Recombinant Anti-OPA1 antibody [EPR11057(B)] - BSA and Azide free (ab240143)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11057(B)] to OPA1 - BSA and Azide free
- Suitable for: WB, IHC-P, IHC-Fr
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-OPA1 antibody [EPR11057(B)] - BSA and Azide free
See all OPA1 primary antibodies -
Description
Rabbit monoclonal [EPR11057(B)] to OPA1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, IHC-Frmore details
Unsuitable for: ICC/IF or IP -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Sheep, Rabbit, Horse, Chicken, Guinea pig, Pig, Non human primates, Chinese hamster, Common marmoset, Bat
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Immunogen
Synthetic peptide within Human OPA1. The exact sequence is proprietary.
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Positive control
- WB: HeLa, HepG2, A431 cell lysates; IHC-P: Human brain and retina tissue; IHC-Fr: Mouse retina tissue.
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General notes
ab240143 is the carrier-free version of ab157457. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab240143 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR11057(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Immunohistochemistry kits
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab240143 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use at an assay dependent concentration. Predicted molecular weight: 112 kDa. | |
| IHC-P | Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| IHC-Fr | Use at an assay dependent concentration. Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20) |
Target
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Function
Dynamin-related GTPase required for mitochondrial fusion and regulation of apoptosis. May form a diffusion barrier for proteins stored in mitochondrial cristae. Proteolytic processing in response to intrinsic apoptotic signals may lead to disassembly of OPA1 oligomers and release of the caspase activator cytochrome C (CYCS) into the mitochondrial intermembrane space. -
Tissue specificity
Highly expressed in retina. Also expressed in brain, testis, heart and skeletal muscle. Isoform 1 expressed in retina, skeletal muscle, heart, lung, ovary, colon, thyroid gland, leukocytes and fetal brain. Isoform 2 expressed in colon, liver, kidney, thyroid gland and leukocytes. Low levels of all isoforms expressed in a variety of tissues. -
Involvement in disease
Defects in OPA1 are a cause of optic atrophy type 1 (OPA1) [MIM:165500]. OPA1 is a dominantly inherited optic neuropathy occurring in 1 in 50,000 individuals that features progressive loss in visual acuity leading, in many cases, to legal blindness.
Defects in OPA1 are the cause of optic atrophy 1 with deafness (OPA1D) [MIM:125250]. Some individuals with mutations in OPA1 manifest also ophthalmoplegia and myopathy. -
Sequence similarities
Belongs to the dynamin family. -
Post-translational
modificationsPARL-dependent proteolytic processing releases an antiapoptotic soluble form not required for mitochondrial fusion. -
Cellular localization
Mitochondrion inner membrane. Mitochondrion intermembrane space. - Information by UniProt
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Database links
- Entrez Gene: 424900 Chicken
- Entrez Gene: 4976 Human
- Entrez Gene: 74143 Mouse
- Entrez Gene: 171116 Rat
- Omim: 605290 Human
- SwissProt: O60313 Human
- SwissProt: P58281 Mouse
- SwissProt: Q2TA68 Rat
see all -
Alternative names
- Dynamin like 120 kDa protein antibody
- Dynamin like 120 kDa protein, mitochondrial antibody
- Dynamin-like 120 kDa protein, form S1 antibody
see all
Images
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![Immunohistochemistry (Frozen sections) - Anti-OPA1 antibody [EPR11057(B)] - BSA and Azide free (ab240143)](https://www.abcam.com/ps/products/240/ab240143/Images/ab240143-382065-anti-opa1-antibody-epr11057-20-bsa-and-azide-free-immunohistochemistry-frozen-retina-mouse.jpg)
Immunohistochemistry (Frozen sections) - Anti-OPA1 antibody [EPR11057(B)] - BSA and Azide free (ab240143)Immunohistochemistry (Frozen sections) analysis of mouse retina tissue sections labeling OPA1 with Purified ab157457 at 1/250 (1.1 µg/ml).Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. DAPI was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab157457).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OPA1 antibody [EPR11057(B)] - BSA and Azide free (ab240143)](https://www.abcam.com/ps/products/240/ab240143/Images/ab240143-323090-anti-opa1-antibody-epr11057b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OPA1 antibody [EPR11057(B)] - BSA and Azide free (ab240143)Immunohistochemical analysis of Paraffin-embedded Human brain tissue labeling OPA1 with ab157457 at 1/250 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab157457).
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OPA1 antibody [EPR11057(B)] - BSA and Azide free (ab240143)](https://www.abcam.com/ps/products/240/ab240143/Images/ab240143-323089-anti-opa1-antibody-epr11057b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OPA1 antibody [EPR11057(B)] - BSA and Azide free (ab240143)Immunohistochemical analysis of Paraffin-embedded Human retina tissue labeling OPA1 with ab157457 at 1/250 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab157457).
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References (0)
ab240143 has not yet been referenced specifically in any publications.
