Key features and details
- Rabbit polyclonal to OPRT
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-OPRT antibody
See all OPRT primary antibodies
DescriptionRabbit polyclonal to OPRT
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow
Recombinant fragment corresponding to Human OPRT aa 217-466.
Database link: P11172
- HeLa and HCT116 whole cell lysates; HeLa cells; Human colon cancer tissue.
This product was previously labelled as UMPS
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab155763 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 52 kDa.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||1/100 - 1/1000.|
PathwayPyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 1/2.
Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 2/2.
Involvement in diseaseDefects in UMPS are the cause of orotic aciduria type 1 (ORAC1) [MIM:258900]. A disorder of pyrimidine metabolism resulting in megaloblastic anemia and orotic acid crystalluria that is frequently associated with some degree of physical and mental retardation. A minority of cases have additional features, particularly congenital malformations and immune deficiencies.
Sequence similaritiesIn the N-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.
In the C-terminal section; belongs to the OMP decarboxylase family.
- Information by UniProt
- ODC antibody
- OMPdecase antibody
- OPRT antibody
Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue labeling OPRT with ab155763 at 1/500 dilution.
Anti-OPRT antibody (ab155763) at 1/1000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 52 kDa
10% SDS PAGE
Immunofluorescence analysis of methanol-fixed HeLa cells labeling OPRT with ab155763 at 1/200 dilution (green). The image in the lower panel is costained with Hoechst 33342 (blue).
ab155763 has not yet been referenced specifically in any publications.