Product nameOligonucleotide Conjugation Kit
Assay time2h 00m
This product is manufactured by Expedeon, an Abcam company. Expedeon product code 425-0300 was previously called Thunder-Link® Plus Oligo Conjugation Kit 3 reaction control and is the same as the 3 tests size of this product. Expedeon product code 425-0000 was previously called Thunder-Link® Plus Oligo Conjugation Kit 1 reaction control and is the same as the 1 test size of this product.
The Oligonucleotide Conjugation Kit (ab218260) enables the easy and efficient generation of antibody-oligonucleotide conjugates in less than 2 hours. The kit can be used to generate conjugates of different ratios of antibody: oligonucleotide. It also includes positive controls that allow the user to confirm that the conjugation chemistry has worked correctly.
This kit can be used to conjugate antibodies to single-stranded oligonucleotides that are 10-120 bases long or to double-stranded oligonucleotides that are up to 80 bases long. The oligonucleotide should be synthesized to include a terminal amine group.
The 1 test kit includes reagents for 1 conjugation of 100 μg of antibody and 1 control.
The 3 test kit includes reagents for 3 conjugations of 100 μg of antibody and 1 control.
As expected for any chemical conjugation, the concentration and buffer formulation of the oligonucleotide and the antibody need to fall within certain parameters, as detailed in the protocol booklet.
Tested applicationsSuitable for: Conjugationmore details
Storage instructionsPlease refer to protocols.
Components 1 tests 3 tests Antibody Activation Reagent 2 vials 4 vials Antibody Suspension Buffer 1 vial 1 vial Conjugate Clean Up Reagent 1 unit 1 unit Antibody Control 1 vial 1 vial Oligonucleotide Control 1 vial 1 vial Oligonucleotide Activation Reagent 2 vials 4 vials Separating Columns 4 units 8 units Wash Buffer 1 x 80ml 1 x 160ml
Our Abpromise guarantee covers the use of ab218260 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Conjugation||Use at an assay dependent concentration.|
ab218260 has not yet been referenced specifically in any publications.