Recombinant Anti-Oligodendrocyte Specific Protein antibody [EPR12726] (ab175236)
![Western blot - Anti-Oligodendrocyte Specific Protein antibody [EPR12726] (ab175236)](https://www.abcam.com/ps/products/175/ab175236/Images/ab175236-201637-anti-oligodendrocyte-specific-protein-antibody-epr12726-western-blot.jpg "Western blot - Anti-Oligodendrocyte Specific Protein antibody [EPR12726] (ab175236)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12726] to Oligodendrocyte Specific Protein
- Suitable for: WB, Flow Cyt
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Oligodendrocyte Specific Protein antibody [EPR12726]
See all Oligodendrocyte Specific Protein primary antibodies -
Description
Rabbit monoclonal [EPR12726] to Oligodendrocyte Specific Protein -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cytmore details
Unsuitable for: ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Cynomolgus monkey, Orangutan
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Immunogen
Synthetic peptide within Human Oligodendrocyte Specific Protein aa 1-100 (Cysteine residue). The exact sequence is proprietary.
Database link: O75508 -
Positive control
- Human oligodendroglioma, U87-MG, SH-SY5Y, fetal brain and Human testis lysates; U87-MG cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR12726 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab175236 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 22 kDa.
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| Flow Cyt |
1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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| Notes |
|---|
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WB
1/1000 - 1/10000. Predicted molecular weight: 22 kDa. |
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Flow Cyt
1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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Target
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Function
Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity. -
Sequence similarities
Belongs to the claudin family. -
Cellular localization
Cell junction > tight junction. Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 508268. Cow
- Entrez Gene: 5010 Human
- Entrez Gene: 18417 Mouse
- Entrez Gene: 84588 Rat
- Omim: 601326 Human
- SwissProt: Q3MHK4 Cow
- SwissProt: Q4R3L1 Cynomolgus monkey
- SwissProt: O75508 Human
see all -
Alternative names
- Claudin 11 antibody
- Claudin-11 antibody
- CLD11_HUMAN antibody
see all
Images
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Western blot - Anti-Oligodendrocyte Specific Protein antibody [EPR12726] (ab175236)All lanes : Anti-Oligodendrocyte Specific Protein antibody [EPR12726] (ab175236) at 1/1000 dilution
Lane 1 : Human oligodendroglioma lysate
Lane 2 : U87-MG lysate
Lane 3 : SH-SY5Y lysate
Lane 4 : Fetal brain lysate
Lane 5 : Human testis lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 22 kDa -
![Flow Cytometry - Anti-Oligodendrocyte Specific Protein antibody [EPR12726] (ab175236)](https://www.abcam.com/ps/products/175/ab175236/Images/ab175236-201636-anti-oligodendrocyte-specific-protein-antibody-epr12726-flow-cytometry.jpg)
Flow Cytometry - Anti-Oligodendrocyte Specific Protein antibody [EPR12726] (ab175236)Flow Cytometrical analysis of U87-MG cells labeling Oligodendrocyte Specific Protein with ab175236 antibody at a dilution of 1/100 (red) or a rabbit IgG (negative) (green).
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![Anti-Oligodendrocyte Specific Protein antibody [EPR12726] (ab175236)](https://www.abcam.com/ps/products/175/ab175236/Images/ab175236-3-benefits-of-recombinant-antibodies.png)
Anti-Oligodendrocyte Specific Protein antibody [EPR12726] (ab175236)
Datasheets and documents
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Datasheet download
References (2)
ab175236 has been referenced in 2 publications.
- Wang J et al. Androgen promotes differentiation of PLZF+ spermatogonia pool via indirect regulatory pattern. Cell Commun Signal 17:57 (2019). PubMed: 31142324
- Wang B et al. Oridonin overcomes the gemcitabine resistant PANC-1/Gem cells by regulating GST pi and LRP/1 ERK/JNK signalling. Onco Targets Ther 12:5751-5765 (2019). PubMed: 31410021
