) (Red). The Nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (2 µg/mL).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Olig2 antibody [EPR2673] - BSA and Azide free (ab220796)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Olig2 antibody [EPR2673] - BSA and Azide free (ab220796)
      Immunohistochemical staining of paraffin embedded rat cerebral cortex with purified ab109186 at a working dilution of 1/100. The secondary antibody used is ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L), at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109186).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Olig2 antibody [EPR2673] - BSA and Azide free (ab220796)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Olig2 antibody [EPR2673] - BSA and Azide free (ab220796)
      Immunohistochemical staining of paraffin embedded human cerebral cortex with purified ab109186 at a working dilution of 1/100. The secondary antibody used is ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L), at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109186).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Olig2 antibody [EPR2673] - BSA and Azide free (ab220796)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Olig2 antibody [EPR2673] - BSA and Azide free (ab220796)

      Immunohistochemical staining of Olig2 in human glioma tissue with ab109186 at a dilution of 1/100.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109186).

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • OI-RD Scanning - Anti-Olig2 antibody [EPR2673] - BSA and Azide free (ab220796)
      OI-RD Scanning - Anti-Olig2 antibody [EPR2673] - BSA and Azide free (ab220796)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109186).

    • Anti-Olig2 antibody [EPR2673] - BSA and Azide free (ab220796)
      Anti-Olig2 antibody [EPR2673] - BSA and Azide free (ab220796)

    Protocols

    • Immunohistochemistry protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (4)

    Publishing research using ab220796? Please let us know so that we can cite the reference in this datasheet.

    ab220796 has been referenced in 4 publications.

    • Schwabenland M  et al. Deep spatial profiling of human COVID-19 brains reveals neuroinflammation with distinct microanatomical microglia-T-cell interactions. Immunity 54:1594-1610.e11 (2021). PubMed: 34174183
    • Schmidt AF  et al. Intra-amniotic LPS causes acute neuroinflammation in preterm rhesus macaques. J Neuroinflammation 13:238 (2016). IHC-P ; Rhesus monkey . PubMed: 27596440
    • Raha AA  et al. Neuroprotective Effect of TREM-2 in Aging and Alzheimer's Disease Model. J Alzheimers Dis N/A:N/A (2016). IHC ; Mouse . PubMed: 27662313
    • Tan B  et al. Effects of FTY720 (Fingolimod) on Proliferation, Differentiation, and Migration of Brain-Derived Neural Stem Cells. Stem Cells Int 2016:9671732 (2016). WB ; Rat . PubMed: 27829841

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Immunohistochemistry (Frozen sections) abreview for Anti-Olig2 antibody [EPR2673] - BSA and Azide free

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (Glioblastoma multiforme)
    Permeabilization
    Yes - 0.25% TritonX-100
    Specification
    Glioblastoma multiforme
    Blocking step
    BSA as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 4%
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Apr 25 2022

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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