Overview

  • Product name

  • Description

    Mouse polyclonal to Olig3
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Chicken, Human
    Predicted to work with: Mouse
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 1-272 of Human Olig3 (NP_786923.1, UniProt ID: Q7RTU3)

  • Positive control

    • Lysate from 293T cells transfected with Olig3; HeLa cells; Human lymph node tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    pH: 7.20
    Constituent: 100% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab168573 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 29 kDa.
IHC-P Use a concentration of 3 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • Function

    May determine the distinct specification program of class A neurons in the dorsal part of the spinal cord and suppress specification of class B neurons.
  • Sequence similarities

    Contains 1 bHLH (basic helix-loop-helix) domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Basic helix loop helix domain containing class B protein 7 antibody
    • Basic helix-loop-helix domain-containing protein, class B, 7 antibody
    • bHLHB7 antibody
    • bHLHe20 antibody
    • Class B basic helix loop helix protein 7 antibody
    • Class B basic helix-loop-helix protein 7 antibody
    • Class E basic helix loop helix protein 20 antibody
    • Class E basic helix-loop-helix protein 20 antibody
    • Olig3 antibody
    • OLIG3_HUMAN antibody
    • Oligo3 antibody
    • Oligodendrocyte lineage transcription factor 3 antibody
    • Oligodendrocyte specific bHLH transcription factor 3 antibody
    • Oligodendrocyte transcription factor 3 antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed paraffin-embedded Human lymph node tissue, labeling Olig3 using purified ab157368 at 3 µg/ml.
  • All lanes : Anti-Olig3 antibody (ab168573) at 1 µg/ml

    Lane 1 : Lysate from 293T cells transfected with Olig3
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 15 µl per lane.

    Developed using the ECL technique.

    Predicted band size: 29 kDa

  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, labeling Olig3 using ab168573 at 10 µg/ml.

References

This product has been referenced in:

  • Sasai N  et al. Integration of signals along orthogonal axes of the vertebrate neural tube controls progenitor competence and increases cell diversity. PLoS Biol 12:e1001907 (2014). IHC-P, ICC/IF ; Chicken . Read more (PubMed: 25026549) »
See 1 Publication for this product

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