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    orai1-antibody-epr33942-ab111960.pdf

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Immunology Adaptive Immunity T Cells Non-CD
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RecombinantRabMAb

Recombinant Anti-Orai1 antibody [EPR3394(2)] (ab111960)

  • Datasheet
  • SDS
Submit a review Q&A (2)References (1)

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Western blot - Anti-Orai1 antibody [EPR3394(2)] (ab111960)
  • Anti-Orai1 antibody [EPR3394(2)] (ab111960)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3394(2)] to Orai1
  • Suitable for: WB
  • Reacts with: Human

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Overview

  • Product name

    Anti-Orai1 antibody [EPR3394(2)]
    See all Orai1 primary antibodies
  • Description

    Rabbit monoclonal [EPR3394(2)] to Orai1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: ICC,IHC-Fr,IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to residues from Human Orai1.

  • Positive control

    • Human platelet lysate and Ovary cancer lysate.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR3394(2)
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Adaptive Immunity
    • T Cells
    • Non-CD
    • Signal Transduction
    • Signaling Pathway
    • Calcium Signaling
    • Calcium Channels

Associated products

  • Alternative Versions

    • Anti-Orai1 antibody [EPR3394(2)] - BSA and Azide free (ab226081)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

Applications

Our Abpromise guarantee covers the use of ab111960 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 35,50 kDa (predicted molecular weight: 33 kDa).

The 50kda band is the glycosylated Orai1 protein (PMID: 26956484).

  • Application notes
    Is unsuitable for ICC,IHC-Fr,IHC-P or IP.
  • Target

    • Function

      Ca(2+) release-activated Ca(2+) (CRAC) channel subunit which mediates Ca(2+) influx following depletion of intracellular Ca(2+) stores and channel activation by the Ca(2+) sensor, STIM1. CRAC channels are the main pathway for Ca(2+) influx in T-cells and promote the immune response to pathogens by activating the transcription factor NFAT.
    • Involvement in disease

      Defects in ORAI1 are the cause of immune dysfunction with T-cell inactivation due to calcium entry defect type 1 (IDTICED1) [MIM:612782]. IDTICED1 is an immune disorder characterized by recurrent infections, impaired T-cell activation and proliferative response, decreased T-cell production of cytokines, and normal lymphocytes counts and serum immunoglobulin levels. In surviving patients ectodermal dysplasia with anhydrosis and non-progressive myopathy may be observed.
    • Sequence similarities

      Belongs to the Orai family.
    • Cellular localization

      Cell membrane.
    • Target information above from: UniProt accession Q96D31 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 84876 Human
      • Omim: 610277 Human
      • SwissProt: Q96D31 Human
      • Unigene: 55148 Human
      • Alternative names

        • Calcium release activated calcium channel protein 1 antibody
        • Calcium release activated calcium modulator 1 antibody
        • Calcium release-activated calcium channel protein 1 antibody
        • CRACM 1 antibody
        • CRACM1 antibody
        • CRCM1 antibody
        • CRCM1_HUMAN antibody
        • FLJ14466 antibody
        • Orai 1 antibody
        • ORAI calcium release activated calcium modulator 1 antibody
        • orai1 antibody
        • ORAT1 antibody
        • Protein orai 1 antibody
        • Protein orai-1 antibody
        • TMEM 142A antibody
        • TMEM142A antibody
        • Transmembrane protein 142A antibody
        see all

      Images

      • Western blot - Anti-Orai1 antibody [EPR3394(2)] (ab111960)
        Western blot - Anti-Orai1 antibody [EPR3394(2)] (ab111960)
        All lanes : Anti-Orai1 antibody [EPR3394(2)] (ab111960) at 1/1000 dilution

        Lane 1 : Human platelet lysate
        Lane 2 : Human ovary cancer lysate

        Lysates/proteins at 10 µg per lane.

        Predicted band size: 33 kDa



        The 50kda band is the glycosylated Orai1 protein (PMID: 26956484).

      • Anti-Orai1 antibody [EPR3394(2)] (ab111960)
        Anti-Orai1 antibody [EPR3394(2)] (ab111960)

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (1)

      Publishing research using ab111960? Please let us know so that we can cite the reference in this datasheet.

      ab111960 has been referenced in 1 publication.

      • Zhu M  et al. Store-operated Ca(2+) entry regulates glioma cell migration and invasion via modulation of Pyk2 phosphorylation. J Exp Clin Cancer Res 33:98 (2014). PubMed: 25433371

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-2 of 2 Abreviews or Q&A

      Question

      What is the concentration of lot GR100052?

      Read More

      Abcam community

      Verified customer

      Asked on Jan 23 2013

      Answer

      The concentration of lot GR100052 is 0.509 mg/ml.

      Read More

      Abcam Scientific Support

      Answered on Jan 23 2013

      Question

      I am interested in your product with Cat#: Anti-Orai1 antibody [EPR3394(2)] (ab111960). Can you send me the epitope for this antibody.    Waiting for your reply,  

      Read More

      Abcam community

      Verified customer

      Asked on Sep 14 2012

      Answer

      Thank you for contacting us and your interest in our products. I am pleased to provide the following immunogen information: In order to aid your research we are happy to provide this information to you. However, this immunogen sequence is proprietary (commercially sensitive and confidential). Therefore, please do not share or publish this information. It is strictly for your own use. I hope this information has been of help to you. If you have any further questions, please do not hesitate to contact me.

      Read More

      Abcam Scientific Support

      Answered on Sep 14 2012

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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