Overview

  • Product name
    Optiblot ECL Detect Kit (23pg-187ng)
    See all ECL Substrate kits
  • Product overview

    Optiblot ECL Detect Kit (ab133406) is an enhanced chemiluminescent substrate specially developed for film imaging and is compatible with CCD imaging. Optiblot ECL Detect Kit produces a strong signal with very low background for high sensitivity. Additionally, the Optiblot ECL signal is long lasting, allowing repeated exposures without fear of losing data.

    200ml kit for 2000cm2 membrane

    500ml kit for 5000cm2 membrane

     

  • Notes

    Detection ranges stated should be used for guidance only as detection range is dependent on the molecular weight of a protein.

     

    Recommended primary antibody dilution range: 1/5000 - 1/20,000

    Recommended secondary antibody dilution range: 1/20,000 - 1/100,000

     

    *Due to sensitivity of substrate, optimisation may be required:

    Primary antibody concentration: start with usual primary antibody dilution and dilute 5 to 10 fold to find optimal conditions.

     

  • Tested applications
    Suitable for: WBmore details

Properties

Applications

Our Abpromise guarantee covers the use of ab133406 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration.

Images

  • Anti-Transferrin antibody (ab1223) at 1/25000 dilution. Lanes 1-14 Transferrin protein (ab91435), Loading dilution (Lanes 1-8): 12.5, 6.2, 3.1, 1.6, 0.8, 0.3, 0.19µg protein.

     

    Secondary

    HRP conjugated polyclonal to Rabbit IgG (ab97080) at 1/50000 developed using Optiblot ECL Detect and Vendor A ECL subtrate.

  • Each lane contains the following amount of COX2 recombinant protein (ab58868): 1) 100ng 2) 50ng 3) 25ng 4) 12.5ng 5) 6.25ng 6) 3.13ng 7) 1.56ng 8) 780pg 9) 390pg 10) 195pg 11) 98pg. The blot was probed with rabbit anti-COX2 antibody (ab15191) and with a rabbit secondary (ab97080). The blot was developed with 20ml of Optiblot ECL Detect.

  • Anti-COX2 antibody (ab15191) at 1/25000 dilution. Lanes 1-14 COX2 protein (ab58868), Loading dilution (Lanes 1-8): 12.5, 6.2, 3.1, 1.6, 0.8, 0.3, 0.19µg protein.

     

    Secondary:

    HRP conjugated polyclonal to Rabbit IgG (ab97080) at 1/50000 developed using Optiblot ECL Detect and Vendor A ECL subtrate.

  • Each lane contains the following amount of COX2 recombinant protein (ab58868): 1) 100ng 2) 50ng 3) 25ng 4) 12.5ng 5) 6.25ng 6) 3.13ng 7) 1.56ng 8) 780pg 9) 390pg 10) 195pg 11) 98pg. The blot was probed with rabbit anti-COX2 antibody (ab15191) and with a rabbit secondary (ab97080). The blot was developed with 20ml of Optiblot ECL Detect.

Protocols

References

This product has been referenced in:
  • Kostarnoy AV  et al. Receptor Mincle promotes skin allergies and is capable of recognizing cholesterol sulfate. Proc Natl Acad Sci U S A 114:E2758-E2765 (2017). WB . Read more (PubMed: 28292894) »
  • Hu F  et al. Ataxia-Telangiectasia Mutated (ATM) Protein Signaling Participates in Development of Pulmonary Arterial Hypertension in Rats. Med Sci Monit 23:4391-4400 (2017). Read more (PubMed: 28894083) »

See all 4 Publications for this product

Customer reviews and Q&As

Abreviews
The solution is highly sensitive, generating signal very fast (beta actin came in 20-30 sec, using the optiblot ECL detection solution developed in chem doc instrument).
Username

Abcam user community

Verified customer

Submitted Nov 09 2015

Visualization of Western blots

Good Good 4/5 (Ease of Use)
Abreviews
This abcam optiblot detect kit gives signal very fast like that of the west dura. For my protein of interest, Signal appeared between 50sec to 1.3mins in chemidoc, B-actin comes even faster (30-40sec). The concentration of the total protein loaded was 40ug).
Username

Abcam user community

Verified customer

Submitted Aug 07 2015

ab133409 has been used for ELISA applications.

However, since there are many different readers, plates, etc., testing/evaluation is necessary to assess utility for your specific experimental conditions.

Thank you for your enquiry.

I am sorry to confirm that ab133406 is not suitable for detection of AP. This detection system has been designed for optimal detection of HRP, and will not be as sensitive for detection of AP.

I am sorr...

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Thank you for contacting us.

The alternative we have is ab133406; https://www.abcam.com/Optiblot-ECL-Detect-Kit-23pg-187ng-ab133406.html

I hope this information is helpful to you. Please do not hesitate to contact us if you need any m...

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Thank you for contacting us. I hope that you are well.

The ab133410 is a fluorescence based kit however the Roche kit cat # 11520709001 is a chemiluminescence based so this will not be a right alternative.

Please suggest ab133406, htt...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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