Overview

  • Product name
    Anti-Oncostatin M/OSM antibody
    See all Oncostatin M/OSM primary antibodies
  • Description
    Rabbit polyclonal to Oncostatin M/OSM
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ELISA, Neutralisingmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Highly pure (>98%) recombinant hOncostatin-M (human Oncostatin-M).

  • Positive control
    • Recombinant human Oncostatin M/OSM protein (ab9634) can be used as a positive control in WB.

Properties

Applications

Our Abpromise guarantee covers the use of ab9633 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
Neutralising
  • Application notes
    Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hOncostatin-M (10 ng/ml), a concentration of 0.1 - 0.175 µg/ml of this antibody is required

    ELISA:To detect hOncostatin-M by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hOncostatin-M.

    Western Blot:To detect hOncostatin-M by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hOncostatin-M is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
  • Target

    • Function
      Growth regulator. Inhibits the proliferation of a number of tumor cell lines. Stimulates proliferation of AIDS-KS cells. It regulates cytokine production, including IL-6, G-CSF and GM-CSF from endothelial cells. Uses both type I OSM receptor (heterodimers composed of LIPR and IL6ST) and type II OSM receptor (heterodimers composed of OSMR and IL6ST).
    • Sequence similarities
      Belongs to the LIF/OSM family.
    • Cellular localization
      Secreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • MGC20461 antibody
      • ONCM_HUMAN antibody
      • Oncostatin M antibody
      • Oncostatin-M antibody
      • OSM antibody
      see all

    References

    This product has been referenced in:
    See 1 Publication for this product

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Answer

    For the detection antibody you can use a biotinylated secondary such as ab6720 (Goat polyclonal to Rabbit IgG H&L (biotin))-one of the compatible secondaries for ab9633 listed at the bottom of the datasheet.

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    Answer

    Thank you for your enquiry. Below is a general Direct ELISA protocol which should be helpful for you. If you have any more questions, please contact us again. Direct ELISA: To detect hOncostatin-M by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents (located at the bottom of the datasheet), allows the detection of 0.2 - 0.4 ng/well of recombinant hOncostatin-M. 1. Dilute Ag into coating buffer (1 x PBS, pH 7.4). Add 100 ul/well. Incubate overnight at 4C. 2. Wash plate with wash buffer (1 x PBS, 0.05% Tween-20). Add 300 ul/well of blocking buffer ( 1 x PBS, 0.05% Tween-20, 1% BSA). Incubate for 2hrs at room emp. 3. Wash plate with wash buffer. Dilute detecting Ab (biotinylated antibody) to 1 ug/ml in diluting buffer (1 x PBS, 0.05% Tween-20, 1% BSA). Add 100 ul/well of detecting antibody. Incubate for 2 hrs at room temp. 4. Wash plate with wash buffer. Dilute Avidin Peroxidase 1:2000 in dilutent. Add 100 ul/well. 5. Wash plate with wash buffer. Add 100 ul/well of ABTS substrate solution (10.5 Citric Acid, 150 mg ABTS in 500 ml, pH to 4.35). Add 100 ul/well of ABTS substrate solution. 6. Read absorbance at 405 nm.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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