Anti-Olig2 (phospho S10 + S13 + S14) antibody (ab183487)
Key features and details
- Rabbit polyclonal to Olig2 (phospho S10 + S13 + S14)
- Suitable for: WB
- Reacts with: Rat
- Isotype: IgG
Overview
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Product name
Anti-Olig2 (phospho S10 + S13 + S14) antibody
See all Olig2 primary antibodies -
Description
Rabbit polyclonal to Olig2 (phospho S10 + S13 + S14) -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Rat
Predicted to work with: Mouse, Guinea pig, Human, Non human primates, Zebrafish
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Immunogen
Synthetic peptide within Human Olig2 (phospho S10 + S13 + S14) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
Database link: Q13516 -
Positive control
- Rat neonatal brain lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.50
Constituents: 0.24% HEPES, 0.88% Sodium chloride, 0.01% BSA, 50% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab183487 is prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab183487 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Predicted molecular weight: 32 kDa.
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| Notes |
|---|
|
WB
1/1000. Predicted molecular weight: 32 kDa. |
Target
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Function
Required for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. Cooperates with OLIG1 to establish the pMN domain of the embryonic neural tube. Antagonist of V2 interneuron and of NKX2-2-induced V3 interneuron development. -
Tissue specificity
Expressed in the brain, in oligodendrocytes. Strongly expressed in oligodendrogliomas, while expression is weak to moderate in astrocytomas. Expression in glioblastomas highly variable. -
Involvement in disease
Note=A chromosomal aberration involving OLIG2 may be a cause of a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(14;21)(q11.2;q22) with TCRA. -
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain. -
Domain
The bHLH is essential for interaction with NKX2-2. -
Cellular localization
Nucleus. Cytoplasm. The NLS contained in the bHLH domain could be masked in the native form and translocation to the nucleus could be mediated by interaction either with class E bHLH partner protein or with NKX2-2. - Information by UniProt
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Database links
- Entrez Gene: 100716627 Guinea pig
- Entrez Gene: 10215 Human
- Entrez Gene: 50913 Mouse
- Entrez Gene: 304103 Rat
- Entrez Gene: 325288 Zebrafish
- Omim: 606386 Human
- SwissProt: Q13516 Human
- SwissProt: Q9EQW6 Mouse
see all -
Alternative names
- Basic domain helix loop helix protein class B 1 antibody
- Basic helix loop helix protein class B 1 antibody
- BHLHB antibody
see all
Images
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Western blot - Anti-Olig2 (phospho S10 + S13 + S14) antibody (ab183487)All lanes : Anti-Olig2 (phospho S10 + S13 + S14) antibody (ab183487) at 1/1000 dilution
Lane 1 : Rat neonatal brain lysate
Lane 2 : Rat neonatal brain lysate with immunizing phosphopeptide
Predicted band size: 32 kDa
Datasheets and documents
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Datasheet download
References (2)
ab183487 has been referenced in 2 publications.
- Liu H et al. Olig2 SUMOylation protects against genotoxic damage response by antagonizing p53 gene targeting. Cell Death Differ N/A:N/A (2020). PubMed: 32483381
- Tsigelny IF et al. Multiple spatially related pharmacophores define small molecule inhibitors of OLIG2 in glioblastoma. Oncotarget 8:22370-22384 (2017). WB ; Human . PubMed: 26517684
