Anti-OPA1 antibody [EPR11057(B)] (ab157457)
- Datasheet
- Specific References (5)
- Protocols
Overview
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Product nameAnti-OPA1 antibody [EPR11057(B)]
See all OPA1 primary antibodies -
DescriptionRabbit monoclonal [EPR11057(B)] to OPA1
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Host speciesRabbit
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SpecificityRecent lab testing showed that the antibody detects the band of interest at the proper molecular weight in several cell and tissue lysates. However, the antibody also detects a band at about 50kDa. We could not find in the literature any references on what this band could be. On our test, the 50kDa band detections decreased by decreasing the antibody concentration.
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Tested applicationsSuitable for: ICC/IF, WB, IHC-P, Flow Cytmore details
Unsuitable for: IP -
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Sheep, Rabbit, Horse, Chicken, Guinea pig, Pig, Non human primates, Chinese hamster, Common marmoset, Bat
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Immunogen
Synthetic peptide, corresponding to residues in Human OPA1 (Uniprot: O60313).
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Positive control
- HeLa, HepG2, A431 cell lysates; Human brain and retina tissues; HepG2 and HeLa cells. This antibody gave a positive result when used in the following methanol fixed cell line: HCT116.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at -20ºC.
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Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEPR11057(B)
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IsotypeIgG
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Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Positive Controls
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Related Products
Applications
Our Abpromise guarantee covers the use of ab157457 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/200 - 1/500. | |
| WB | 1/1000 - 1/5000. Predicted molecular weight: 112 kDa. | |
| IHC-P | 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| Flow Cyt | 1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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FunctionDynamin-related GTPase required for mitochondrial fusion and regulation of apoptosis. May form a diffusion barrier for proteins stored in mitochondrial cristae. Proteolytic processing in response to intrinsic apoptotic signals may lead to disassembly of OPA1 oligomers and release of the caspase activator cytochrome C (CYCS) into the mitochondrial intermembrane space.
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Tissue specificityHighly expressed in retina. Also expressed in brain, testis, heart and skeletal muscle. Isoform 1 expressed in retina, skeletal muscle, heart, lung, ovary, colon, thyroid gland, leukocytes and fetal brain. Isoform 2 expressed in colon, liver, kidney, thyroid gland and leukocytes. Low levels of all isoforms expressed in a variety of tissues.
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Involvement in diseaseDefects in OPA1 are a cause of optic atrophy type 1 (OPA1) [MIM:165500]. OPA1 is a dominantly inherited optic neuropathy occurring in 1 in 50,000 individuals that features progressive loss in visual acuity leading, in many cases, to legal blindness.
Defects in OPA1 are the cause of optic atrophy 1 with deafness (OPA1D) [MIM:125250]. Some individuals with mutations in OPA1 manifest also ophthalmoplegia and myopathy. -
Sequence similaritiesBelongs to the dynamin family.
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Post-translational
modificationsPARL-dependent proteolytic processing releases an antiapoptotic soluble form not required for mitochondrial fusion. -
Cellular localizationMitochondrion inner membrane. Mitochondrion intermembrane space.
- Information by UniProt
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Database links
- Entrez Gene: 424900 Chicken
- Entrez Gene: 4976 Human
- Entrez Gene: 74143 Mouse
- Entrez Gene: 171116 Rat
- Omim: 605290 Human
- SwissProt: O60313 Human
- SwissProt: P58281 Mouse
- SwissProt: Q2TA68 Rat
see all -
Alternative names
- Dynamin like 120 kDa protein antibody
- Dynamin like 120 kDa protein, mitochondrial antibody
- Dynamin-like 120 kDa protein, form S1 antibody
see all
Images
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![Immunocytochemistry/ Immunofluorescence - Anti-OPA1 antibody [EPR11057(B)] (ab157457)](https://www.abcam.com/ps/products/157/ab157457/Images/ab157457-265975-ab157457-HT29methanol500.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-OPA1 antibody [EPR11057(B)] (ab157457)Immunocytochemistry/Immunofluorescence analysis of HT-29 (human colorectal adenocarcinoma) labelling OPA1 with purified ab157457 at 1/500. Cells were fixed with 100% methanol. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Ab150077). Nuclei counterstained with DAPI (blue).
Control: PBS only
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![Immunocytochemistry/ Immunofluorescence - Anti-OPA1 antibody [EPR11057(B)] (ab157457)](https://www.abcam.com/ps/products/157/ab157457/Images/ab157457-210478-ab157457GR1189996EDIT.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-OPA1 antibody [EPR11057(B)] (ab157457)ICC/IF image of ab157457 stained HCT116 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab157457 at overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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![Western blot - Anti-OPA1 antibody [EPR11057(B)] (ab157457)](https://www.abcam.com/ps/products/157/ab157457/Images/OPA1-Primary-antibodies-ab157457-2.jpg)
Western blot - Anti-OPA1 antibody [EPR11057(B)] (ab157457)All lanes : Anti-OPA1 antibody [EPR11057(B)] (ab157457) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : A431 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 112 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OPA1 antibody [EPR11057(B)] (ab157457)](https://www.abcam.com/ps/products/157/ab157457/Images/OPA1-Primary-antibodies-ab157457-3.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OPA1 antibody [EPR11057(B)] (ab157457)Immunohistochemical analysis of Paraffin-embedded Human brain tissue labeling OPA1 with ab157457 at 1/250 dilution. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OPA1 antibody [EPR11057(B)] (ab157457)](https://www.abcam.com/ps/products/157/ab157457/Images/OPA1-Primary-antibodies-ab157457-4.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OPA1 antibody [EPR11057(B)] (ab157457)Immunohistochemical analysis of Paraffin-embedded Human retina tissue labeling OPA1 with ab157457 at 1/250 dilution. -
![Flow Cytometry - Anti-OPA1 antibody [EPR11057(B)] (ab157457)](https://www.abcam.com/ps/products/157/ab157457/Images/OPA1-Primary-antibodies-ab157457-5.jpg)
Flow Cytometry - Anti-OPA1 antibody [EPR11057(B)] (ab157457)Flow cytometric analysis of permeabilized HeLa cells labeling OPA1 with ab157457 at 1/10 dilution (red) or a rabbit IgG negative (green).
Protocols
References
This product has been referenced in:
- Pansarasa O et al. Lymphoblastoid cell lines as a model to understand amyotrophic lateral sclerosis disease mechanisms. Dis Model Mech 11:N/A (2018). Read more (PubMed: 29419416) »
- Xi Y et al. MitoQ protects dopaminergic neurons in a 6-OHDA induced PD model by enhancing Mfn2-dependent mitochondrial fusion via activation of PGC-1a. Biochim Biophys Acta N/A:N/A (2018). WB . Read more (PubMed: 29842922) »
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"