Recombinant Anti-Orai1 antibody [EPR3394(2)] (ab111960)
![Western blot - Anti-Orai1 antibody [EPR3394(2)] (ab111960)](https://www.abcam.com/ps/products/111/ab111960/Images/ab111960-125216-anti-orai1-antibody-epr33942-western-blot.jpg "Western blot - Anti-Orai1 antibody [EPR3394(2)] (ab111960)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3394(2)] to Orai1
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-Orai1 antibody [EPR3394(2)]
See all Orai1 primary antibodies -
Description
Rabbit monoclonal [EPR3394(2)] to Orai1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: ICC,IHC-Fr,IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide, corresponding to residues from Human Orai1.
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Positive control
- Human platelet lysate and Ovary cancer lysate.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR3394(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab111960 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 35,50 kDa (predicted molecular weight: 33 kDa). The 50kda band is the glycosylated Orai1 protein (PMID: 26956484). |
Target
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Function
Ca(2+) release-activated Ca(2+) (CRAC) channel subunit which mediates Ca(2+) influx following depletion of intracellular Ca(2+) stores and channel activation by the Ca(2+) sensor, STIM1. CRAC channels are the main pathway for Ca(2+) influx in T-cells and promote the immune response to pathogens by activating the transcription factor NFAT. -
Involvement in disease
Defects in ORAI1 are the cause of immune dysfunction with T-cell inactivation due to calcium entry defect type 1 (IDTICED1) [MIM:612782]. IDTICED1 is an immune disorder characterized by recurrent infections, impaired T-cell activation and proliferative response, decreased T-cell production of cytokines, and normal lymphocytes counts and serum immunoglobulin levels. In surviving patients ectodermal dysplasia with anhydrosis and non-progressive myopathy may be observed. -
Sequence similarities
Belongs to the Orai family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 84876 Human
- Omim: 610277 Human
- SwissProt: Q96D31 Human
- Unigene: 55148 Human
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Alternative names
- Calcium release activated calcium channel protein 1 antibody
- Calcium release activated calcium modulator 1 antibody
- Calcium release-activated calcium channel protein 1 antibody
see all
Images
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Western blot - Anti-Orai1 antibody [EPR3394(2)] (ab111960)All lanes : Anti-Orai1 antibody [EPR3394(2)] (ab111960) at 1/1000 dilution
Lane 1 : Human platelet lysate
Lane 2 : Human ovary cancer lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 33 kDaThe 50kda band is the glycosylated Orai1 protein (PMID: 26956484).
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![Anti-Orai1 antibody [EPR3394(2)] (ab111960)](https://www.abcam.com/ps/products/111/ab111960/Images/ab111960-2-benefits-of-recombinant-antibodies.png)
Anti-Orai1 antibody [EPR3394(2)] (ab111960)
References (1)
ab111960 has been referenced in 1 publication.
- Zhu M et al. Store-operated Ca(2+) entry regulates glioma cell migration and invasion via modulation of Pyk2 phosphorylation. J Exp Clin Cancer Res 33:98 (2014). PubMed: 25433371
