Product nameAnti-ORC2L antibody
DescriptionRabbit polyclonal to ORC2L
Tested applicationsSuitable for: IP, IHC-P, WBmore details
Species reactivityReacts with: Human
Synthetic peptide within Human ORC2L aa 150-200. The exact sequence is proprietary.
Database link: Q13416
- IHC-P: Human breast carcinoma tissue. WB: HeLa and HEK-293T whole cell lysate. IP: HeLa whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab264304 in the following tested applications.
|IP||Use at 2-5 µg/mg of lysate.|
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/2000 - 1/10000.|
RelevanceThe origin recognition complex (ORC) is a highly conserved six subunits protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. The protein encoded by this gene is a subunit of the ORC complex. This protein forms a core complex with ORC3L, -4L, and -5L. It also interacts with CDC45L and MCM10, which are proteins known to be important for the initiation of DNA replication. This protein has been demonstrated to specifically associate with the origin of replication of Epstein Barr virus in human cells, and is thought to be required for DNA replication from viral origin of replication.
- Orc 2 antibody
- ORC 2L antibody
- Orc2 antibody
All lanes : Anti-ORC2L antibody (ab264304) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : HEK-293T whole cell lysate at 50 µg
Developed using the ECL technique.
Exposure time: 3 minutes
ORC2L was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab264304 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab264304 at 0.4 µg/ml.
Lane 1: ab264304 IP in HeLa whole cell lysate.
Lane 2: Control IgG.
Exposure time: 30 secs.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human breast carcinoma tissue labeling ORC2L with ab264304 at 1/200 dilution. Detection: DAB.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab264304 has not yet been referenced specifically in any publications.