• Product name

    Anti-OPA1 antibody [1E81D9]
    See all OPA1 primary antibodies
  • Description

    Mouse monoclonal [1E81D9] to OPA1
  • Host species

  • Tested applications

    Suitable for: WB, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is considered to be commercially sensitive.

  • General notes

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.



Our Abpromise guarantee covers the use of ab119685 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 112 kDa.
Flow Cyt Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.



  • Function

    Dynamin-related GTPase required for mitochondrial fusion and regulation of apoptosis. May form a diffusion barrier for proteins stored in mitochondrial cristae. Proteolytic processing in response to intrinsic apoptotic signals may lead to disassembly of OPA1 oligomers and release of the caspase activator cytochrome C (CYCS) into the mitochondrial intermembrane space.
  • Tissue specificity

    Highly expressed in retina. Also expressed in brain, testis, heart and skeletal muscle. Isoform 1 expressed in retina, skeletal muscle, heart, lung, ovary, colon, thyroid gland, leukocytes and fetal brain. Isoform 2 expressed in colon, liver, kidney, thyroid gland and leukocytes. Low levels of all isoforms expressed in a variety of tissues.
  • Involvement in disease

    Defects in OPA1 are a cause of optic atrophy type 1 (OPA1) [MIM:165500]. OPA1 is a dominantly inherited optic neuropathy occurring in 1 in 50,000 individuals that features progressive loss in visual acuity leading, in many cases, to legal blindness.
    Defects in OPA1 are the cause of optic atrophy 1 with deafness (OPA1D) [MIM:125250]. Some individuals with mutations in OPA1 manifest also ophthalmoplegia and myopathy.
  • Sequence similarities

    Belongs to the dynamin family.
  • Post-translational

    PARL-dependent proteolytic processing releases an antiapoptotic soluble form not required for mitochondrial fusion.
  • Cellular localization

    Mitochondrion inner membrane. Mitochondrion intermembrane space.
  • Information by UniProt
  • Database links

  • Alternative names

    • Dynamin like 120 kDa protein antibody
    • Dynamin like 120 kDa protein, mitochondrial antibody
    • Dynamin-like 120 kDa protein, form S1 antibody
    • FLJ12460 antibody
    • Juvenile kjer type optic atrophy antibody
    • KIAA0567 antibody
    • KJER type antibody
    • Large GTP binding protein antibody
    • largeG antibody
    • MGM1 antibody
    • Mitochondrial dynamin like 120 kDa protein antibody
    • Mitochondrial dynamin like GTPase antibody
    • NPG antibody
    • NTG antibody
    • OAK antibody
    • OPA 1 antibody
    • opa1 antibody
    • OPA1 gene antibody
    • OPA1_HUMAN antibody
    • Optic atrophy 1 (autosomal dominant) antibody
    • OPTIC ATROPHY 1 antibody
    • Optic atrophy 1 gene protein antibody
    • Optic atrophy 1 homolog (human) antibody
    • Optic atrophy protein 1 antibody
    • Optic atrophy protein 1 homolog antibody
    see all


  • Overlay histogram showing SH-SY5Y cells stained with ab119685 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab119685, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • All lanes : Anti-OPA1 antibody [1E81D9] (ab119685) at 1 µg/ml

    Lane 1 : whole cell lysates from HeLa cells(human)
    Lane 2 : whole cell lysates from H4IIE cells(rat)
    Lane 3 : whole cell lysates from MEF cells(mouse)

    Lysates/proteins at 30 µg per lane.

    All lanes : HRP goat anti-mouse at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 112 kDa


This product has been referenced in:

  • Yen JH  et al. Activation of dynamin-related protein 1 - dependent mitochondria fragmentation and suppression of osteosarcoma by cryptotanshinone. J Exp Clin Cancer Res 38:42 (2019). Read more (PubMed: 30691497) »
  • Bartsakoulia M  et al. A novel mechanism causing imbalance of mitochondrial fusion and fission in human myopathies. Hum Mol Genet 27:1186-1195 (2018). Read more (PubMed: 29361167) »
See all 5 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Western blot
Human Tissue lysate - whole (placenta)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
100 µg
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Dr. Polina Vishnyakova

Verified customer

Submitted Jun 21 2016


The epitope for this antibody hasn’t been mapped but the immunogen was the C-terminal region common to all of the OPA1 splice variants. Therefore, the antibody is expected to bind to all splice variants.

There are 8 different splice isoforms of OPA1, see Figure attached. The two bands present in the WB image on the datasheet indicate that the antibody is detecting simultaneously 2 isoforms present in these cell lines, however, we are unable to determine which isoforms they are.

Read More

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