Recombinant Anti-Orai1 antibody [EPR3394(2)] (ab111960)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-Orai1 antibody [EPR3394(2)]
See all Orai1 primary antibodies -
Description
Rabbit monoclonal [EPR3394(2)] to Orai1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: ICC,IHC-Fr,IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide, corresponding to residues from Human Orai1.
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Positive control
- Human platelet lysate and Ovary cancer lysate.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR3394(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab111960 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 35,50 kDa (predicted molecular weight: 33 kDa). The 50kda band is the glycosylated Orai1 protein (PMID: 26956484). |
Target
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Function
Ca(2+) release-activated Ca(2+) (CRAC) channel subunit which mediates Ca(2+) influx following depletion of intracellular Ca(2+) stores and channel activation by the Ca(2+) sensor, STIM1. CRAC channels are the main pathway for Ca(2+) influx in T-cells and promote the immune response to pathogens by activating the transcription factor NFAT. -
Involvement in disease
Defects in ORAI1 are the cause of immune dysfunction with T-cell inactivation due to calcium entry defect type 1 (IDTICED1) [MIM:612782]. IDTICED1 is an immune disorder characterized by recurrent infections, impaired T-cell activation and proliferative response, decreased T-cell production of cytokines, and normal lymphocytes counts and serum immunoglobulin levels. In surviving patients ectodermal dysplasia with anhydrosis and non-progressive myopathy may be observed. -
Sequence similarities
Belongs to the Orai family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 84876 Human
- Omim: 610277 Human
- SwissProt: Q96D31 Human
- Unigene: 55148 Human
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Alternative names
- Calcium release activated calcium channel protein 1 antibody
- Calcium release activated calcium modulator 1 antibody
- Calcium release-activated calcium channel protein 1 antibody
see all
Images
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![Western blot - Anti-Orai1 antibody [EPR3394(2)] (ab111960)](https://www.abcam.com/ps/products/111/ab111960/Images/ab111960-125216-anti-orai1-antibody-epr33942-western-blot.jpg)
Western blot - Anti-Orai1 antibody [EPR3394(2)] (ab111960)All lanes : Anti-Orai1 antibody [EPR3394(2)] (ab111960) at 1/1000 dilution
Lane 1 : Human platelet lysate
Lane 2 : Human ovary cancer lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 33 kDaThe 50kda band is the glycosylated Orai1 protein (PMID: 26956484).
Protocols
Datasheets and documents
References
This product has been referenced in:
- Zhu M et al. Store-operated Ca(2+) entry regulates glioma cell migration and invasion via modulation of Pyk2 phosphorylation. J Exp Clin Cancer Res 33:98 (2014). Read more (PubMed: 25433371) »
