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    orc3-antibody-c-terminal-ab179936.pdf

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Epigenetics and Nuclear Signaling DNA / RNA DNA Synthesis Other
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Anti-ORC3 antibody - C-terminal (ab179936)

  • Datasheet
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ORC3 antibody - C-terminal (ab179936)
  • Western blot - Anti-ORC3 antibody - C-terminal (ab179936)

Key features and details

  • Goat polyclonal to ORC3 - C-terminal
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

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Secondary
Product image
Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)

View more associated products

Overview

  • Product name

    Anti-ORC3 antibody - C-terminal
  • Description

    Goat polyclonal to ORC3 - C-terminal
  • Host species

    Goat
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Hamster, Cow, Dog, Pig, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to Human ORC3 aa 698-711 (C terminal).
    Sequence:

    QKTDHVARLTWGGC


    Database link: Q9UBD5
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • HeLa lysate; Human brain cortex tissue.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 0.5% BSA, 99% Tris buffered saline
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Synthesis
    • Other

Associated products

  • Compatible Secondaries

    • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
    • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
  • Isotype control

    • Goat IgG, polyclonal - Isotype Control (ab37373)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab179936 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 82 kDa.
IHC-P
Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 82 kDa.
IHC-P
Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent, however specific DNA sequences that define origins of replication have not been identified so far. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication.
  • Sequence similarities

    Belongs to the ORC3 family.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession Q9UBD5 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 421822 Chicken
    • Entrez Gene: 523714 Cow
    • Entrez Gene: 474991 Dog
    • Entrez Gene: 100065661 Horse
    • Entrez Gene: 23595 Human
    • Entrez Gene: 50793 Mouse
    • Entrez Gene: 100519524 Pig
    • Entrez Gene: 313138 Rat
    • Entrez Gene: 334315 Zebrafish
    • Omim: 604972 Human
    • SwissProt: Q32PJ3 Cow
    • SwissProt: Q9UBD5 Human
    • SwissProt: Q9JK30 Mouse
    • SwissProt: Q4R180 Rat
    • Unigene: 410228 Human
    • Unigene: 399624 Mouse
    • Unigene: 162803 Rat
    see all
  • Alternative names

    • Homolog of latheo Drosophila antibody
    • LAT antibody
    • LATHEO antibody
    • orc3 antibody
    • ORC3_HUMAN antibody
    • ORC3L antibody
    • Origin recognition complex subunit 3 antibody
    • Origin recognition complex subunit 3 honolog antibody
    • Origin recognition complex subunit Latheo antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ORC3 antibody - C-terminal (ab179936)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ORC3 antibody - C-terminal (ab179936)

    Immunohistochemical analysis of formalin-fixed, paraffin embedded Human brain cortex tissue labeling ORC3 with ab179936 at 3.75 µg/ml.

  • Western blot - Anti-ORC3 antibody - C-terminal (ab179936)
    Western blot - Anti-ORC3 antibody - C-terminal (ab179936)
    Anti-ORC3 antibody - C-terminal (ab179936) at 2 µg/ml + HeLa lysate

    Developed using the ECL technique.

    Predicted band size: 82 kDa

Protocols

  • Western blot protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (0)

Publishing research using ab179936? Please let us know so that we can cite the reference in this datasheet.

ab179936 has not yet been referenced specifically in any publications.

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