Product nameOptiblot SDS-PAGE Sample Preparation Kit
The Optiblot SDS-PAGE Sample Preparation Kit (ab133414) provides a means to quickly concentrate protein samples, and separate them from buffers that interfere with electrophoresis.
The fast, efficient protocol generates samples ready to load on a gel in less than ten minutes, much more quickly than can be achieved with alternate methods such as dialysis, or acetone or TCA precipitation. Additionally, the Optiblot SDS-PAGE Sample Preparation protocol is easily scaled up, allowing multiple samples to be prepared in parallel.
Tested applicationsSuitable for: Purificationmore details
Components 10 tests 25 tests Extraction loading buffer 1 x 1ml 1 x 1ml Protein filters 10 units 25 units Resin 1 x 500ml 2 x 500ml
- Prestained Protein Ladder - Mid-range molecular weight (10-175 kDa) (ab115832)
- Prestained Protein Ladder - Mid-range molecular weight (10 - 180 kDa) (ab116027)
- Prestained Protein Ladder - Broad molecular weight (10-245 kDa) (ab116028)
- Precast Gel SDS-PAGE 4-20% (8x10cm,12well) (ab119209)
- Optiblot Blue (ab119211)
- Bradford Reagent (ab119216)
- Chemiluminescent HRP Substrate (membrane) (ab5801)
Our Abpromise guarantee covers the use of ab133414 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Purification||Use at an assay dependent concentration.|
Resin concentration is compatible with Western blot detection. Western blots were created with samples of A431 cell extract (panels a and b, lane 2), diluted cell extract (lane 3) and the diluted extract concentrated using the Optiblot SDS-PAGE Sample Preparation Kit protocol (lane 4). The blots were detected using the Optiblot Fluorescent Western Blot Kit (ab133410). No bands were detectable in the diluted cell extract when stained for either GAPDH (panel a, lane 3) or SRC (panel b, lane 3). However, after resin concentration, bands are easily visualized for both proteins (lanes 4). Band intensities are indicated.
ab133414 has not yet been referenced specifically in any publications.