Anti-Optineurin antibody (ab23666)
Key features and details
- Rabbit polyclonal to Optineurin
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-Optineurin antibody
See all Optineurin primary antibodies -
Description
Rabbit polyclonal to Optineurin -
Host species
Rabbit -
Tested Applications & Species
Application Species WB Human -
Immunogen
Synthetic peptide. This information is considered to be commercially sensitive.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituents: 66% Sodium chloride, 25% Tris -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab23666 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
---|---|
WB |
Human
|
All applications |
Mouse
Rat
Cow
Pig
Cynomolgus monkey
Macaque monkey
|
Application | Abreviews | Notes |
---|---|---|
WB | (3) |
1/200. Detects a band of approximately 75 kDa (predicted molecular weight: 74 kDa).
|
Notes |
---|
WB
1/200. Detects a band of approximately 75 kDa (predicted molecular weight: 74 kDa). |
Target
-
Function
Plays an important role in the maintenance of the Golgi complex, in membrane trafficking, in exocytosis, through its interaction with myosin VI and Rab8. Links myosin VI to the Golgi complex and plays an important role in Golgi ribbon formation. Negatively regulates the induction of IFNB in response to RNA virus infection. Plays a neuroprotective role in the eye and optic nerve. Probably part of the TNF-alpha signaling pathway that can shift the equilibrium toward induction of cell death. May act by regulating membrane trafficking and cellular morphogenesis via a complex that contains Rab8 and hungtingtin (HD). May constitute a cellular target for adenovirus E3 14.7, an inhibitor of TNF-alpha functions, thereby affecting cell death. -
Tissue specificity
Present in acqueous humor of the eye (at protein level). Highly expressed in trabecular meshwork. Expressed nonpigmented ciliary epithelium, retina, brain, adrenal cortex, fetus, lymphocyte, fibroblast, skeletal muscle, heart, liver, brain and placenta. -
Involvement in disease
Defects in OPTN are the cause of primary open angle glaucoma type 1E (GLC1E) [MIM:137760]. Primary open angle glaucoma (POAG) is characterized by a specific pattern of optic nerve and visual field defects. The angle of the anterior chamber of the eye is open, and usually the intraocular pressure is increased. The disease is asymptomatic until the late stages, by which time significant and irreversible optic nerve damage has already taken place.
Defects in OPTN are a cause of susceptibility to normal pressure glaucoma (NPG) [MIM:606657].
Defects in OPTN are the cause of amyotrophic lateral sclerosis type 12 (ALS12) [MIM:613435]. It is a neurodegenerative disorder affecting upper motor neurons in the brain and lower motor neurons in the brain stem and spinal cord, resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10% of the cases. -
Domain
Ubiquitin-binding motif (UBAN) is essential for its inhibitory function, subcellular localization and interaction with TBK1. -
Post-translational
modificationsPhosphorylated. Phosphorylation is induced by phorbol esters and decreases its half-time. -
Cellular localization
Cytoplasm > perinuclear region. Golgi apparatus. Golgi apparatus > trans-Golgi network. Found in the perinuclear region and associates with the Golgi apparatus. Colocalizes with MYO6 and RAB8 at the Golgi complex and in vesicular structures close to the plasma membrane. - Information by UniProt
-
Database links
- Entrez Gene: 10133 Human
- Entrez Gene: 71648 Mouse
- Entrez Gene: 397011 Pig
- Entrez Gene: 246294 Rat
- Omim: 602432 Human
- SwissProt: Q95KA2 Cynomolgus monkey
- SwissProt: Q96CV9 Human
- SwissProt: Q8K3K8 Mouse
see all -
Alternative names
- 14.7K interacting protein antibody
- Ag9 C5 antibody
- ALS12 antibody
see all
Images
-
Anti-Optineurin antibody (ab23666) at 1 µg + Human skeletal muscle tissue lysate - total protein at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 74 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Additional bands at: 24 kDa, 43 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minute
Datasheets and documents
-
SDS download
-
Datasheet download
References (28)
ab23666 has been referenced in 28 publications.
- Putyrski M et al. Disrupting the LC3 Interaction Region (LIR) Binding of Selective Autophagy Receptors Sensitizes AML Cell Lines to Cytarabine. Front Cell Dev Biol 8:208 (2020). PubMed: 32296703
- Shi L et al. SIRT5-mediated deacetylation of LDHB promotes autophagy and tumorigenesis in colorectal cancer. Mol Oncol 13:358-375 (2019). PubMed: 30443978
- Bordi M et al. mTOR hyperactivation in Down Syndrome underlies deficits in autophagy induction, autophagosome formation, and mitophagy. Cell Death Dis 10:563 (2019). PubMed: 31332166
- Heo JM et al. Integrated proteogenetic analysis reveals the landscape of a mitochondrial-autophagosome synapse during PARK2-dependent mitophagy. Sci Adv 5:eaay4624 (2019). PubMed: 31723608
- Moharir SC et al. Identification of a splice variant of optineurin which is defective in autophagy and phosphorylation. Biochim Biophys Acta Mol Cell Res 1865:1526-1538 (2018). PubMed: 30327196