Orange Mitochondrial Membrane Potential Assay Kit (Flow Cytometry) (ab138898)

Overview

  • Product name

    Orange Mitochondrial Membrane Potential Assay Kit (Flow Cytometry)
    See all Mitochondrial Membrane Potential kits
  • Detection method

    Fluorescent
  • Sample type

    Adherent cells, Suspension cells
  • Assay type

    Direct
  • Product overview

    Orange Mitochondrial Membrane Potential Assay Kit (Flow Cytometry) (ab138898) is designed to detect cell apoptosis by measuring the loss of the mitochondrial membrane potential (MMP). The collapse of mitochondrial membrane potential coincides with the opening of the mitochondrial permeability transition pores, leading to the release of cytochrome C into the cytosol, which in turn triggers other downstream events in the apoptotic cascade.


    ab138898 provides all the essential components with an optimized assay method. This fluorimetric assay uses our proprietary cationic MitoOrange Dye for the detection of apoptosis in cells with the loss of mitochondrial membrane potential. In normal cells, the red fluorescence intensity is increased when MitoOrange Dye is accumulated in the mitochondria. However, in apoptotic cells, the fluorescence intensity of MitoOrange Dye is decreased following the collapse of MMP. Cells stained with MitoOrange Dye can be visualized with a flow cytometer at 488 nm excitation with red emission (FL2 channel). The kit can be used together with other reagents for multi-parametric study of cell vitality and apoptosis. The kit is optimized for screening apoptosis activators and inhibitors with a flow cytometer.

  • Notes

    Related assays

    Review the cell health assay guide to learn about kits to perform a cell viability assaycytotoxicity assay and cell proliferation assay

    Review the metabolism assay guide to learn about assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also about how to assay metabolic function in live cells using your plate reader.

  • Platform

    Fluorescence microscope

Properties

Associated products

Images

  • Flow Cytometric analysis demonstrating the decrease in fluorescence intensity of MitoOrange Dye with the addition of FCCP in Jurkat cells. Jurkat cells were loaded with MitoOrange Dye alone (Blue) or in the presence of 30 µM FCCP (Red) for 15 minutes. The fluorescence intensity of MitoOrange Dye was measured with a flow cytometer using the FL2 channel.

Protocols

References

ab138898 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab138898.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up