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    optineurin-antibody-ab23666.pdf

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Immunology Innate Immunity Cytokines TNF Superfamily
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Anti-Optineurin antibody (ab23666)

  • Datasheet
  • SDS
Reviews (5) Submit a question References (28)

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Western blot - Anti-Optineurin antibody (ab23666)

    Key features and details

    • Rabbit polyclonal to Optineurin
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

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    Protein
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    Recombinant Human Optineurin protein (His tag) (ab235021)

    View more associated products

    Overview

    • Product name

      Anti-Optineurin antibody
      See all Optineurin primary antibodies
    • Description

      Rabbit polyclonal to Optineurin
    • Host species

      Rabbit
    • Tested Applications & Species

      Application Species
      WB
      Human
      See all applications and species data
    • Immunogen

      Synthetic peptide. This information is considered to be commercially sensitive.

    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

      One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

      Learn more here.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
    • Storage buffer

      pH: 7.40
      Constituents: 66% Sodium chloride, 25% Tris
    • Concentration information loading...
    • Purity

      Protein A purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Immunology
      • Innate Immunity
      • Cytokines
      • TNF Superfamily
      • Signal Transduction
      • Adapters
      • Cytoplasmic
      • Neuroscience
      • Sensory System
      • Visual system

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant Human Optineurin protein (His tag) (ab235021)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab23666 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    Human
    All applications
    Mouse
    Rat
    Cow
    Pig
    Cynomolgus monkey
    Macaque monkey
    Application Abreviews Notes
    WB (3)
    1/200. Detects a band of approximately 75 kDa (predicted molecular weight: 74 kDa).
    Notes
    WB
    1/200. Detects a band of approximately 75 kDa (predicted molecular weight: 74 kDa).

    Target

    • Function

      Plays an important role in the maintenance of the Golgi complex, in membrane trafficking, in exocytosis, through its interaction with myosin VI and Rab8. Links myosin VI to the Golgi complex and plays an important role in Golgi ribbon formation. Negatively regulates the induction of IFNB in response to RNA virus infection. Plays a neuroprotective role in the eye and optic nerve. Probably part of the TNF-alpha signaling pathway that can shift the equilibrium toward induction of cell death. May act by regulating membrane trafficking and cellular morphogenesis via a complex that contains Rab8 and hungtingtin (HD). May constitute a cellular target for adenovirus E3 14.7, an inhibitor of TNF-alpha functions, thereby affecting cell death.
    • Tissue specificity

      Present in acqueous humor of the eye (at protein level). Highly expressed in trabecular meshwork. Expressed nonpigmented ciliary epithelium, retina, brain, adrenal cortex, fetus, lymphocyte, fibroblast, skeletal muscle, heart, liver, brain and placenta.
    • Involvement in disease

      Defects in OPTN are the cause of primary open angle glaucoma type 1E (GLC1E) [MIM:137760]. Primary open angle glaucoma (POAG) is characterized by a specific pattern of optic nerve and visual field defects. The angle of the anterior chamber of the eye is open, and usually the intraocular pressure is increased. The disease is asymptomatic until the late stages, by which time significant and irreversible optic nerve damage has already taken place.
      Defects in OPTN are a cause of susceptibility to normal pressure glaucoma (NPG) [MIM:606657].
      Defects in OPTN are the cause of amyotrophic lateral sclerosis type 12 (ALS12) [MIM:613435]. It is a neurodegenerative disorder affecting upper motor neurons in the brain and lower motor neurons in the brain stem and spinal cord, resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10% of the cases.
    • Domain

      Ubiquitin-binding motif (UBAN) is essential for its inhibitory function, subcellular localization and interaction with TBK1.
    • Post-translational
      modifications

      Phosphorylated. Phosphorylation is induced by phorbol esters and decreases its half-time.
    • Cellular localization

      Cytoplasm > perinuclear region. Golgi apparatus. Golgi apparatus > trans-Golgi network. Found in the perinuclear region and associates with the Golgi apparatus. Colocalizes with MYO6 and RAB8 at the Golgi complex and in vesicular structures close to the plasma membrane.
    • Target information above from: UniProt accession Q96CV9 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 10133 Human
      • Entrez Gene: 71648 Mouse
      • Entrez Gene: 397011 Pig
      • Entrez Gene: 246294 Rat
      • Omim: 602432 Human
      • SwissProt: Q95KA2 Cynomolgus monkey
      • SwissProt: Q96CV9 Human
      • SwissProt: Q8K3K8 Mouse
      • SwissProt: Q7YS99 Pig
      • SwissProt: Q8R5M4 Rat
      • Unigene: 332706 Human
      • Unigene: 116942 Mouse
      • Unigene: 63435 Rat
      see all
    • Alternative names

