Recombinant
RabMAb

Recombinant Anti-OPA1 antibody [EPR11057(B)] (ab157457)

Rabbit recombinant monoclonal OPA1 antibody [EPR11057(B)]. Validated in WB, IHC, Flow Cyt, ICC/IF and tested in Mouse, Rat, Human. Cited in 7 publication(s). Independently reviewed in 3 review(s).

Reviews (3) Submit a question References (11)

Overview

  • Product name

    Anti-OPA1 antibody [EPR11057(B)]
    See all OPA1 primary antibodies

  • Description

    Rabbit monoclonal [EPR11057(B)] to OPA1

  • Host species

    Rabbit

  • Specificity

    Recent lab testing showed that the antibody detects the band of interest at the proper molecular weight in several cell and tissue lysates. However, the antibody also detects a band at about 50kDa. We could not find in the literature any references on what this band could be. On our test, the 50kDa band detections decreased by decreasing the antibody concentration.

  • Tested applications

    Suitable for: WB, IHC-P, IHC-Frmore details
    Unsuitable for: ICC/IF or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Sheep, Rabbit, Horse, Chicken, Guinea pig, Pig, Non human primates, Chinese hamster, Common marmoset, Bat

  • Immunogen

    Synthetic peptide within Human OPA1 aa 900 to the C-terminus. The exact sequence is proprietary.
    Database link: O60313

  • Positive control

    • WB: HeLa, HepG2, A431 cell lysates; IHC-P: Human brain and retina tissue; IHC-Fr: Mouse retina tissue.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab157457 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 112 kDa.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr 1/250.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)
  • Application notes
    Is unsuitable for ICC/IF or IP.

    • Target

    • Function

      Dynamin-related GTPase required for mitochondrial fusion and regulation of apoptosis. May form a diffusion barrier for proteins stored in mitochondrial cristae. Proteolytic processing in response to intrinsic apoptotic signals may lead to disassembly of OPA1 oligomers and release of the caspase activator cytochrome C (CYCS) into the mitochondrial intermembrane space.

    • Tissue specificity

      Highly expressed in retina. Also expressed in brain, testis, heart and skeletal muscle. Isoform 1 expressed in retina, skeletal muscle, heart, lung, ovary, colon, thyroid gland, leukocytes and fetal brain. Isoform 2 expressed in colon, liver, kidney, thyroid gland and leukocytes. Low levels of all isoforms expressed in a variety of tissues.

    • Involvement in disease

      Defects in OPA1 are a cause of optic atrophy type 1 (OPA1) [MIM:165500]. OPA1 is a dominantly inherited optic neuropathy occurring in 1 in 50,000 individuals that features progressive loss in visual acuity leading, in many cases, to legal blindness.
      Defects in OPA1 are the cause of optic atrophy 1 with deafness (OPA1D) [MIM:125250]. Some individuals with mutations in OPA1 manifest also ophthalmoplegia and myopathy.

    • Sequence similarities

      Belongs to the dynamin family.

    • Post-translational
      modifications

      PARL-dependent proteolytic processing releases an antiapoptotic soluble form not required for mitochondrial fusion.

    • Cellular localization

      Mitochondrion inner membrane. Mitochondrion intermembrane space.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • Dynamin like 120 kDa protein antibody
      • Dynamin like 120 kDa protein, mitochondrial antibody
      • Dynamin-like 120 kDa protein, form S1 antibody
      • FLJ12460 antibody
      • Juvenile kjer type optic atrophy antibody
      • KIAA0567 antibody
      • KJER type antibody
      • Large GTP binding protein antibody
      • largeG antibody
      • MGM1 antibody
      • Mitochondrial dynamin like 120 kDa protein antibody
      • Mitochondrial dynamin like GTPase antibody
      • NPG antibody
      • NTG antibody
      • OAK antibody
      • OPA 1 antibody
      • opa1 antibody
      • OPA1 gene antibody
      • OPA1_HUMAN antibody
      • Optic atrophy 1 (autosomal dominant) antibody
      • OPTIC ATROPHY 1 antibody
      • Optic atrophy 1 gene protein antibody
      • Optic atrophy 1 homolog (human) antibody
      • Optic atrophy protein 1 antibody
      • Optic atrophy protein 1 homolog antibody
      see all

    Images

    • Immunohistochemistry (Frozen sections) - Anti-OPA1 antibody [EPR11057(B)] (ab157457)
      Immunohistochemistry (Frozen sections) - Anti-OPA1 antibody [EPR11057(B)] (ab157457)
      Immunohistochemistry (Frozen sections) analysis of mouse retina tissue sections labeling OPA1 with Purified ab157457 at 1/250 (1.1 µg/ml).Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. DAPI was used as a counterstain.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OPA1 antibody [EPR11057(B)] (ab157457)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OPA1 antibody [EPR11057(B)] (ab157457)

      Immunohistochemical analysis of Paraffin-embedded Human brain tissue labeling OPA1 with ab157457 at 1/250 dilution.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Western blot - Anti-OPA1 antibody [EPR11057(B)] (ab157457)
      Western blot - Anti-OPA1 antibody [EPR11057(B)] (ab157457)
      Anti-OPA1 antibody [EPR11057(B)] (ab157457) at 1/1000 dilution + A431 (Human epidermoid carcinoma epithelial cell) whole cell lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

      Predicted band size: 112 kDa
      Observed band size: 85-100 kDa
      why is the actual band size different from the predicted?


      Exposure time: 180 seconds


      Blocking/Diluting buffer and concentration: 5% NFDM/TBST

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OPA1 antibody [EPR11057(B)] (ab157457)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OPA1 antibody [EPR11057(B)] (ab157457)

      Immunohistochemical analysis of Paraffin-embedded Human retina tissue labeling OPA1 with ab157457 at 1/250 dilution.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Western blot - Anti-OPA1 antibody [EPR11057(B)] (ab157457)
      Western blot - Anti-OPA1 antibody [EPR11057(B)] (ab157457)
      All lanes : Anti-OPA1 antibody [EPR11057(B)] (ab157457) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : A431 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 112 kDa

    References

    This product has been referenced in:

    • Yan QW  et al. Effects of treadmill exercise on mitochondrial fusion and fission in the hippocampus of APP/PS1 mice. Neurosci Lett 701:84-91 (2019). Read more (PubMed: 30796962) »
    • Hall A  et al. Perturbation of mitochondrial bioenergetics by polycations counteracts resistance to BRAFE600 inhibition in melanoma cells. J Control Release 309:158-172 (2019). Read more (PubMed: 31348978) »
    See all 11 Publications for this product

    Publishing research using ab157457? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    1-3 of 3 Abreviews or Q&A

    Immunocytochemistry abreview for Anti-OPA1 antibody [EPR11057(B)]

     Excellent
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Sample
    Rat Cultured Cells (Cardiomyocytes)
    Permeabilization
    Yes - Triton x-100, 0.03%
    Specification
    Cardiomyocytes
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 16 2018

    Western blot abreview for Anti-OPA1 antibody [EPR11057(B)]

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HEK293)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    40 µg
    Specification
    HEK293
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 08 2016

    Western blot abreview for Anti-OPA1 antibody [EPR11057(B)]

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Cell lysate - whole cell (Heart)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    40 µg
    Specification
    Heart
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 08 2016

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