Anti-OPALIN antibody (ab121425)
Key features and details
- Rabbit polyclonal to OPALIN
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-OPALIN antibody -
Description
Rabbit polyclonal to OPALIN -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
amino acids 55-141 (RRRSSIEAME ESDRPCEISE IDDNPKISEN PRRSPTHEKN TMGAQEAHIY VKTVAGSEEP VHDRYRPTIE MERRRGLWWL VPRLSLE) of Human OPALIN.
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Positive control
- Human cerebellum tissue; Transfected HEK293T cell lysate.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab121425 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | 1/250 - 1/500. |
Target
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Tissue specificity
Brain specific. -
Cellular localization
Cell membrane. In the CNS, enriched in the myelin paranodal and inner loop membranes, but not that of the PNS. - Information by UniProt
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Database links
- Entrez Gene: 93377 Human
- SwissProt: Q96PE5 Human
- Unigene: 12449 Human
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Alternative names
- HTMP10 antibody
- Oligodendrocytic myelin paranodal and inner loop protein antibody
- OPALI_HUMAN antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OPALIN antibody (ab121425)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hippocampus tissue labelling OPALIN with ab121425. Staining shows moderate cytoplasmic positivity in glial cells.
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Western blot - Anti-OPALIN antibody (ab121425)All lanes : Anti-OPALIN antibody (ab121425) at 1/250 dilution
Lane 1 : Vector only transfected HEK293T cell lysate
Lane 2 : Lysate from transfected HEK293T cells overexpressing OPALIN with a C-terminal myc-DDK tag (~3.1 kDa)
Developed using the ECL technique.
Protocols
Datasheets and documents
References (1)
ab121425 has been referenced in 1 publication.
- Jäkel S et al. Altered human oligodendrocyte heterogeneity in multiple sclerosis. Nature 566:543-547 (2019). PubMed: 30747918
