Anti-Orai3 antibody (ab224095)
Key features and details
- Rabbit polyclonal to Orai3
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Orai3 antibody
See all Orai3 primary antibodies -
Description
Rabbit polyclonal to Orai3 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human Orai3 aa 179-244.
Sequence:APLDTPTPMVPTSRVPGTLAPVATSLSPASNLPRSSASAAPSQAEPACPP RQACGGGGAHGPGWQA
Database link: Q9BRQ5 -
Positive control
- WB: HEK-293T overexpressing Orai3 cell lysate. IHC-P: Human testis tissue. ICC/IF: A431 cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab224095 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
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| WB | 1/100 - 1/250. Predicted molecular weight: 31 kDa. | |
| IHC-P | 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Key regulator or component of store-operated Ca(2+) channel and transcription factor NFAT nuclear import. -
Sequence similarities
Belongs to the Orai family. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 93129 Human
- Omim: 610930 Human
- SwissProt: Q9BRQ5 Human
- Unigene: 729239 Human
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Alternative names
- MGC13024 antibody
- Orai 3 antibody
- ORAI calcium release activated calcium modulator 3 antibody
see all
Images
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Western blot - Anti-Orai3 antibody (ab224095)All lanes : Anti-Orai3 antibody (ab224095) at 1/100 dilution
Lane 1 : Vector only transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) overexpressing Orai3 (co-expressed with a C-terminal myc-DDK tag), cell lysate
Predicted band size: 31 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Orai3 antibody (ab224095)Paraffin-embedded human testis tissue stained for Orai3 using ab224095 at 1/50 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-Orai3 antibody (ab224095)PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for Orai3 (green) using ab224095 at 4 µg/ml in ICC/IF.
Protocols
Datasheets and documents
References (0)
ab224095 has not yet been referenced specifically in any publications.
