Anti-Orai3 antibody (ab224095)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Orai3 antibody
See all Orai3 primary antibodies -
Description
Rabbit polyclonal to Orai3 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human Orai3 aa 179-244.
Sequence:APLDTPTPMVPTSRVPGTLAPVATSLSPASNLPRSSASAAPSQAEPACPP RQACGGGGAHGPGWQA
Database link: Q9BRQ5 -
Positive control
- WB: HEK-293T overexpressing Orai3 cell lysate. IHC-P: Human testis tissue. ICC/IF: A431 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol, PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab224095 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use a concentration of 1 - 4 µg/ml. Fixation/permeabilization: PFA/Triton X-100. |
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| WB | 1/100 - 1/250. Predicted molecular weight: 31 kDa. | |
| IHC-P | 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Key regulator or component of store-operated Ca(2+) channel and transcription factor NFAT nuclear import. -
Sequence similarities
Belongs to the Orai family. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 93129 Human
- Omim: 610930 Human
- SwissProt: Q9BRQ5 Human
- Unigene: 729239 Human
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Alternative names
- MGC13024 antibody
- Orai 3 antibody
- ORAI calcium release activated calcium modulator 3 antibody
see all
Images
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Western blot - Anti-Orai3 antibody (ab224095)All lanes : Anti-Orai3 antibody (ab224095) at 1/100 dilution
Lane 1 : Vector only transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) overexpressing Orai3 (co-expressed with a C-terminal myc-DDK tag), cell lysate
Predicted band size: 31 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Orai3 antibody (ab224095)Paraffin-embedded human testis tissue stained for Orai3 using ab224095 at 1/50 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-Orai3 antibody (ab224095)PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for Orai3 (green) using ab224095 at 4 µg/ml in ICC/IF.
Protocols
Datasheets and documents
References
ab224095 has not yet been referenced specifically in any publications.
