Product nameAnti-ORC3L antibody
See all ORC3L primary antibodies
DescriptionRabbit polyclonal to ORC3L
Tested applicationsSuitable for: IHC-P, WBmore details
Unsuitable for: IP
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide, corresponding to a region within the internal sequence amino acids 325-375 of Human ORC3L (NP_036513.2).
- HeLa whole cell lysate and 293T cell lysate
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAffinity purified using an epitope specific to ORC3L immobilized on solid support.
Our Abpromise guarantee covers the use of ab99278 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 82 kDa.|
FunctionComponent of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent, however specific DNA sequences that define origins of replication have not been identified so far. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication.
Sequence similaritiesBelongs to the ORC3 family.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Phosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- Homolog of Latheo antibody
- homolog of latheo, Drosophila antibody
- LAT antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling ORC3L with ab99278 at 1/200 (1µg/mg). Detection: DAB.
All lanes : Anti-ORC3L antibody (ab99278) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 82 kDa
Exposure time: 30 seconds
ab99278 has not yet been referenced specifically in any publications.