Anti-ORC1 antibody [7F6/1] - ChIP Grade (ab60)

Submit a review Q&A (6)References (3)

Overview

  • Product name
    Anti-ORC1 antibody [7F6/1] - ChIP Grade
    See all ORC1 primary antibodies
  • Description
    Mouse monoclonal [7F6/1] to ORC1 - ChIP Grade
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, ChIPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein (Human)

  • Positive control
    • HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab60 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1250 - 1/2500. Detects a band of approximately 120 kDa (predicted molecular weight: 97 kDa).
ChIP Use at an assay dependent dilution.

Target

  • Function
    Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent, however specific DNA sequences that define origins of replication have not been identified so far. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication.
  • Sequence similarities
    Belongs to the ORC1 family.
    Contains 1 BAH domain.
  • Developmental stage
    Expression is cell-cycle regulated, it starts to accumulate in mid-G1 phase, reaches a peak at the G1/S boundary, and decreases to a basal level in S phase (at protein level).
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
    • Alternative names
      • HSORC1 antibody
      • MmORC1 antibody
      • orc1 antibody
      • ORC1_HUMAN antibody
      • ORC1L antibody
      • Origin Recognition Complex 1 antibody
      • Origin recognition complex subunit 1 (yeast homolog) like antibody
      • Origin recognition complex subunit 1 antibody
      • Origin recognition complex subunit 1 homolog antibody
      • Origin recognition complex subunit 1 like (S. cerevisia antibody
      • Origin recognition complex subunit 1 like antibody
      • Origin recognition complex subunit 1 S. cerevisiae homolog like antibody
      • Origin recognition complex, subunit 1 like (yeast) antibody
      • PARC1 antibody
      • Replication control protein 1 antibody
      see all

    References

    This product has been referenced in:
    • Lu F  et al. Regulation of DNA replication and chromosomal polyploidy by the MLL-WDR5-RBBP5 methyltransferases. Biol Open 5:1449-1460 (2016). Read more (PubMed: 27744293) »
    • Beck DB  et al. The role of PR-Set7 in replication licensing depends on Suv4-20h. Genes Dev 26:2580-9 (2012). WB, ChIP ; Human . Read more (PubMed: 23152447) »
    See all 3 Publications for this product

    Publishing research using ab60? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    1-6 of 6 Abreviews or Q&A

    Question

    Thanks for your mail. I have some more questions. I read the reviews and reviews are not good regarding the antibody. I dont understand if the immunogen is a full length human Orc protien which has a mol weight of 97 Kda, why you get a band in place of 120 kd and a shorter band. I wonder is it a cross reacting band and actually no band for endogenous human Orc1 can not be detected with your antibody. Please clarify this point. I am hesitant to use that antibody in my study. Can you please check whether the immunogen was a human Orc1 protein or the yeast Orc1 protein.

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    Answer

    Thank you for your patience, I was away on vacation and was thus unable to reply to you sooner. The immunogen used was Human ORC1 protein. The report of ab60 detecting a 120 kDa band was reported by a customer who used the antibody (see review on the online datasheet) and we don't have any more information at this time regarding the band size detected by this antibody. We make it a policy to post both good and bad reviews on our datasheets such that our customers can make the decision whether or not to go ahead and try the antibody.

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    Question

    Customer would like to know: Datasheet says that by Western blot, this antibody detects a band of 120 kDa, which corresponds to the predicted molecular weight of ORC 1. But customer says that human ORC1 is approx 97-100 kDa. Can this be clarified?

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    Answer

    Thank you for your enquiry. ORC1 from the yeast S. cerevisiae is 120 kDa, and human ORC1 is 97 kDa so the expected band size indicated on the datasheet is referring to yeast. However, a customer's review (please see the review tab on the online datasheet) reported seeing a 120 kDa band in HeLa cells and it seams that the ORC1 protein is quite unstable.

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    Question

    I would like to know the conditions at which this Ab can be detected in normal western blotting analysis (i.e. pH, PBS/ TBS/ tween) thank you

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    Answer

    The antibody should work using normal Western blotting conditions (see general protocol on our website for more information), but it seems that the ORC1 protein is quite unstable (see review on this antibody on the antibody datasheet, top right hand corner).

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    Question

    We already bought 3 vials of the anti-orc1 antibody but none of them is working. Probably the western blotting conditions we are using are not right, so could you please tell us the proper conditions?

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    Answer

    The antibody should work using normal Western blotting conditions (see general protocol on our WEBsite for more information), but it seams that the ORC1 protein is quite unstable (see review on this antibody on the antibody datasheet, top right hand corner).

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    Question

    Is your ORC1 antibody recognize S.cerevisiae Orc1?

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    Answer

    We have no reports of it being tested on S.cerevisae Orc1. If it is closely homologous to mammalian then it may well do.

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    Question

    Does this antibody react with mouse ORC1 on western blots. If it does I would like to buy it.

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    Answer

    Yes, it does react with mouse ORC1

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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