Key features and details
- Rabbit polyclonal to OPALIN
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-OPALIN antibody
DescriptionRabbit polyclonal to OPALIN
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
- Human cerebellum tissue; Transfected HEK293T cell lysate.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab121425 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/250 - 1/500.|
Tissue specificityBrain specific.
Cellular localizationCell membrane. In the CNS, enriched in the myelin paranodal and inner loop membranes, but not that of the PNS.
- Information by UniProt
- HTMP10 antibody
- Oligodendrocytic myelin paranodal and inner loop protein antibody
- OPALI_HUMAN antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hippocampus tissue labelling OPALIN with ab121425. Staining shows moderate cytoplasmic positivity in glial cells.
All lanes : Anti-OPALIN antibody (ab121425) at 1/250 dilution
Lane 1 : Vector only transfected HEK293T cell lysate
Lane 2 : Lysate from transfected HEK293T cells overexpressing OPALIN with a C-terminal myc-DDK tag (~3.1 kDa)
Developed using the ECL technique.
ab121425 has been referenced in 1 publication.
- Jäkel S et al. Altered human oligodendrocyte heterogeneity in multiple sclerosis. Nature 566:543-547 (2019). PubMed: 30747918