Overview

  • Product name

    Omalizumab ELISA Kit (Xolair®)
    See all Omalizumab kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall < 15%
    Inter-assay
    Sample n Mean SD CV%
    Overall < 15%
  • Sample type

    Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    10 ng/ml
  • Range

    10 ng/ml - 1000 ng/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 85% - 115%

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Omalizumab ELISA Kit (Xolair®) (ab237650) has been developed for specific quantification of Omalizumab concentration in human serum or plasma with high sensitivity and reproducibility. The density of color is proportional to the amount of Omalizumab captured from the samples and can be quantified when compared with standard curve.


    Omalizumab (Xolair®) is a recombinant DNA-derived humanized IgG1k monoclonal antibody that selectively binds to human immunoglobulin E (IgE). Xolair inhibits the binding of IgE to the high-affinity IgE receptor (FceRI) on the surface of mast cells and basophils. Reduction in surface-bound IgE on FceRI-bearing cells limits the degree of release of mediators of the allergic response. Xolair is used to treat severe, persistent asthma.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 96 tests
    Micro ELISA Plate 1 unit
    Omalizumab Standard S1 1 x 1ml
    Omalizumab Standard S2 1 x 1ml
    Omalizumab Standard S3 1 x 1ml
    Omalizumab Standard S4 1 x 1ml
    Omalizumab Standard S5 1 x 1ml
    Omalizumab Standard S6 1 x 1ml
    Omalizumab Standard S7 1 x 1ml
    Omalizumab Standard S8 1 x 1ml
    Assay Buffer 1 x 50ml
    HRP-conjugate Probe 1 x 12ml
    TMB substrate 1 x 12ml
    Stop Solution 1 x 12ml
    Wash buffer (20X) 1 x 50ml
    Plate sealers 2 units
  • Relevance

    Omalizumab (Xolair®) is a recombinant DNA-derived humanized IgG1k monoclonal antibody that selectively binds to human immunoglobulin E (IgE). Xolair inhibits the binding of IgE to the high-affinity IgE receptor (FceRI) on the surface of mast cells and basophils. Reduction in surface-bound IgE on FceRI-bearing cells limits the degree of release of mediators of the allergic response. Xolair is used to treat severe, persistent asthma.
  • Alternative names

    • Xolair

Applications

Our Abpromise guarantee covers the use of ab237650 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Typical Standard Curve: This standard curve is for demonstration only. A standard curve must be run with each assay.

Protocols

References

ab237650 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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