Key features and details
- Mouse monoclonal [1E81D9] to OPA1
- Suitable for: WB, Flow Cyt
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Product nameAnti-OPA1 antibody [1E81D9]
See all OPA1 primary antibodies
DescriptionMouse monoclonal [1E81D9] to OPA1
Tested applicationsSuitable for: WB, Flow Cytmore details
Unsuitable for: ELISA or IP
Species reactivityReacts with: Mouse, Rat, Human
Recombinant fragment. This information is considered to be commercially sensitive.
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Storage bufferPreservative: 0.02% Sodium azide
Constituent: 99% HEPES buffered saline
Concentration information loading...
PurityAmmonium Sulphate Precipitation
Purification notesPurity is near homogeneity as judged by SDS-PAGE. ab119685 was produced in vitro using hybridomas grown in serum-free medium, and then concentrated by ammonium sulfate precipitation.
Light chain typekappa
Our Abpromise guarantee covers the use of ab119685 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 112 kDa.|
|Flow Cyt||Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
FunctionDynamin-related GTPase required for mitochondrial fusion and regulation of apoptosis. May form a diffusion barrier for proteins stored in mitochondrial cristae. Proteolytic processing in response to intrinsic apoptotic signals may lead to disassembly of OPA1 oligomers and release of the caspase activator cytochrome C (CYCS) into the mitochondrial intermembrane space.
Tissue specificityHighly expressed in retina. Also expressed in brain, testis, heart and skeletal muscle. Isoform 1 expressed in retina, skeletal muscle, heart, lung, ovary, colon, thyroid gland, leukocytes and fetal brain. Isoform 2 expressed in colon, liver, kidney, thyroid gland and leukocytes. Low levels of all isoforms expressed in a variety of tissues.
Involvement in diseaseDefects in OPA1 are a cause of optic atrophy type 1 (OPA1) [MIM:165500]. OPA1 is a dominantly inherited optic neuropathy occurring in 1 in 50,000 individuals that features progressive loss in visual acuity leading, in many cases, to legal blindness.
Defects in OPA1 are the cause of optic atrophy 1 with deafness (OPA1D) [MIM:125250]. Some individuals with mutations in OPA1 manifest also ophthalmoplegia and myopathy.
Sequence similaritiesBelongs to the dynamin family.
modificationsPARL-dependent proteolytic processing releases an antiapoptotic soluble form not required for mitochondrial fusion.
Cellular localizationMitochondrion inner membrane. Mitochondrion intermembrane space.
- Information by UniProt
- Dynamin like 120 kDa protein antibody
- Dynamin like 120 kDa protein, mitochondrial antibody
- Dynamin-like 120 kDa protein, form S1 antibody
Overlay histogram showing SH-SY5Y cells stained with ab119685 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab119685, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
All lanes : Anti-OPA1 antibody [1E81D9] (ab119685) at 1 µg/ml
Lane 1 : whole cell lysates from HeLa cells(human)
Lane 2 : whole cell lysates from H4IIE cells(rat)
Lane 3 : whole cell lysates from MEF cells(mouse)
Lysates/proteins at 30 µg per lane.
All lanes : HRP goat anti-mouse at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 112 kDa
ab119685 has been referenced in 7 publications.
- Kochi C et al. Simulated vehicle exhaust exposure (SVEE) in rats impairs renal mitochondrial function. Clin Exp Hypertens 42:571-579 (2020). PubMed: 32423257
- Santos-Alves E et al. Physical exercise positively modulates DOX-induced hepatic oxidative stress, mitochondrial dysfunction and quality control signaling. Mitochondrion 47:103-113 (2019). PubMed: 31170523
- Yen JH et al. Activation of dynamin-related protein 1 - dependent mitochondria fragmentation and suppression of osteosarcoma by cryptotanshinone. J Exp Clin Cancer Res 38:42 (2019). PubMed: 30691497
- Bartsakoulia M et al. A novel mechanism causing imbalance of mitochondrial fusion and fission in human myopathies. Hum Mol Genet 27:1186-1195 (2018). PubMed: 29361167
- Takagi S et al. Ipragliflozin improves mitochondrial abnormalities in renal tubules induced by a high-fat diet. J Diabetes Investig N/A:N/A (2018). WB ; Mouse . PubMed: 29352520
- Vishnyakova PA et al. Alterations in antioxidant system, mitochondrial biogenesis and autophagy in preeclamptic myometrium. BBA Clin 8:35-42 (2017). PubMed: 28736722
- Pfeffer G et al. Mutations in the SPG7 gene cause chronic progressive external ophthalmoplegia through disordered mitochondrial DNA maintenance. Brain 137:1323-36 (2014). PubMed: 24727571