Overview

  • Product name
    Anti-OPALIN antibody
  • Description
    Rabbit polyclonal to OPALIN
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    amino acids 55-141 (RRRSSIEAME ESDRPCEISE IDDNPKISEN PRRSPTHEKN TMGAQEAHIY VKTVAGSEEP VHDRYRPTIE MERRRGLWWL VPRLSLE) of Human OPALIN.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • Human cerebellum tissue; Transfected HEK293T cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab121425 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.

Target

  • Tissue specificity
    Brain specific.
  • Cellular localization
    Cell membrane. In the CNS, enriched in the myelin paranodal and inner loop membranes, but not that of the PNS.
  • Information by UniProt
  • Database links
    • Alternative names
      • HTMP10 antibody
      • Oligodendrocytic myelin paranodal and inner loop protein antibody
      • OPALI_HUMAN antibody
      • OPALIN antibody
      • TMEM10 antibody
      • TMP10 antibody
      • Transmembrane protein 10 antibody
      • transmembrane protein TMP10 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OPALIN antibody (ab121425)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OPALIN antibody (ab121425)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hippocampus tissue labelling OPALIN with ab121425. Staining shows moderate cytoplasmic positivity in glial cells.
    • Western blot - Anti-OPALIN antibody (ab121425)
      Western blot - Anti-OPALIN antibody (ab121425)
      All lanes : Anti-OPALIN antibody (ab121425) at 1/250 dilution

      Lane 1 : Vector only transfected HEK293T cell lysate
      Lane 2 : Lysate from transfected HEK293T cells overexpressing OPALIN with a C-terminal myc-DDK tag (~3.1 kDa)

      Developed using the ECL technique.

    References

    ab121425 has not yet been referenced specifically in any publications.

    Publishing research using ab121425? Please let us know so that we can cite the reference in this datasheet.

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