Overview

  • Product name

  • Description

    Rabbit polyclonal to OPN3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to Human OPN3 (internal sequence).

  • Positive control

    • WB: Extracts from mouse brain cells. ICC/IF: MCF-7 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab75285 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 45 kDa.
ELISA 1/5000.
ICC/IF 1/500 - 1/1000.

Target

  • Function

    May play a role in encephalic photoreception.
  • Tissue specificity

    Strongly expressed in brain. Highly expressed in the preoptic area and paraventricular nucleus of the hypothalamus. Shows highly patterned expression in other regions of the brain, being enriched in selected regions of the cerebral cortex, cerebellar Purkinje cells, a subset of striatal neurons, selected thalamic nuclei, and a subset of interneurons in the ventral horn of the spinal cord.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family. Opsin subfamily.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • ECPN antibody
    • Encephalopsin antibody
    • ERO antibody
    • NMO 1 antibody
    • NMO1 antibody
    • OPN 3 antibody
    • Opn3 antibody
    • OPN3_HUMAN antibody
    • Opsin 3 antibody
    • Opsin-3 antibody
    • Opsin3 antibody
    • Panopsin antibody
    see all

Images

  • All lanes : Anti-OPN3 antibody (ab75285) at 1/500 dilution

    Lane 1 : Extracts from mouse brain cells
    Lane 2 : Extracts from mouse brain cells with immunising peptide at 10 µg

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 45 kDa
    Observed band size: 45 kDa

  • ab75285 staining OPN3 in MCF-7 cells by Immunofluorescence. Image on right when blocking peptide added.

References

This product has been referenced in:

  • Castellano-Pellicena I  et al. Does blue light restore human epidermal barrier function via activation of Opsin during cutaneous wound healing? Lasers Surg Med 51:370-382 (2019). Read more (PubMed: 30168605) »
  • El Massri N  et al. Photobiomodulation reduces gliosis in the basal ganglia of aged mice. Neurobiol Aging 66:131-137 (2018). IHC-Fr ; Mouse . Read more (PubMed: 29571001) »
See all 5 Publications for this product

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