Anti-ORC3 antibody - C-terminal (ab179936)
Overview
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Product name
Anti-ORC3 antibody - C-terminal -
Description
Goat polyclonal to ORC3 - C-terminal -
Host species
Goat -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Hamster, Cow, Dog, Pig, Zebrafish
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Immunogen
Synthetic peptide corresponding to Human ORC3 aa 698-711 (C terminal).
Sequence:QKTDHVARLTWGGC
Database link: Q9UBD5 -
Positive control
- HeLa lysate; Human brain cortex tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 99% Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab179936 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 82 kDa. | |
| IHC-P | Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent, however specific DNA sequences that define origins of replication have not been identified so far. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication. -
Sequence similarities
Belongs to the ORC3 family. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Phosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 421822 Chicken
- Entrez Gene: 523714 Cow
- Entrez Gene: 474991 Dog
- Entrez Gene: 100065661 Horse
- Entrez Gene: 23595 Human
- Entrez Gene: 50793 Mouse
- Entrez Gene: 100519524 Pig
- Entrez Gene: 313138 Rat
see all -
Alternative names
- Homolog of latheo Drosophila antibody
- LAT antibody
- LATHEO antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ORC3 antibody - C-terminal (ab179936)Immunohistochemical analysis of formalin-fixed, paraffin embedded Human brain cortex tissue labeling ORC3 with ab179936 at 3.75 µg/ml.
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Western blot - Anti-ORC3 antibody - C-terminal (ab179936)Anti-ORC3 antibody - C-terminal (ab179936) at 2 µg/ml + HeLa lysate
Developed using the ECL technique.
Predicted band size: 82 kDa
Protocols
Datasheets and documents
References
ab179936 has not yet been referenced specifically in any publications.
