Key features and details
- Rabbit polyclonal to Olig3
- Suitable for: IHC-P, WB, Flow Cyt
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-Olig3 antibody
See all Olig3 primary antibodies
DescriptionRabbit polyclonal to Olig3
Tested applicationsSuitable for: IHC-P, WB, Flow Cytmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide within Human Olig3 aa 74-100 (internal sequence) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: Q7RTU3
- IHC-P: Human testis and skin tissues. WB: Mouse spleen lysate. Flow Cyt: U-251 MG cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab230626 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 29 kDa.|
|Flow Cyt||1/10 - 1/50.|
FunctionMay determine the distinct specification program of class A neurons in the dorsal part of the spinal cord and suppress specification of class B neurons.
Sequence similaritiesContains 1 bHLH (basic helix-loop-helix) domain.
- Information by UniProt
- Basic helix loop helix domain containing class B protein 7 antibody
- Basic helix-loop-helix domain-containing protein, class B, 7 antibody
- bHLHB7 antibody
Formalin-fixed, paraffin-embedded human testis tissue stained for Olig3 using ab230626 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human skin tissue stained for Olig3 using ab230626 at 1/100 dilution in immunohistochemical analysis.
Anti-Olig3 antibody (ab230626) at 1/1000 dilution + Mouse spleen lysate at 35 µg
Predicted band size: 29 kDa
Flow cytometric analysis of U-251 MG (human brain glioma cell line) cell line labeling Olig3 with ab230626 at 1/10 dilution (green) compared with a negative control cell (blue).
FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab230626 has not yet been referenced specifically in any publications.