Anti-Oligodendrocyte myelin glycoprotein antibody (ab96426)

Overview

  • Product name

    Anti-Oligodendrocyte myelin glycoprotein antibody
    See all Oligodendrocyte myelin glycoprotein primary antibodies
  • Description

    Rabbit polyclonal to Oligodendrocyte myelin glycoprotein
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Cow, Macaque monkey, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region within amino acids 249 - 311 of Human Oligodendrocyte myelin glycoprotein.

  • Positive control

    • A549, IMR32 or HCT116 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab96426 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 49 kDa.

Target

  • Function

    Cell adhesion molecule contributing to the interactive process required for myelination in the central nervous system.
  • Tissue specificity

    Oligodendrocytes and myelin of the central nervous system.
  • Sequence similarities

    Contains 8 LRR (leucine-rich) repeats.
    Contains 1 LRRNT domain.
  • Post-translational
    modifications

    O-glycosylated in its Ser/Thr-rich repeat domain.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Oligodendrocyte-myelin glycoprotein antibody
    • Omg antibody
    • OMGP antibody
    • OMGP_HUMAN antibody

Images

  • Anti-Oligodendrocyte myelin glycoprotein antibody (ab96426) at 1/1000 dilution + IMR32 whole cell lysate at 30 µg

    Predicted band size: 49 kDa



    10% SDS PAGE

References

ab96426 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Western blot
Sample
Human Cell lysate - whole cell (Glioma)
Gel Running Conditions
Reduced Denaturing (12% gel)
Loading amount
25 µg
Specification
Glioma
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Apr 27 2016

Answer

Thank you for contacting us.

As requested I have attached the datasheeet to this email. I do not have alot of addition data regarding additional bands at the higher molecular weight, however this protein does undergo post translational modifications which may change its weight affect charge and migration through the gel.

I would recommend heating your samples for a longer period in a reducing buffer to remove these modifications.

We do offer anotherAnti-Oligodendrocyte myelin glycoprotein antibody which we guarantee in Western blot . This product, ab78595, was created against the synthetic peptide: C-KVTKIPKQYRTKE, corresponding to internal sequence amino acids 299-311 of Human Oligodendrocyte myelin glycoprotein according to NP_002535.3

https://www.abcam.com/Oligodendrocyte-myelin-glycoprotein-antibody-ab78595.html

While we know this to work in WB against this protein and have predicted it to work in a number of species,Cow, Human, Cynomolgus Monkey, Orangutan, we have not yet tested in each of these.For UNTESTED species, we have established a testing discount program. Here is a brief description of how it works:

The testing discount program is for customers who like to use an antibody/protein on an untested species/application. You would purchase the antibody at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount for ONE antibody at the full price (100%) of the antibody you have tested. The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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