Overview

  • Product name

    Oligonucleotide Conjugation Kit
  • Assay time

    2h 00m
  • Product overview

    The Oligonucleotide Conjugation Kit (ab218260) enables the easy and efficient generation of antibody-oligonucleotide conjugates in less than 2 hours. The kit can be used to generate conjugates of different ratios of antibody: oligonucleotide. It also includes positive controls that allow the user to confirm that the conjugation chemistry has worked correctly.


    This kit can be used to conjugate antibodies to single-stranded oligonucleotides that are 10-120 bases long or to double-stranded oligonucleotides that are up to 80 bases long. The oligonucleotide should be synthesized to include a terminal amine group.


    The 1 test kit includes reagents for 1 conjugation of 100 µg of antibody and 1 control.


    The 3 test kit includes reagents for 3 conjugations of 100 µg of antibody and 1 control.


    As expected for any chemical conjugation, the concentration and buffer formulation of the oligonucleotide and the antibody need to fall within certain parameters, as detailed in the protocol booklet.

  • Tested applications

    Suitable for: Conjugationmore details

Properties

  • Storage instructions

    Please refer to protocols.
  • Components 1 tests 3 tests
    Antibody Activation Reagent 2 vials 4 vials
    Antibody Suspension Buffer 1 vial 1 vial
    Conjugate Clean Up Reagent 1 unit 1 unit
    Antibody Control 1 vial 1 vial
    Oligonucleotide Control 1 vial 1 vial
    Oligonucleotide Activation Reagent 2 vials 4 vials
    Separating Columns 4 units 8 units
    Wash Buffer 1 x 80ml 1 x 160ml

Applications

Our Abpromise guarantee covers the use of ab218260 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Conjugation Use at an assay dependent concentration.

Images

  • Oligonucleotide Conjugation Kit (ab218260) Reducing SDS-PAGE after Oligonucleotide Conjugation

Protocols

References

ab218260 has not yet been referenced specifically in any publications.

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