• Product name
  • Description
    Rabbit polyclonal to Orai1
  • Host species
  • Specificity
    ab59330 is predicted to have no cross-reactivity to Orai2 or Orai3.
  • Tested applications
    Suitable for: ICC/IF, Flow Cyt, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    An 18 amino acid peptide from near the amino terminus of human Orai1.

  • Positive control
    • Human ovary tissue lysate and human spleen tissue.



Our Abpromise guarantee covers the use of ab59330 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 21632714
Flow Cyt Use at an assay dependent concentration. PubMed: 19965684

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.


IHC-P Use a concentration of 10 µg/ml.
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 33 kDa).


  • Function
    Ca(2+) release-activated Ca(2+) (CRAC) channel subunit which mediates Ca(2+) influx following depletion of intracellular Ca(2+) stores and channel activation by the Ca(2+) sensor, STIM1. CRAC channels are the main pathway for Ca(2+) influx in T-cells and promote the immune response to pathogens by activating the transcription factor NFAT.
  • Involvement in disease
    Defects in ORAI1 are the cause of immune dysfunction with T-cell inactivation due to calcium entry defect type 1 (IDTICED1) [MIM:612782]. IDTICED1 is an immune disorder characterized by recurrent infections, impaired T-cell activation and proliferative response, decreased T-cell production of cytokines, and normal lymphocytes counts and serum immunoglobulin levels. In surviving patients ectodermal dysplasia with anhydrosis and non-progressive myopathy may be observed.
  • Sequence similarities
    Belongs to the Orai family.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Calcium release activated calcium channel protein 1 antibody
    • Calcium release activated calcium modulator 1 antibody
    • Calcium release-activated calcium channel protein 1 antibody
    • CRACM 1 antibody
    • CRACM1 antibody
    • CRCM1 antibody
    • CRCM1_HUMAN antibody
    • FLJ14466 antibody
    • Orai 1 antibody
    • ORAI calcium release activated calcium modulator 1 antibody
    • orai1 antibody
    • ORAT1 antibody
    • Protein orai 1 antibody
    • Protein orai-1 antibody
    • TMEM 142A antibody
    • TMEM142A antibody
    • Transmembrane protein 142A antibody
    see all


  • All lanes : Anti-Orai1 antibody (ab59330) at 1 µg/ml

    Lane 1 : human ovary tissue lysate with no blocking peptide
    Lane 2 : human ovary tissue lysate with blocking peptide

    Lysates/proteins at 15 µg per lane.

    Predicted band size: 33 kDa
    Observed band size: 50 kDa (why is the actual band size different from the predicted?)

    Orai1 often migrates at a higher than expected molecular weight in SDS-PAGE.
  • ab59330 at 10ug/ml staining Orai1 in human spleen by Immunohistochemistry.


This product has been referenced in:
  • Hofhuis J  et al. Dysferlin mediates membrane tubulation and links T-tubule biogenesis to muscular dystrophy. J Cell Sci 130:841-852 (2017). Read more (PubMed: 28104817) »
  • Huang M  et al. The maintenance ability and Ca(2+) availability of skeletal muscle are enhanced by sildenafil. Exp Mol Med 48:e278 (2016). WB . Read more (PubMed: 27932789) »

See all 10 Publications for this product

Customer reviews and Q&As

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