Recombinant
RabMAb

Anti-OPA1 antibody [EPR11057(B)] (ab157457)

Overview

  • Product name
    Anti-OPA1 antibody [EPR11057(B)]
    See all OPA1 primary antibodies
  • Description
    Rabbit monoclonal [EPR11057(B)] to OPA1
  • Host species
    Rabbit
  • Specificity
    Recent lab testing showed that the antibody detects the band of interest at the proper molecular weight in several cell and tissue lysates. However, the antibody also detects a band at about 50kDa. We could not find in the literature any references on what this band could be. On our test, the 50kDa band detections decreased by decreasing the antibody concentration.
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-P, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Sheep, Rabbit, Horse, Chicken, Guinea pig, Pig, Non human primates, Chinese hamster, Common marmoset, Bat
  • Immunogen

    Synthetic peptide, corresponding to residues in Human OPA1 (Uniprot: O60313).

  • Positive control
    • HeLa, HepG2, A431 cell lysates; Human brain and retina tissues; HepG2 and HeLa cells. This antibody gave a positive result when used in the following methanol fixed cell line: HCT116.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab157457 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/200 - 1/500.
WB 1/1000 - 1/5000. Predicted molecular weight: 112 kDa.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      Dynamin-related GTPase required for mitochondrial fusion and regulation of apoptosis. May form a diffusion barrier for proteins stored in mitochondrial cristae. Proteolytic processing in response to intrinsic apoptotic signals may lead to disassembly of OPA1 oligomers and release of the caspase activator cytochrome C (CYCS) into the mitochondrial intermembrane space.
    • Tissue specificity
      Highly expressed in retina. Also expressed in brain, testis, heart and skeletal muscle. Isoform 1 expressed in retina, skeletal muscle, heart, lung, ovary, colon, thyroid gland, leukocytes and fetal brain. Isoform 2 expressed in colon, liver, kidney, thyroid gland and leukocytes. Low levels of all isoforms expressed in a variety of tissues.
    • Involvement in disease
      Defects in OPA1 are a cause of optic atrophy type 1 (OPA1) [MIM:165500]. OPA1 is a dominantly inherited optic neuropathy occurring in 1 in 50,000 individuals that features progressive loss in visual acuity leading, in many cases, to legal blindness.
      Defects in OPA1 are the cause of optic atrophy 1 with deafness (OPA1D) [MIM:125250]. Some individuals with mutations in OPA1 manifest also ophthalmoplegia and myopathy.
    • Sequence similarities
      Belongs to the dynamin family.
    • Post-translational
      modifications
      PARL-dependent proteolytic processing releases an antiapoptotic soluble form not required for mitochondrial fusion.
    • Cellular localization
      Mitochondrion inner membrane. Mitochondrion intermembrane space.
    • Information by UniProt
    • Database links
    • Alternative names
      • Dynamin like 120 kDa protein antibody
      • Dynamin like 120 kDa protein, mitochondrial antibody
      • Dynamin-like 120 kDa protein, form S1 antibody
      • FLJ12460 antibody
      • Juvenile kjer type optic atrophy antibody
      • KIAA0567 antibody
      • KJER type antibody
      • Large GTP binding protein antibody
      • largeG antibody
      • MGM1 antibody
      • Mitochondrial dynamin like 120 kDa protein antibody
      • Mitochondrial dynamin like GTPase antibody
      • NPG antibody
      • NTG antibody
      • OAK antibody
      • OPA 1 antibody
      • opa1 antibody
      • OPA1 gene antibody
      • OPA1_HUMAN antibody
      • Optic atrophy 1 (autosomal dominant) antibody
      • OPTIC ATROPHY 1 antibody
      • Optic atrophy 1 gene protein antibody
      • Optic atrophy 1 homolog (human) antibody
      • Optic atrophy protein 1 antibody
      • Optic atrophy protein 1 homolog antibody
      see all

    Images

    • Immunocytochemistry/Immunofluorescence analysis of HT-29 (human colorectal adenocarcinoma) labelling OPA1 with purified ab157457 at 1/500. Cells were fixed with 100% methanol. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Ab150077). Nuclei counterstained with DAPI (blue).

      Control: PBS only

    • ICC/IF image of ab157457 stained HCT116 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab157457 at overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • All lanes : Anti-OPA1 antibody [EPR11057(B)] (ab157457) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : A431 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 112 kDa

    • Immunohistochemical analysis of Paraffin-embedded Human brain tissue labeling OPA1 with ab157457 at 1/250 dilution.
    • Immunohistochemical analysis of Paraffin-embedded Human retina tissue labeling OPA1 with ab157457 at 1/250 dilution.
    • Flow cytometric analysis of permeabilized HeLa cells labeling OPA1 with ab157457 at 1/10 dilution (red) or a rabbit IgG negative (green).

    References

    This product has been referenced in:
    • Pansarasa O  et al. Lymphoblastoid cell lines as a model to understand amyotrophic lateral sclerosis disease mechanisms. Dis Model Mech 11:N/A (2018). Read more (PubMed: 29419416) »
    • Xi Y  et al. MitoQ protects dopaminergic neurons in a 6-OHDA induced PD model by enhancing Mfn2-dependent mitochondrial fusion via activation of PGC-1a. Biochim Biophys Acta N/A:N/A (2018). WB . Read more (PubMed: 29842922) »

    See all 5 Publications for this product

    Customer reviews and Q&As

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry
    Sample
    Rat Cultured Cells (Cardiomyocytes)
    Permeabilization
    Yes - Triton x-100, 0.03%
    Specification
    Cardiomyocytes
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Feb 16 2018

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HEK293)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    40 µg
    Specification
    HEK293
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
    Username

    Abcam user community

    Verified customer

    Submitted Aug 08 2016

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Rat Cell lysate - whole cell (Heart)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    40 µg
    Specification
    Heart
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
    Username

    Abcam user community

    Verified customer

    Submitted Aug 08 2016

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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