Overview

  • Product name

    Anti-ompF antibody
  • Description

    Rabbit polyclonal to ompF
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Bacteria
  • Immunogen

    Synthetic peptide within ompF aa 160-210 conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary. Yersinia enterocolitica subsp. enterocolitica 8081
    Sequence:

    NFFGLVDGLNF ALQYQGKNED GRNGVVETTD ALGNVTSTTY SNLKDQNGDG


    Database link: A1JMN7

  • Positive control

    • Human colon carcinoma tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 0.01% BSA, 50% Glycerol

    Aqueous buffered solution
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab203223 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/1000. Predicted molecular weight: 42 kDa.
IHC-P 1/100 - 1/500.

When using a fluorescent probe the recommended dilution is 1/50 – 1/200

Target

  • Cellular localization

    Cell Membrane
  • Alternative names

    • orpF antibody
    • Putative outer membrane porin F protein antibody
    • YE1563 antibody

Images

  • Immunohistochemical analysis of formalin-fixed and paraffin embedded Human colon carcinoma labeling ompF with ab203223 at 1/200 dilution, followed by Goat Anti-Rabbit IgG, Cy3 conjugated secondary antibody at 1/200 dilution.

  • Immunohistochemical analysis of formalin-fixed and paraffin embedded Human colon carcinoma labeling ompF with ab203223 at 1/200 dilution with DAB staining.

References

ab203223 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Escherichia coli Cell lysate - whole cell (E. coli strain BL21(DE3))
Gel Running Conditions
Reduced Denaturing (Invitrogen NuPAGE gel, 4-12% Bis-Tris (#NP0329BOX) in 1X MOPS-SDS (#NP0001)))
Loading amount
3.1 µg
Specification
E. coli strain BL21(DE3)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Oct 19 2018

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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