Overview

  • Product name

    Optiblot Blue
  • General notes

    Optiblot Blue is a single step Coomassie based gel stain that is specially formulated for ultra-fast, sensitive and safe detection of your proteins. Protein gels can be stained in minutes without the need to wash, fix or destain. Only proteins are stained resulting in well defined blue bands on a highly transparent background. The reduction of background interference results in a better signal to noise ratio and may also have a positive impact on the overall resolution and sensitivity. The Optiblot Blue formulation is non-toxic and does not contain any methanol. Proteins stained using the Optiblot Blue stain are also compatible with mass spectrometry (MS) analysis.


    Manufactured under patent numbers : US 12/226,797 ; SG 201103010-3 ; SG 200807953-5 ; JP 2009-507182 ; IN 5722/CHENP/2008 ; GB 0608377.8 ; EP 10187154.9; EP 07733647.7 ; CN 200780021434.8 ; CA 2,649,835 ; AU 2007245443

Properties

Images

  • SDS-PAGE image showing molecular weight marker (MW) and His-tag recombinant protein fractions purified in Ni-NTA resin in lanes 1-5 stained with Optiblot Blue. Optiblot Blue was easy to handle and use - just pour a small amount onto your gel and within 10 minutes you can see the protein bands! No methanol and glacial acetic acid - no smell!

References

ab119211 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Answer


The loading control should have a different molecular weight than the target protein(s) that you are testing for. The two most common loading controls are beta actin and GAPDH. I've included links below to some of our most highly reviewed and referenced loading control antibodies:

Beta-actin
1. Mouse monoclonal https://www.abcam.com/beta-actin-antibody-mabcam-8226-loading-control-ab8226.html
2. Rabbit polyclonal https://www.abcam.com/beta-actin-antibody-loading-control-ab8227.html

GAPDH:
1. Mouse monoclonal https://www.abcam.com/gapdh-antibody-mabcam-9484-loading-control-ab9484.html
2. Rabbit polyclonal https://www.abcam.com/gapdh-antibody-ab9485.html

Our loading control guide, which notes specific conditions that may cause variance in the expression of these housekeeping genes, is given through the link below:

https://www.abcam.com/index.html?pageconfig=resource&rid=275

Thus, depending on the size of your target protein and your preference of clonality/host species any of these antibodies are a great choice, and are backed by our AbPromise guarantee to detect their respective target proteins in mouse via western blot.

We also carry our Optiblot line of western blot gels, buffers, and kits which offer strong signal and very low background for high sensitivity. Our Optiblot line is included through the link below:

Optiblot line: https://www.abcam.com/index.html?pageconfig=resource&rid=14709#3
Coomassie based gel stain: https://www.abcam.com/Optiblot-Blue-ab119211.html?refer404=catalogue%20119211

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Answer

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There is very little epitope information about any of the Von Willebrand Factor antibodies; because of this few have been identified as recognizing the N-terminus of vWF. Some of them may recognize the N-term, but they have not been epitope mapped. We would recommend use of a polyclonal antibody as these contain multiple epitope recognition sites and may include N-terminal sites. Ab6994 Rabbit polyclonal to Von Willebrand Factor would be a good choice for WB however whiles the product is known to work in a number of other applications this has not yet been tested in ELISA or IP.

Mouse monoclonal [VW92-3] to Von Willebrand Factor, ab129948 has been tested in ELISA and WB though not in IP. However this antibodies epitope is known to be V8 Protease Fragment III; N-termini Trypsin and Plasmin sensitive region.

Each product has been tested in some application but not others. For UNTESTED applications, we have established a testing discount program. Here is a brief description of how it works:

The testing discount program is for customers who like to use an antibody/protein on an untested species/application. You would purchase the antibody at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount equal to the value of the product tested. The terms and conditions of this offer can be found here: https://www.abcam.com/AbTrial

Abcam also provides a number of products for use in IP such as our https://www.abcam.com/ab119211 ready to use protein gel stain, our https://www.abcam.com/index.html?pageconfig=resource&rid=15115#1 and our https://www.abcam.com/index.html?pageconfig=resource&rid=15115#2 . Our VeriBlot line only recognize native (non-reduced) antibodies and are recommended when the target of interest is <30kDa, whereas our Light Chain specific line do not recognize heavy-chains and are recommended when your protein of interest is >30kDa. All of our products are covered by our six month Abpromise guarantee and are fully supported by our scientific support staff. More information about these products can be found at the links below.

Optiblot Blue: https://www.abcam.com/Optiblot-Blue-ab119211.html?refer404=catalogue%20119211

IP specific secondary antibodies: https://www.abcam.com/index.html?pageconfig=resource&rid=15115


I hope this information is helpful to you. Please do not hesitate to contact us if wish to enroll in our AbTrial program or if you need any more advice or information.

Free RabMab with any purchase of a primary antibody, while stocks last. Quote “RABMAB-XBSMG” in your next primary antibody order.

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Answer

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When using ab21899 Anti-beta 2 Microglobulin antibody [B2M-01] (Biotin) in IP we can recommend a working range of 1-10 ug/ml. The exact concentration for your IP experiments should be within this range but could be outside of this and will need to be determined experimentally.

Abcam also provides a number of products for use in IP such as our https://www.abcam.com/ab119211 ready to use protein gel stain, our https://www.abcam.com/index.html?pageconfig=resource&rid=15115#1 and our https://www.abcam.com/index.html?pageconfig=resource&rid=15115#2 . Our VeriBlot line only recognize native (non-reduced) antibodies and are recommended when the target of interest is <30kDa, whereas our Light Chain specific line do not recognize heavy-chains and are recommended when your protein of interest is >30kDa. All of our products are covered by our six month Abpromise guarantee and are fully supported by our scientific support staff. More information about these products can be found at the links below.

Optiblot Blue: https://www.abcam.com/Optiblot-Blue-ab119211.html?refer404=catalogue%20119211

IP specific secondary antibodies: https://www.abcam.com/index.html?pageconfig=resource&rid=15115


I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Answer

Thank you for contacting us.

Optiblot Blue is a G250 based stain.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Answer

Thank you for contacting us. OptiBlot Blue is a ready-to-use Coomassie stain that is optimal for obtaining well defined protein bands in a single step. It can be replaced by traditional Coomassie, and be used with the any electrophoretic gel. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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