Recombinant Anti-OPA1 antibody [EPR11057(B)] (ab157457)
![Immunohistochemistry (Frozen sections) - Anti-OPA1 antibody [EPR11057(B)] (ab157457)](https://www.abcam.com/ps/products/157/ab157457/Images/ab157457-8-anti-opa1-antibody-epr11057-20-immunohistochemistry-frozen-retina-mouse.jpg "Immunohistochemistry (Frozen sections) - Anti-OPA1 antibody [EPR11057(B)] (ab157457)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11057(B)] to OPA1
- Suitable for: WB, IHC-P, IHC-Fr
- Reacts with: Mouse, Human
Overview
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Product name
Anti-OPA1 antibody [EPR11057(B)]
See all OPA1 primary antibodies -
Description
Rabbit monoclonal [EPR11057(B)] to OPA1 -
Host species
Rabbit -
Specificity
Recent lab testing showed that the antibody detects the band of interest at the proper molecular weight in several cell and tissue lysates. However, the antibody also detects a band at about 50kDa. We could not find in the literature any references on what this band could be. On our test, the 50kDa band detections decreased by decreasing the antibody concentration. -
Tested applications
Suitable for: WB, IHC-P, IHC-Frmore details
Unsuitable for: ICC/IF or IP -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Sheep, Rabbit, Horse, Chicken, Guinea pig, Pig, Non human primates, Chinese hamster, Common marmoset, Bat
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Immunogen
Synthetic peptide within Human OPA1 aa 900 to the C-terminus. The exact sequence is proprietary.
Database link: O60313 -
Positive control
- WB: HeLa, HepG2, A431 cell lysates; IHC-P: Human brain and retina tissue; IHC-Fr: Mouse retina tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR11057(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab157457 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (4) |
1/1000 - 1/5000. Predicted molecular weight: 112 kDa.
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| IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
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| IHC-Fr |
1/250.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20) |
| Notes |
|---|
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WB
1/1000 - 1/5000. Predicted molecular weight: 112 kDa. |
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IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
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IHC-Fr
1/250. Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20) |
Application notes
Is unsuitable for ICC/IF or IP.
Target
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Function
Dynamin-related GTPase required for mitochondrial fusion and regulation of apoptosis. May form a diffusion barrier for proteins stored in mitochondrial cristae. Proteolytic processing in response to intrinsic apoptotic signals may lead to disassembly of OPA1 oligomers and release of the caspase activator cytochrome C (CYCS) into the mitochondrial intermembrane space. -
Tissue specificity
Highly expressed in retina. Also expressed in brain, testis, heart and skeletal muscle. Isoform 1 expressed in retina, skeletal muscle, heart, lung, ovary, colon, thyroid gland, leukocytes and fetal brain. Isoform 2 expressed in colon, liver, kidney, thyroid gland and leukocytes. Low levels of all isoforms expressed in a variety of tissues. -
Involvement in disease
Defects in OPA1 are a cause of optic atrophy type 1 (OPA1) [MIM:165500]. OPA1 is a dominantly inherited optic neuropathy occurring in 1 in 50,000 individuals that features progressive loss in visual acuity leading, in many cases, to legal blindness.
