Overview

  • Product name
    Anti-Oligodendrocyte Specific Protein antibody
    See all Oligodendrocyte Specific Protein primary antibodies
  • Description
    Rabbit polyclonal to Oligodendrocyte Specific Protein
  • Host species
    Rabbit
  • Specificity
    Detects endogenous levels of total Oligodendrocyte Specific Protein
  • Tested applications
    Suitable for: IHC-Fr, ICC/IF, WB, ELISA, IHC-P, IHC (PFA fixed)more details
  • Species reactivity
    Reacts with: Mouse, Rat, Sheep, Goat, Chicken, Cow, Cat, Human, Common marmoset
  • Immunogen

    Synthetic peptide (Human)

  • Positive control
    • WB: Mouse brain extract. ICC/IF: Rat cortical neurons. Mouse mixed glia cells. IHC-P: Cat and mouse brain tissue. IHC-Fr: Rat brain tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab53041 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
ICC/IF Use a concentration of 1 µg/ml.
WB 1/500 - 1/1000. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).
ELISA 1/5000.
IHC-P 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC (PFA fixed) 1/3000.

Target

Images

  • All lanes : Anti-Oligodendrocyte Specific Protein antibody (ab53041) at 1/500 dilution

    Lane 1 : Mouse brain extract
    Lane 2 : Mouse brain extract with peptide

    Predicted band size: 22 kDa
    Observed band size: 22 kDa



    Secondary antibody - anti-rabbit HRP (ab6721)

  • IF image of ab53041 staining cryopreserved rat cortical neurons grown for 20 to 29 DIV.

    Right panel showing a non treated culture (Control).

    Left panel showing cultures treated with Triodothyronine (T3), 60ng/mL, starting at 10 DIV.

    Cells were fixed with 4% paraformaldehyde containing 0.2% picric acid in 0.1M phosphate buffer, pH 6.9 for 20 minutes.

    The cells were then incubated at 4°C over night with ab53041 (1:200) diluted in PBS containing 0.3% Triton-X 100 (PBSTx).

    The secondary antibody used was ab150068 (1:200).

  • ab53041 staining cat brain sections by IHC-P. The tissue was fixed with formaldehyde and a heat mediated antigen retrival step was performed with citric acid pH 6. Blocking of the sample was done with 1% BSA for 10 minutes at 21°C, followed by staining with ab53041 at 1/500 in TRIS/BSA/azide for 2h at 21°C. A biotinylated goat anti-rabbit polyclonal antibody was used as the secondary antibody.

    See Abreview

  • ab53041 staining Oligodendrocyte Specific Protein in mixed glia prepared from mouse brain by Immunocytochemistry/ Immunofluorescence. The cells were fixed in ice-cold methanol and then blocked using 20% serum with 0.5% Saponin for 1 hour at 4°C. Samples were then incubated with primary antibody at 1/200 for 2 hours at 23°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to Alexa Fluor® 488 (green) used at a 1/400 dilution. DAPI was used to stain the cell nuclei (blue).

    See Abreview

  • ab53041 diluted 1/500 in 2% normal goat serum staining rat brain tissue sections by IHC-Fr. The tissue was formaldehyde fixed and blocked with 2% serum for 30 minutes at 21°C before incubation with the primary antibody for 24 hours at 21°C.  A biotinylated goat anti-rabbit antibody was used as the secondary.

    See Abreview

  • ab53041 staining Oligodendrocyte Specific Protein in mouse brain tissue section by Immunohistochemistry (PFA-fixed). The tissue sections were fixed with 4% paraformaldehyde.The primary antibody was diluted 1/3000 and incubated with sample for 16 hours at 4°C in PBS 0.3% Triton 8% BSA. An Alexa Fluor® 568 conjugated goat polyclonal to rabbit IgG was used at dilution 1/1000 as secondary antibody. The image was taken from the cortex of an adult mouse, near the midline.

    See Abreview

References

This product has been referenced in:
  • Li HP  et al. Inactivation of the tight junction gene CLDN11 by aberrant hypermethylation modulates tubulins polymerization and promotes cell migration in nasopharyngeal carcinoma. J Exp Clin Cancer Res 37:102 (2018). WB . Read more (PubMed: 29747653) »
  • Hollenbach J  et al. Loss of connexin43 in murine Sertoli cells and its effect on blood-testis barrier formation and dynamics. PLoS One 13:e0198100 (2018). Read more (PubMed: 29856785) »
See all 35 Publications for this product

Customer reviews and Q&As

1-10 of 21 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (cortical glia)
Permeabilization
Yes - 0.1% tritonX
Specification
cortical glia
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted May 04 2018

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Chicken Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Brain
Blocking step
BSA as blocking agent for 1 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde

Mr. Carl Hobbs

Verified customer

Submitted May 24 2016

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (In vitro differentiated mouse neural stem cells)
Permeabilization
No
Specification
In vitro differentiated mouse neural stem cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative
Methanol

Abcam user community

Verified customer

Submitted Apr 30 2015

Application
Immunocytochemistry
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Sample
Human Cultured Cells (engineered nervous tissue)
Specification
engineered nervous tissue
Permeabilization
No
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Jan 20 2015

Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing (10% separating gel)
Sample
Mouse Tissue lysate - whole (Hippocampal homogenate)
Specification
Hippocampal homogenate
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Apr 01 2014

Application
IHC - Wholemount
Sample
Zebrafish Embryo (48hpf oligodendrocyte/motoneurons)
Specification
48hpf oligodendrocyte/motoneurons

Abcam user community

Verified customer

Submitted Mar 05 2014

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Sample
Lizard Tissue sections (Spinal cord)
Specification
Spinal cord
Permeabilization
No
Fixative
Formaldehyde

Mr. Carl Hobbs

Verified customer

Submitted Jun 07 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (testis)
Specification
testis
Fixative
bouin's solution
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 1.5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Apr 30 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (mouse brain (cortex))
Specification
mouse brain (cortex)
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
Yes - Triton during block and staining
Blocking step
PBS, 10%Horse serum, 0,2%BSA, 0,3%Triton as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Feb 08 2013

Answer

Thank you for contacting us.

Could you please clarify which publication you are referring to? We have four different references listed on the datasheet for ab53041.

While the O4 clone is a very well known oligodendrocyte marker, it is no longer being produced by our lab. The oligodendrocyte specific protein antibody ab53041 is guaranteed for use as an oligodendrocyte marker in the applications and species listed on our datasheet. If you have any trouble using this antibody, please let us know within six months of purchase and we will be happy to offer you a refund or replacement. I hope this helps, please let me know if you need any additional information or assistance.

Read More

1-10 of 21 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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