      • 14.7K interacting protein antibody
      • Ag9 C5 antibody
      • ALS12 antibody
      • E3 14.7K interacting protein antibody
      • E3-14.7K-interacting protein antibody
      • FIP 2 antibody
      • FIP-2 antibody
      • FIP2 antibody
      • Glaucoma 1 open angle E (adult onset) antibody
      • Glaucoma 1 open angle E antibody
      • GLC1E antibody
      • HIP 7 antibody
      • HIP-7 antibody
      • HIP7 antibody
      • Huntingtin interacting protein 7 antibody
      • Huntingtin interacting protein HYPL antibody
      • Huntingtin interacting protein L antibody
      • Huntingtin yeast partner L antibody
      • Huntingtin-interacting protein 7 antibody
      • Huntingtin-interacting protein L antibody
      • HYPL antibody
      • Injury inducible protein I 55 antibody
      • NEMO related protein antibody
      • NEMO-related protein antibody
      • NRP antibody
      • Optic neuropathy inducing protein antibody
      • Optic neuropathy-inducing protein antibody
      • Optineurin antibody
      • OPTN antibody
      • OPTN_HUMAN antibody
      • TFIIIA IntP antibody
      • TFIIIA-IntP antibody
      • Transcription factor IIIA interacting protein antibody
      • Transcription factor IIIA-interacting protein antibody
      • Tumor necrosis factor alpha inducible cellular protein containing leucine zipper domains antibody
      see all

    Images

    • Western blot - Anti-Optineurin antibody (ab23666)
      Western blot - Anti-Optineurin antibody (ab23666)
      Anti-Optineurin antibody (ab23666) at 1 µg + Human skeletal muscle tissue lysate - total protein at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 74 kDa
      Observed band size: 75 kDa why is the actual band size different from the predicted?
      Additional bands at: 24 kDa, 43 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 1 minute

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (28)

    Publishing research using ab23666? Please let us know so that we can cite the reference in this datasheet.

    ab23666 has been referenced in 28 publications.

    • Putyrski M  et al. Disrupting the LC3 Interaction Region (LIR) Binding of Selective Autophagy Receptors Sensitizes AML Cell Lines to Cytarabine. Front Cell Dev Biol 8:208 (2020). PubMed: 32296703
    • Shi L  et al. SIRT5-mediated deacetylation of LDHB promotes autophagy and tumorigenesis in colorectal cancer. Mol Oncol 13:358-375 (2019). PubMed: 30443978
    • Bordi M  et al. mTOR hyperactivation in Down Syndrome underlies deficits in autophagy induction, autophagosome formation, and mitophagy. Cell Death Dis 10:563 (2019). PubMed: 31332166
    • Heo JM  et al. Integrated proteogenetic analysis reveals the landscape of a mitochondrial-autophagosome synapse during PARK2-dependent mitophagy. Sci Adv 5:eaay4624 (2019). PubMed: 31723608
    • Moharir SC  et al. Identification of a splice variant of optineurin which is defective in autophagy and phosphorylation. Biochim Biophys Acta Mol Cell Res 1865:1526-1538 (2018). PubMed: 30327196
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-5 of 5 Abreviews or Q&A

    Western blot abreview for Anti-Optineurin antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Dopaminergic Neurons)
    Gel Running Conditions
    Reduced Denaturing (NuPage 4-12% MES buffer)
    Loading amount
    30 µg
    Specification
    Dopaminergic Neurons
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 22 2020

    Western blot abreview for Anti-Optineurin antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HEK293T, HCT116)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    30 µg
    Specification
    HEK293T, HCT116
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 28 2019

    Immunohistochemistry (Frozen sections) abreview for Anti-Optineurin antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    fish gelantine and ovalbumine as blocking agent for 30 minute(s) · Concentration: 0.6% · Temperature: 22°C
    Sample
    Mouse Tissue sections (Retina)
    Specification
    Retina
    Permeabilization
    Yes - PBS-Tween 0.01%
    Fixative
    only frozen
    Read More

    Abcam user community

    Verified customer

    Submitted Jul 23 2013

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Optineurin antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cultured Cells (Primary hippocampal neurons)
    Permeabilization
    Yes - 0,25% Triton in PBS
    Specification
    Primary hippocampal neurons
    Blocking step
    Donkey Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 14 2011

    Western blot abreview for Anti-Optineurin antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Tissue lysate - other (brain lysate differentially centrifuged)
    Loading amount
    5 µg
    Specification
    brain lysate differentially centrifuged
    Gel Running Conditions
    Non-reduced Denaturing (12 % SDS Gel)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 04 2011

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