Recombinant
RabMAb

Recombinant Anti-Olig3 antibody [EPR6511] (ab129197)

Overview

  • Product name

    Anti-Olig3 antibody [EPR6511]
    See all Olig3 primary antibodies
  • Description

    Rabbit monoclonal [EPR6511] to Olig3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Olig3 aa 250-350 (C terminal). The exact sequence is proprietary.

  • Positive control

    • Human fetal brain, U-87 MG, and 293T cell lysates; U-87 MG cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 1.70 x 10 -11 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR6511
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab129197 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 29 kDa.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • Function

      May determine the distinct specification program of class A neurons in the dorsal part of the spinal cord and suppress specification of class B neurons.
    • Sequence similarities

      Contains 1 bHLH (basic helix-loop-helix) domain.
    • Cellular localization

      Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • Basic helix loop helix domain containing class B protein 7 antibody
      • Basic helix-loop-helix domain-containing protein, class B, 7 antibody
      • bHLHB7 antibody
      • bHLHe20 antibody
      • Class B basic helix loop helix protein 7 antibody
      • Class B basic helix-loop-helix protein 7 antibody
      • Class E basic helix loop helix protein 20 antibody
      • Class E basic helix-loop-helix protein 20 antibody
      • Olig3 antibody
      • OLIG3_HUMAN antibody
      • Oligo3 antibody
      • Oligodendrocyte lineage transcription factor 3 antibody
      • Oligodendrocyte specific bHLH transcription factor 3 antibody
      • Oligodendrocyte transcription factor 3 antibody
      see all

    Images

    • ab129197, at 1/100 dilution, staining Olig3 in U-87 MG cells by Immunofluorescence.
    • All lanes : Anti-Olig3 antibody [EPR6511] (ab129197) at 1/1000 dilution

      Lane 1 : Human fetal brain lysate
      Lane 2 : U-87 MG lysate
      Lane 3 : 293T lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size: 29 kDa

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab129197 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (E12 fetal mouse brain)
    Permeabilization
    Yes - 0.2% Triton X100
    Specification
    E12 fetal mouse brain
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 24°C
    Fixative
    Formaldehyde

    Bastiaan Van Den Berg

    Verified customer

    Submitted Mar 29 2016

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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