Overview

  • Product name

  • Description

    Rabbit polyclonal to OMA1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Sheep, Rabbit, Cow, Pig, Chimpanzee, Macaque monkey, Gorilla, Chinese hamster, Orangutan
  • Immunogen

    Synthetic peptide within Human OMA1 aa 400-500 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Database link: Q96E52

  • Positive control

    • This antibody gave a positive signal in Human, Mouse and Rat Heart tissue lysates. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal kidney.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab154949 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 60 kDa).
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Mitochondrial protease.
  • Tissue specificity

    Widely expressed, with strong expression in the heart, skeletal muscle, kidney and liver.
  • Sequence similarities

    Belongs to the peptidase M48 family.
  • Cellular localization

    Mitochondrion membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • 2010001O09Rik antibody
    • DAB1 antibody
    • FLJ33782 antibody
    • Metalloendopeptidase OMA1 antibody
    • Metalloendopeptidase OMA1, mitochondrial antibody
    • Metalloprotease-related protein 1 antibody
    • mitochondrial antibody
    • MPRP 1 antibody
    • MPRP-1 antibody
    • MPRP1 antibody
    • OMA1 antibody
    • OMA1 homolog zinc metallopeptidase antibody
    • OMA1 homolog, zinc metallopeptidase (S. cerevisiae) antibody
    • OMA1 zinc metallopeptidase antibody
    • OMA1 zinc metallopeptidase homolog antibody
    • OMA1_HUMAN antibody
    • Overlapping activity with M-AAA protease antibody
    • Overlapping with the m-AAA protease 1 homolog antibody
    • peptidase antibody
    • YKR087C antibody
    • Zinc metallopeptidase OMA1 antibody
    • ZMPOMA1 antibody
    see all

Images

  • IHC image of OMA1 staining in human normal kidney formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab154949, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • All lanes : Anti-OMA1 antibody (ab154949) at 1 µg/ml

    Lane 1 : Human heart tissue lysate - total protein (ab29431)
    Lane 2 : Heart (Mouse) Tissue Lysate
    Lane 3 : Heart (Rat) Tissue Lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 60 kDa
    Observed band size: 58 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 82 kDa (possible non-specific binding)


    Exposure time: 90 seconds


    The band observed at 58 kDa could potentially be a cleaved form of OMA1 due to the presence of a 13 amino acid transit peptide.

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab154949 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406

References

This product has been referenced in:

  • Axelrod CL  et al. Exercise training remodels human skeletal muscle mitochondrial fission and fusion machinery towards a pro-elongation phenotype. Acta Physiol (Oxf) N/A:e13216 (2018). Read more (PubMed: 30408342) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Western blot
Sample
Rat Tissue lysate - whole (Heart)
Gel Running Conditions
Reduced Denaturing
Loading amount
25 µg
Specification
Heart
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Jan 30 2019

Application
Western blot
Sample
Mouse Purified protein (Isolated mitochondrial lysate(cardiomyocyte))
Gel Running Conditions
Reduced Denaturing
Loading amount
25 µg
Specification
Isolated mitochondrial lysate(cardiomyocyte)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Jan 19 2019

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up