Anti-OPALIN antibody (ab121425)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-OPALIN antibody -
Description
Rabbit polyclonal to OPALIN -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
amino acids 55-141 (RRRSSIEAME ESDRPCEISE IDDNPKISEN PRRSPTHEKN TMGAQEAHIY VKTVAGSEEP VHDRYRPTIE MERRRGLWWL VPRLSLE) of Human OPALIN.
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Positive control
- Human cerebellum tissue; Transfected HEK293T cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab121425 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | 1/250 - 1/500. |
Target
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Tissue specificity
Brain specific. -
Cellular localization
Cell membrane. In the CNS, enriched in the myelin paranodal and inner loop membranes, but not that of the PNS. - Information by UniProt
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Database links
- Entrez Gene: 93377 Human
- SwissProt: Q96PE5 Human
- Unigene: 12449 Human
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Alternative names
- HTMP10 antibody
- Oligodendrocytic myelin paranodal and inner loop protein antibody
- OPALI_HUMAN antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OPALIN antibody (ab121425)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hippocampus tissue labelling OPALIN with ab121425. Staining shows moderate cytoplasmic positivity in glial cells. -
Western blot - Anti-OPALIN antibody (ab121425)All lanes : Anti-OPALIN antibody (ab121425) at 1/250 dilution
Lane 1 : Vector only transfected HEK293T cell lysate
Lane 2 : Lysate from transfected HEK293T cells overexpressing OPALIN with a C-terminal myc-DDK tag (~3.1 kDa)
Developed using the ECL technique.
Datasheets and documents
References
This product has been referenced in:
- Jäkel S et al. Altered human oligodendrocyte heterogeneity in multiple sclerosis. Nature 566:543-547 (2019). Read more (PubMed: 30747918) »
