Product nameAnti-OPN3 antibody
See all OPN3 primary antibodies
DescriptionRabbit polyclonal to OPN3
Tested applicationsSuitable for: WB, ELISA, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthetic peptide corresponding to Human OPN3 (internal sequence).
- WB: Extracts from mouse brain cells. ICC/IF: MCF-7 cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.87% Sodium chloride, 50% Glycerol, PBS
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThe antibody was affinity purified from rabbit antiserum by affinity chromatography
Our Abpromise guarantee covers the use of ab75285 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 45 kDa.|
|ICC/IF||1/500 - 1/1000.|
FunctionMay play a role in encephalic photoreception.
Tissue specificityStrongly expressed in brain. Highly expressed in the preoptic area and paraventricular nucleus of the hypothalamus. Shows highly patterned expression in other regions of the brain, being enriched in selected regions of the cerebral cortex, cerebellar Purkinje cells, a subset of striatal neurons, selected thalamic nuclei, and a subset of interneurons in the ventral horn of the spinal cord.
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family. Opsin subfamily.
- Information by UniProt
- ECPN antibody
- Encephalopsin antibody
- ERO antibody
All lanes : Anti-OPN3 antibody (ab75285) at 1/500 dilution
Lane 1 : Extracts from mouse brain cells
Lane 2 : Extracts from mouse brain cells with immunising peptide at 10 µg
Lysates/proteins at 10 µg per lane.
Predicted band size: 45 kDa
Observed band size: 45 kDa
ab75285 staining OPN3 in MCF-7 cells by Immunofluorescence. Image on right when blocking peptide added.
This product has been referenced in:
- Castellano-Pellicena I et al. Does blue light restore human epidermal barrier function via activation of Opsin during cutaneous wound healing? Lasers Surg Med 51:370-382 (2019). Read more (PubMed: 30168605) »
- El Massri N et al. Photobiomodulation reduces gliosis in the basal ganglia of aged mice. Neurobiol Aging 66:131-137 (2018). IHC-Fr ; Mouse . Read more (PubMed: 29571001) »