Anti-Oligodendrocyte Specific Protein antibody - Oligodendrocyte Marker (ab7474)
Key features and details
- Rabbit polyclonal to Oligodendrocyte Specific Protein - Oligodendrocyte Marker
- Suitable for: IHC-P, IHC-Fr
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Oligodendrocyte Specific Protein antibody - Oligodendrocyte Marker
See all Oligodendrocyte Specific Protein primary antibodies -
Description
Rabbit polyclonal to Oligodendrocyte Specific Protein - Oligodendrocyte Marker -
Host species
Rabbit -
Specificity
Reacts specifically with 22 kDa protein derived from CNS samples. -
Tested applications
Suitable for: IHC-P, IHC-Frmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
A 15 residue synthetic C-terminal peptide.
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General notes
The concentration depends on the batch, please enquire for current stock concentration.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: Whole serum -
Concentration information loading...
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Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab7474 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | (2) |
1/50 - 1/500.
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IHC-Fr | (1) |
Use at an assay dependent concentration.
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Notes |
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IHC-P
1/50 - 1/500. |
IHC-Fr
Use at an assay dependent concentration. |
Target
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Function
Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity. -
Sequence similarities
Belongs to the claudin family. -
Cellular localization
Cell junction > tight junction. Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 5010 Human
- Entrez Gene: 84588 Rat
- Omim: 601326 Human
- SwissProt: O75508 Human
- SwissProt: Q99P82 Rat
- Unigene: 31595 Human
- Unigene: 8282 Rat
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Alternative names
- Claudin 11 antibody
- Claudin-11 antibody
- CLD11_HUMAN antibody
see all
Images
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Immunohistochemical analysis of adult mouse testis tissue, staining Oligodendrocyte Specific Protein (red) with ab7474.
Tissue was blocked with 5% BSA for 1 hour at 25°C. Samples were incubated with primary antibody (1/100 in 1% BSA) for 1 hour at 25°C. A TRITC-conjugated donkey anti-rabbit polyclonal IgG (1/200) was used as the secondary antibody. -
Immunofluorescent staining for OSP obtained with OSP antibody (ab7474) in rat brain cortex. The picture shows oligodendrocyte immunostaining in the cingular cortex. Picture taken with X20 objective, cells stained are ~30microns in diameter. Animals were intracardially perfused with 4% PFA. Tissue was post-fixed overnight in the same fixative, cryoprotected in 20% sucrose and frozen in OCT. Protocol : free floating IHC on 30um cryostat sections. Primary antibody ab7474 was used at 1/100 and incubated overnight at RT. Secondary antibody, Alexa fluor 488 at 1/1000, incubated for 2h at RT.
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IHC image of ab7474 staining in human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab7474, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Datasheets and documents
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Datasheet download
References (14)
ab7474 has been referenced in 14 publications.
- Valdivia AO & Bhattacharya SK Lyso-Lipid-Induced Oligodendrocyte Maturation Underlies Restoration of Optic Nerve Function. eNeuro 9:N/A (2022). PubMed: 35027445
- Wellman SM et al. Revealing Spatial and Temporal Patterns of Cell Death, Glial Proliferation, and Blood-Brain Barrier Dysfunction Around Implanted Intracortical Neural Interfaces. Front Neurosci 13:493 (2019). PubMed: 31191216
- Raynald et al. Polypyrrole/polylactic acid nanofibrous scaffold cotransplanted with bone marrow stromal cells promotes the functional recovery of spinal cord injury in rats. CNS Neurosci Ther 25:951-964 (2019). PubMed: 31486601
- Pan J et al. Expression of claudin-11 in a rat model of varicocele and its effects on the blood-testis barrier. Mol Med Rep 18:5647-5651 (2018). PubMed: 30365105
- Zhao X et al. Neutrophil polarization by IL-27 as a therapeutic target for intracerebral hemorrhage. Nat Commun 8:602 (2017). PubMed: 28928459