RecombinantRabMAb

Recombinant Anti-OPA1 antibody [EPR11057(B)] - BSA and Azide free (ab240143)

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Immunohistochemistry (Frozen sections) - Anti-OPA1 antibody [EPR11057(B)] - BSA and Azide free (ab240143)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OPA1 antibody [EPR11057(B)] - BSA and Azide free (ab240143)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OPA1 antibody [EPR11057(B)] - BSA and Azide free (ab240143)
  • Anti-OPA1 antibody [EPR11057(B)] - BSA and Azide free (ab240143)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11057(B)] to OPA1 - BSA and Azide free
  • Suitable for: WB, IHC-P, IHC-Fr
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Unconjugated

Overview

  • Product name

    Anti-OPA1 antibody [EPR11057(B)] - BSA and Azide free
    See all OPA1 primary antibodies

  • Description

    Rabbit monoclonal [EPR11057(B)] to OPA1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, IHC-Frmore details
    Unsuitable for: ICC/IF or IP

  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Sheep, Rabbit, Horse, Chicken, Guinea pig, Pig, Non human primates, Chinese hamster, Common marmoset, Bat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, HepG2, A431 cell lysates; IHC-P: Human brain and retina tissue; IHC-Fr: Mouse retina tissue.

  • General notes

    ab240143 is the carrier-free version of ab157457.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab240143 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use at an assay dependent concentration. Predicted molecular weight: 112 kDa.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr
Use at an assay dependent concentration.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)
Notes
WB
Use at an assay dependent concentration. Predicted molecular weight: 112 kDa.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr
Use at an assay dependent concentration.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)
Application notes
Is unsuitable for ICC/IF or IP.

Target

  • Function

    Dynamin-related GTPase required for mitochondrial fusion and regulation of apoptosis. May form a diffusion barrier for proteins stored in mitochondrial cristae. Proteolytic processing in response to intrinsic apoptotic signals may lead to disassembly of OPA1 oligomers and release of the caspase activator cytochrome C (CYCS) into the mitochondrial intermembrane space.

  • Tissue specificity

    Highly expressed in retina. Also expressed in brain, testis, heart and skeletal muscle. Isoform 1 expressed in retina, skeletal muscle, heart, lung, ovary, colon, thyroid gland, leukocytes and fetal brain. Isoform 2 expressed in colon, liver, kidney, thyroid gland and leukocytes. Low levels of all isoforms expressed in a variety of tissues.

  • Involvement in disease

    Defects in OPA1 are a cause of optic atrophy type 1 (OPA1) [MIM:165500]. OPA1 is a dominantly inherited optic neuropathy occurring in 1 in 50,000 individuals that features progressive loss in visual acuity leading, in many cases, to legal blindness.
    Defects in OPA1 are the cause of optic atrophy 1 with deafness (OPA1D) [MIM:125250]. Some individuals with mutations in OPA1 manifest also ophthalmoplegia and myopathy.

  • Sequence similarities

    Belongs to the dynamin family.

  • Post-translational
    modifications

    PARL-dependent proteolytic processing releases an antiapoptotic soluble form not required for mitochondrial fusion.

  • Cellular localization

    Mitochondrion inner membrane. Mitochondrion intermembrane space.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Dynamin like 120 kDa protein antibody
    • Dynamin like 120 kDa protein, mitochondrial antibody
    • Dynamin-like 120 kDa protein, form S1 antibody
    • FLJ12460 antibody
    • Juvenile kjer type optic atrophy antibody
    • KIAA0567 antibody
    • KJER type antibody
    • Large GTP binding protein antibody
    • largeG antibody
    • MGM1 antibody
    • Mitochondrial dynamin like 120 kDa protein antibody
    • Mitochondrial dynamin like GTPase antibody
    • NPG antibody
    • NTG antibody
    • OAK antibody
    • OPA 1 antibody
    • opa1 antibody
    • OPA1 gene antibody
    • OPA1_HUMAN antibody
    • Optic atrophy 1 (autosomal dominant) antibody
    • OPTIC ATROPHY 1 antibody
    • Optic atrophy 1 gene protein antibody
    • Optic atrophy 1 homolog (human) antibody
    • Optic atrophy protein 1 antibody
    • Optic atrophy protein 1 homolog antibody
    see all

Images

  • Immunohistochemistry (Frozen sections) - Anti-OPA1 antibody [EPR11057(B)] - BSA and Azide free (ab240143)
    Immunohistochemistry (Frozen sections) - Anti-OPA1 antibody [EPR11057(B)] - BSA and Azide free (ab240143)

    Immunohistochemistry (Frozen sections) analysis of mouse retina tissue sections labeling OPA1 with Purified ab157457 at 1/250 (1.1 µg/ml).Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. DAPI was used as a counterstain.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab157457).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OPA1 antibody [EPR11057(B)] - BSA and Azide free (ab240143)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OPA1 antibody [EPR11057(B)] - BSA and Azide free (ab240143)

    Immunohistochemical analysis of Paraffin-embedded Human brain tissue labeling OPA1 with ab157457 at 1/250 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab157457).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OPA1 antibody [EPR11057(B)] - BSA and Azide free (ab240143)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OPA1 antibody [EPR11057(B)] - BSA and Azide free (ab240143)

    Immunohistochemical analysis of Paraffin-embedded Human retina tissue labeling OPA1 with ab157457 at 1/250 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab157457).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Anti-OPA1 antibody [EPR11057(B)] - BSA and Azide free (ab240143)
    Anti-OPA1 antibody [EPR11057(B)] - BSA and Azide free (ab240143)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab240143? Please let us know so that we can cite the reference in this datasheet.

ab240143 has not yet been referenced specifically in any publications.

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