Overview

  • Product name

    Optiblot LDS Sample Buffer (4X)
    See all Western blot buffer reagents
  • Tested applications

    Suitable for: SDS-PAGE, WBmore details
  • General notes

    Optiblot LDS Sample Buffer should be used to prepare all samples for runing on Optiblot Precast Gels. Specifically formulated for the Optiblot buffer system to provide the best running conditions and results.

    Optiblot gels must be used with Optiblot buffers

    Buffer recipes are available in the protocol tab.

Properties

Applications

Our Abpromise guarantee covers the use of ab119196 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
SDS-PAGE Use at an assay dependent concentration.
WB Use at an assay dependent concentration.

References

ab119196 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Excellent Sample buffer

Excellent Excellent 5/5 (Ease of Use)
Abreviews
The sample buffer was used to prepare human prostate tissue lysate for western botting. A clear separation was able to achieve for the detecting the target antibody.

Abcam user community

Verified customer

Submitted Dec 28 2016

Excellent item

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Excellent item, there is a very nice red line to help you out with the migration. very cheap

Dr. Fabrice Gankamk

Verified customer

Submitted Dec 08 2014

Answer

Thank you for your reply.
I'd suggest using the sample buffer that is compatible with the gel, namely ab119196.
https://www.abcam.com/optiblot-lds-sample-buffer-4x-ab119196.html
This is what is recommended to be used.
I can doublecheck this with the lab, but I don't think the standard Laemmli buffer would give you good results. I'll let you know.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
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