Western blot abreview for Anti-Oligodendrocyte myelin glycoprotein antibody

Good
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (Glioma)
Gel Running Conditions
Reduced Denaturing (12% gel)
Loading amount
25 µg
Specification
Glioma
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
15 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Milk 1% in TBS Tween 0.1%
Dilution
1/1000

Secondary antibody

Dilution
1/10000
Name
Non-Abcam antibody was used: Bio-Rad Goat Anti-rabbit IgG (H+L)
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: 40 kDa Non-specific: 23, 20 kDa

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Submitted Apr 27 2016

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