Overview

  • Product name

  • Description

    Rabbit polyclonal to OPRT
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant fragment corresponding to Human OPRT aa 217-466.
    Database link: P11172

  • Positive control

    • HeLa and HCT116 whole cell lysates; HeLa cells; Human colon cancer tissue.
  • General notes

     This product was previously labelled as UMPS

     

Properties

Applications

Our Abpromise guarantee covers the use of ab155763 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 52 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

  • Pathway

    Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 1/2.
    Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 2/2.
  • Involvement in disease

    Defects in UMPS are the cause of orotic aciduria type 1 (ORAC1) [MIM:258900]. A disorder of pyrimidine metabolism resulting in megaloblastic anemia and orotic acid crystalluria that is frequently associated with some degree of physical and mental retardation. A minority of cases have additional features, particularly congenital malformations and immune deficiencies.
  • Sequence similarities

    In the N-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.
    In the C-terminal section; belongs to the OMP decarboxylase family.
  • Information by UniProt
  • Database links

  • Alternative names

    • ODC antibody
    • OMPdecase antibody
    • OPRT antibody
    • OPRTase antibody
    • Orotate phosphoribosyl transferase and orotidine 5' decarboxylase antibody
    • Orotate phosphoribosyltransferase antibody
    • Orotate phosphoribosyltransferase phosphoribosyltransferase antibody
    • Orotidine 5' phosphate decarboxylase antibody
    • Orotidine 5''-phosphate decarboxylase antibody
    • RP11-71H17.9 antibody
    • UMP synthase antibody
    • Umps antibody
    • UMPS_HUMAN antibody
    • Uridine 5' monophosphate synthase antibody
    • Uridine monophosphate synthetase antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue labeling OPRT with ab155763 at 1/500 dilution.

  • Anti-OPRT antibody (ab155763) at 1/1000 dilution + HeLa whole cell lysate at 30 µg

    Predicted band size: 52 kDa



    10% SDS PAGE
  • Immunofluorescence analysis of methanol-fixed HeLa cells labeling OPRT with ab155763 at 1/200 dilution (green). The image in the lower panel is costained with Hoechst 33342 (blue).

References

ab155763 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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