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Optiblot ECL Detect Kit (ab133406) is an enhanced chemiluminescent substrate specially developed for film imaging and is compatible with CCD imaging. Optiblot ECL Detect Kit produces a strong signal with very low background for high sensitivity. Additionally, the Optiblot ECL signal is long lasting, allowing repeated exposures without fear of losing data.
200ml kit for 2000cm2 membrane
500ml kit for 5000cm2 membrane
Detection ranges stated should be used for guidance only as detection range is dependent on the molecular weight of a protein.
Recommended primary antibody dilution range: 1/5000 - 1/20,000
Recommended secondary antibody dilution range: 1/20,000 - 1/100,000
*Due to sensitivity of substrate, optimisation may be required:
Primary antibody concentration: start with usual primary antibody dilution and dilute 5 to 10 fold to find optimal conditions.
|Components||200 ml||20 ml||500 ml|
|Luminol/Enhancer Solution||1 x 100ml||1 x 10ml||1 x 250ml|
|Peroxide Chemiluminescent Detection Reagent||1 x 100ml||1 x 10ml||1 x 250ml|
Our Abpromise guarantee covers the use of ab133406 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration.|
HRP conjugated polyclonal to Rabbit IgG (ab97080) at 1/50000 developed using Optiblot ECL Detect and Vendor A ECL subtrate.
Each lane contains the following amount of COX2 recombinant protein (ab58868): 1) 100ng 2) 50ng 3) 25ng 4) 12.5ng 5) 6.25ng 6) 3.13ng 7) 1.56ng 8) 780pg 9) 390pg 10) 195pg 11) 98pg. The blot was probed with rabbit anti-COX2 antibody (ab15191) and with a rabbit secondary (ab97080). The blot was developed with 20ml of Optiblot ECL Detect.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"