Anti-OPHN1 antibody (ab229655)
Key features and details
- Rabbit polyclonal to OPHN1
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-OPHN1 antibody -
Description
Rabbit polyclonal to OPHN1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Chimpanzee, Orangutan -
Immunogen
Recombinant fragment corresponding to Human OPHN1 aa 1-300.
Database link: O60890 -
Positive control
- WB: A549 whole cell lysate. IHC-P: Human colon cancer and gastric cancer tissues.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab229655 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/20 - 1/200.
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WB |
1/1000 - 1/5000. Detects a band of approximately 92 kDa (predicted molecular weight: 92 kDa).
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Notes |
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IHC-P
1/20 - 1/200. |
WB
1/1000 - 1/5000. Detects a band of approximately 92 kDa (predicted molecular weight: 92 kDa). |
Target
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Relevance
Stimulates GTP hydrolysis of members of the Rho family. Could activates GTPase targets that are known to affect cell migration and outgrowth of axons and dendrites. -
Database links
- Entrez Gene: 4983 Human
- Entrez Gene: 94190 Mouse
- Entrez Gene: 312108 Rat
- Omim: 300127 Human
- SwissProt: Q7YQL6 Chimpanzee
- SwissProt: O60890 Human
- SwissProt: Q99J31 Mouse
- SwissProt: Q7YQL5 Orangutan
see all -
Alternative names
- ARHGAP41 antibody
- Mental retardation, X linked 60 antibody
- MRX60 antibody
see all
Images
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Anti-OPHN1 antibody (ab229655) at 1/1000 dilution + A549 (human lung carcinoma cell line) whole cell lysate
Secondary
Goat polyclonal to rabbit at 1/10000 dilution
Predicted band size: 92 kDa
Observed band size: 92 kDa -
Paraffin-embedded human colon cancer tissue stained for OPHN1 using ab229655 at 1/100 dilution in immunohistochemical analysis.
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Paraffin-embedded human gastric cancer tissue stained for OPHN1 using ab229655 at 1/100 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab229655 has been referenced in 1 publication.
- Liu J et al. Androgen deprivation‑induced OPHN1 amplification promotes castration‑resistant prostate cancer. Oncol Rep 47:N/A (2022). PubMed: 34738630