Key features and details
- Rabbit polyclonal to OPN3 - N-terminal
- Suitable for: WB, IHC-P, IHC-Fr
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-OPN3 antibody - N-terminal
See all OPN3 primary antibodies
DescriptionRabbit polyclonal to OPN3 - N-terminal
Tested applicationsSuitable for: WB, IHC-P, IHC-Frmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide within Human OPN3 (N terminal). The exact sequence is proprietary. (Carrier-protein conjugated).
Database link: Q9H1Y3
- WB: A431 whole cell lysate. IHC-P: Human breast cancer tissue. IHC-Fr: Adult mouse retina tissue.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab228748 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 45 kDa.|
|IHC-P||1/100 - 1/1000.|
|IHC-Fr||1/100 - 1/1000.|
FunctionMay play a role in encephalic photoreception.
Tissue specificityStrongly expressed in brain. Highly expressed in the preoptic area and paraventricular nucleus of the hypothalamus. Shows highly patterned expression in other regions of the brain, being enriched in selected regions of the cerebral cortex, cerebellar Purkinje cells, a subset of striatal neurons, selected thalamic nuclei, and a subset of interneurons in the ventral horn of the spinal cord.
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family. Opsin subfamily.
- Information by UniProt
- ECPN antibody
- Encephalopsin antibody
- ERO antibody
Anti-OPN3 antibody - N-terminal (ab228748) at 1/1000 dilution + A431 (human epidermoid carcinoma cell line) whole cell lysate at 30 µg
Predicted band size: 45 kDa
10% SDS-PAGE gel.
Paraffin-embedded human breast cancer tissue stained for OPN3 using ab228748 at 1/250 dilution in immunohistochemical analysis.
Frozen sectioned adult mouse retina tissue stained for OPN3 using ab228748 at 1/250 dilution in immunohistochemical analysis (green).
The nuclear counterstain is DAPI (blue). Beta Tubulin 3 is detected with a beta Tubulin 3 antibody at 1/250 dilution (red).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab228748 has been referenced in 1 publication.
- Xu C et al. Expression of OPN3 in lung adenocarcinoma promotes epithelial-mesenchymal transition and tumor metastasis. Thorac Cancer 11:286-294 (2020). PubMed: 31802643