Product nameAnti-ONECUT2 antibody
DescriptionRabbit polyclonal to ONECUT2
Specificityab28466 recognises one cut domain family member 2 (ONECUT2)
Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
Species reactivityReacts with: Mouse, Human, Zebrafish
Predicted to work with: Rat, Dog
- Jurkat cell lysate. Human kidney
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab28466 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1.25 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|IHC-P||Use at an assay dependent concentration.|
RelevanceONECUT2 is a member of the transcription factors of the ONECUT class, whose prototype is hepatocyte nuclear factor (HNF)-6. The distribution of OC-2 mRNA in humans is tissue-restricted, the strongest expression being detected in the liver and skin. ONECUT2 participates in the network of transcription factors required for liver differentiation and metabolism.
- HGNC:8139 antibody
- MGC120377 antibody
- MGC120378 antibody
Anti-ONECUT2 antibody (ab28466) at 1.25 µg/ml + Jurkat cell lysate
Predicted band size: 52 kDa
Observed band size: 52 kDa
ONECUT2 expression detected in Jurkat cell lysate. Gel concentration is 12%. An HRP-conjugated anti-rabbit has been used as secondary antibody (suggested dilution: 1:50000-100000)
ONECUT2 expression detected in human kidney sections with 4-8ug/ml ab28466. The arrows indicate positive staining (epithelial cells of renal tubule). Magnification: 40X.
All lanes : Anti-ONECUT2 antibody (ab28466) at 1.25 µg/ml
Lane 1 : Marker
Lane 2 : Zebrafish liver homogenate (20ug)
Lane 3 : Mouse liver homogenate (20ug)
Lane 4 : JURKAT (Human T cell lymphoblast-like cell line) Whole Cell Lysate (20ug)
All lanes : Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 52 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
This product has been referenced in:
- Lu T et al. Blockade of ONECUT2 expression in ovarian cancer inhibited tumor cell proliferation, migration, invasion and angiogenesis. Cancer Sci 109:2221-2234 (2018). Read more (PubMed: 29737581) »
- Ao R et al. Altered microRNA-9 Expression Level is Directly Correlated with Pathogenesis of Nonalcoholic Fatty Liver Disease by Targeting Onecut2 and SIRT1. Med Sci Monit 22:3804-3819 (2016). WB ; Human . Read more (PubMed: 27756894) »