Defects in OPA1 are the cause of optic atrophy 1 with deafness (OPA1D) [MIM:125250]. Some individuals with mutations in OPA1 manifest also ophthalmoplegia and myopathy. -
Sequence similarities
Belongs to the dynamin family. -
Post-translational
modificationsPARL-dependent proteolytic processing releases an antiapoptotic soluble form not required for mitochondrial fusion. -
Cellular localization
Mitochondrion inner membrane. Mitochondrion intermembrane space. - Information by UniProt
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Database links
- Entrez Gene: 424900 Chicken
- Entrez Gene: 4976 Human
- Entrez Gene: 74143 Mouse
- Entrez Gene: 171116 Rat
- Omim: 605290 Human
- SwissProt: O60313 Human
- SwissProt: P58281 Mouse
- SwissProt: Q2TA68 Rat
see all -
Alternative names
- Dynamin like 120 kDa protein antibody
- Dynamin like 120 kDa protein, mitochondrial antibody
- Dynamin-like 120 kDa protein, form S1 antibody
see all
Images
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Immunohistochemistry (Frozen sections) - Anti-OPA1 antibody [EPR11057(B)] (ab157457)Immunohistochemistry (Frozen sections) analysis of mouse retina tissue sections labeling OPA1 with Purified ab157457 at 1/250 (1.1 µg/ml).Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. DAPI was used as a counterstain.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OPA1 antibody [EPR11057(B)] (ab157457)](https://www.abcam.com/ps/products/157/ab157457/Images/ab157457-164489-anti-opa1-antibody-epr11057b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OPA1 antibody [EPR11057(B)] (ab157457)Immunohistochemical analysis of Paraffin-embedded Human brain tissue labeling OPA1 with ab157457 at 1/250 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Western blot - Anti-OPA1 antibody [EPR11057(B)] (ab157457)](https://www.abcam.com/ps/products/157/ab157457/Images/ab157457-349747-antiopa1-antibody-EPR11057B-western-blot-epidermoid-carcinoma-human.JPG)
Western blot - Anti-OPA1 antibody [EPR11057(B)] (ab157457)Anti-OPA1 antibody [EPR11057(B)] (ab157457) at 1/1000 dilution + A431 (Human epidermoid carcinoma epithelial cell) whole cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 112 kDa
Observed band size: 85-100 kDa why is the actual band size different from the predicted?
Exposure time: 180 secondsBlocking/Diluting buffer and concentration: 5% NFDM/TBST
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OPA1 antibody [EPR11057(B)] (ab157457)](https://www.abcam.com/ps/products/157/ab157457/Images/ab157457-164488-anti-opa1-antibody-epr11057b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OPA1 antibody [EPR11057(B)] (ab157457)Immunohistochemical analysis of Paraffin-embedded Human retina tissue labeling OPA1 with ab157457 at 1/250 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Western blot - Anti-OPA1 antibody [EPR11057(B)] (ab157457)](https://www.abcam.com/ps/products/157/ab157457/Images/ab157457-164490-anti-opa1-antibody-epr11057b-western-blot.jpg)
Western blot - Anti-OPA1 antibody [EPR11057(B)] (ab157457)All lanes : Anti-OPA1 antibody [EPR11057(B)] (ab157457) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : A431 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 112 kDa -
![Anti-OPA1 antibody [EPR11057(B)] (ab157457)](https://www.abcam.com/ps/products/157/ab157457/Images/ab157457-9-benefits-of-recombinant-antibodies.png)
Anti-OPA1 antibody [EPR11057(B)] (ab157457)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (20)
ab157457 has been referenced in 20 publications.
- Ma W et al. Silver Nanoparticle Exposure Causes Pulmonary Structural Damage and Mitochondrial Dynamic Imbalance in the Rat: Protective Effects of Sodium Selenite. Int J Nanomedicine 15:633-645 (2020). PubMed: 32099356
- Yang J et al. T-2 Toxin-Induced Oxidative Stress Leads to Imbalance of Mitochondrial Fission and Fusion to Activate Cellular Apoptosis in the Human Liver 7702 Cell Line. Toxins (Basel) 12:N/A (2020). PubMed: 31936883
- Meza-Torres C et al. Resveratrol Regulates the Expression of Genes Involved in CoQ Synthesis in Liver in Mice Fed with High Fat Diet. Antioxidants (Basel) 9:N/A (2020). PubMed: 32429295
- Chen T et al. ROS-Mediated Mitochondrial Dysfunction and ER Stress Contribute to Compression-Induced Neuronal Injury. Neuroscience 416:268-280 (2019). PubMed: 31425734
- Liu JX et al. Disturbance of mitochondrial dynamics and mitophagy in sepsis-induced acute kidney injury. Life Sci 235:116828 (2019). PubMed: 31479679