Recombinant
RabMAb

Recombinant Anti-Optineurin antibody [EPR23059-124] (ab242146)

Overview

  • Product name

    Anti-Optineurin antibody [EPR23059-124]
    See all Optineurin primary antibodies
  • Description

    Rabbit monoclonal [EPR23059-124] to Optineurin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details
    Unsuitable for: ICC/IF or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human Optineurin aa 250 to the C-terminus. The exact sequence is proprietary.
    Database link: Q96CV9

  • Positive control

    • WB: Human brain and Hela lysates. IHC-P: Human cerebrum tissue. FC: HeLa cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab242146 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 66 kDa.

 

 

IHC-P 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

 

 

Flow Cyt 1/50.

 

 

  • Application notes
    Is unsuitable for ICC/IF or IP.
  • Target

    • Function

      Plays an important role in the maintenance of the Golgi complex, in membrane trafficking, in exocytosis, through its interaction with myosin VI and Rab8. Links myosin VI to the Golgi complex and plays an important role in Golgi ribbon formation. Negatively regulates the induction of IFNB in response to RNA virus infection. Plays a neuroprotective role in the eye and optic nerve. Probably part of the TNF-alpha signaling pathway that can shift the equilibrium toward induction of cell death. May act by regulating membrane trafficking and cellular morphogenesis via a complex that contains Rab8 and hungtingtin (HD). May constitute a cellular target for adenovirus E3 14.7, an inhibitor of TNF-alpha functions, thereby affecting cell death.
    • Tissue specificity

      Present in acqueous humor of the eye (at protein level). Highly expressed in trabecular meshwork. Expressed nonpigmented ciliary epithelium, retina, brain, adrenal cortex, fetus, lymphocyte, fibroblast, skeletal muscle, heart, liver, brain and placenta.
    • Involvement in disease

      Defects in OPTN are the cause of primary open angle glaucoma type 1E (GLC1E) [MIM:137760]. Primary open angle glaucoma (POAG) is characterized by a specific pattern of optic nerve and visual field defects. The angle of the anterior chamber of the eye is open, and usually the intraocular pressure is increased. The disease is asymptomatic until the late stages, by which time significant and irreversible optic nerve damage has already taken place.
      Defects in OPTN are a cause of susceptibility to normal pressure glaucoma (NPG) [MIM:606657].
      Defects in OPTN are the cause of amyotrophic lateral sclerosis type 12 (ALS12) [MIM:613435]. It is a neurodegenerative disorder affecting upper motor neurons in the brain and lower motor neurons in the brain stem and spinal cord, resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10% of the cases.
    • Domain

      Ubiquitin-binding motif (UBAN) is essential for its inhibitory function, subcellular localization and interaction with TBK1.
    • Post-translational
      modifications

      Phosphorylated. Phosphorylation is induced by phorbol esters and decreases its half-time.
    • Cellular localization

      Cytoplasm > perinuclear region. Golgi apparatus. Golgi apparatus > trans-Golgi network. Found in the perinuclear region and associates with the Golgi apparatus. Colocalizes with MYO6 and RAB8 at the Golgi complex and in vesicular structures close to the plasma membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • 14.7K interacting protein antibody
      • Ag9 C5 antibody
      • ALS12 antibody
      • E3 14.7K interacting protein antibody
      • E3-14.7K-interacting protein antibody
      • FIP 2 antibody
      • FIP-2 antibody
      • FIP2 antibody
      • Glaucoma 1 open angle E (adult onset) antibody
      • Glaucoma 1 open angle E antibody
      • GLC1E antibody
      • HIP 7 antibody
      • HIP-7 antibody
      • HIP7 antibody
      • Huntingtin interacting protein 7 antibody
      • Huntingtin interacting protein HYPL antibody
      • Huntingtin interacting protein L antibody
      • Huntingtin yeast partner L antibody
      • Huntingtin-interacting protein 7 antibody
      • Huntingtin-interacting protein L antibody
      • HYPL antibody
      • Injury inducible protein I 55 antibody
      • NEMO related protein antibody
      • NEMO-related protein antibody
      • NRP antibody
      • Optic neuropathy inducing protein antibody
      • Optic neuropathy-inducing protein antibody
      • Optineurin antibody
      • OPTN antibody
      • OPTN_HUMAN antibody
      • TFIIIA IntP antibody
      • TFIIIA-IntP antibody
      • Transcription factor IIIA interacting protein antibody
      • Transcription factor IIIA-interacting protein antibody
      • Tumor necrosis factor alpha inducible cellular protein containing leucine zipper domains antibody
      see all

    Images

    • All lanes : Anti-Optineurin antibody [EPR23059-124] (ab242146) at 1/1000 dilution

      Lane 1 : Human brain lysate
      Lane 2 : Hela (human cervix adenocarcinoma epithelial cell) lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 66 kDa
      Observed band size: 74 kDa
      why is the actual band size different from the predicted?



      Blocking and diluting buffer and concentration: 5% NFDM/TBST The molecular weight observed is consistent with what has been described in the literature (PMID: 27620379). 

      Exposure time: Lane 1: 15 seconds Lane 2: 70 seconds

    • Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Optineurin with ab242146 at 1/500 dilution (0.98 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on human cerebrum. The section was incubated with ab242146 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND®RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.

    • Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervix adenocarcinoma) cells labelling Optineurin with ab242146 at 1/50 (Red) compared with a Rabbit monoclonal IgG (ab172730, Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor®488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    References

    ab242146 has not yet been referenced specifically in any publications.

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