Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, 5mg/ml Tris, pH 7.3
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab9213 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 82 kDa).Can be blocked with Human ORC3L peptide (ab23155).
Please note that we also detect a band at approximately 35kDa in addition to the predicted band. This could be cell/tissue specific. For example, Thome et al., [J. Biol. Chem, Nov 2000; 275: 35233 - 35241] observed that "brain expressed a 40-45-kDa protein that reacted strongly with the anti-Orc3 antibody". Moreover, splice prediction software (http://www.bioinformatics.ucla.edu/ASAP/) indicated the possibility of a shorter isoform.
FunctionComponent of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent, however specific DNA sequences that define origins of replication have not been identified so far. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication.
Sequence similaritiesBelongs to the ORC3 family.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Phosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- Homolog of Latheo antibody
- homolog of latheo, Drosophila antibody
- LAT antibody
This product has been referenced in:
- Shen Z & Prasanth SG Orc2 protects ORCA from ubiquitin-mediated degradation. Cell Cycle 11:3578-89 (2012). Read more (PubMed: 22935713) »
- Bartke T et al. Nucleosome-interacting proteins regulated by DNA and histone methylation. Cell 143:470-84 (2010). WB ; Human . Read more (PubMed: 21029866) »