Anti-OPN3 antibody - N-terminal (ab228748)
Key features and details
- Rabbit polyclonal to OPN3 - N-terminal
- Suitable for: WB, IHC-P, IHC-Fr
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-OPN3 antibody - N-terminal
See all OPN3 primary antibodies -
Description
Rabbit polyclonal to OPN3 - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, IHC-Frmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide within Human OPN3 (N terminal). The exact sequence is proprietary. (Carrier-protein conjugated).
Database link: Q9H1Y3 -
Positive control
- WB: A431 whole cell lysate. IHC-P: Human breast cancer tissue. IHC-Fr: Adult mouse retina tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab228748 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 45 kDa.
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IHC-P |
1/100 - 1/1000.
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IHC-Fr |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 45 kDa. |
IHC-P
1/100 - 1/1000. |
IHC-Fr
1/100 - 1/1000. |
Target
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Function
May play a role in encephalic photoreception. -
Tissue specificity
Strongly expressed in brain. Highly expressed in the preoptic area and paraventricular nucleus of the hypothalamus. Shows highly patterned expression in other regions of the brain, being enriched in selected regions of the cerebral cortex, cerebellar Purkinje cells, a subset of striatal neurons, selected thalamic nuclei, and a subset of interneurons in the ventral horn of the spinal cord. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. Opsin subfamily. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 23596 Human
- Entrez Gene: 13603 Mouse
- Omim: 606695 Human
- SwissProt: Q9H1Y3 Human
- SwissProt: Q9WUK7 Mouse
- Unigene: 409081 Human
- Unigene: 32744 Mouse
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Alternative names
- ECPN antibody
- Encephalopsin antibody
- ERO antibody
see all
Images
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Anti-OPN3 antibody - N-terminal (ab228748) at 1/1000 dilution + A431 (human epidermoid carcinoma cell line) whole cell lysate at 30 µg
Predicted band size: 45 kDa10% SDS-PAGE gel.
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Paraffin-embedded human breast cancer tissue stained for OPN3 using ab228748 at 1/250 dilution in immunohistochemical analysis.
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Frozen sectioned adult mouse retina tissue stained for OPN3 using ab228748 at 1/250 dilution in immunohistochemical analysis (green).
The nuclear counterstain is DAPI (blue). Beta Tubulin 3 is detected with a beta Tubulin 3 antibody at 1/250 dilution (red).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab228748 has been referenced in 4 publications.
- Zhu J et al. Negative pressure wound therapy enhances bone regeneration compared with conventional therapy in a rabbit radius gap-healing model. Exp Ther Med 21:474 (2021). PubMed: 33767769
- Li S et al. Total Flavonoids of Rhizoma Drynariae Promotes Differentiation of Osteoblasts and Growth of Bone Graft in Induced Membrane Partly by Activating Wnt/ß-Catenin Signaling Pathway. Front Pharmacol 12:675470 (2021). PubMed: 34122101
- Xia M et al. LncRNA AFAP1-AS1/miR-27b-3p/VEGF-C axis modulates stemness characteristics in cervical cancer cells. Chin Med J (Engl) 134:2091-2101 (2021). PubMed: 34334630
- Xu C et al. Expression of OPN3 in lung adenocarcinoma promotes epithelial-mesenchymal transition and tumor metastasis. Thorac Cancer 11:286-294 (2020). PubMed: 31802643