Anti-ORC4L antibody (ab9641)
Key features and details
- Goat polyclonal to ORC4L
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
-
Product name
Anti-ORC4L antibody
See all ORC4L primary antibodies -
Description
Goat polyclonal to ORC4L -
Host species
Goat -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow, Dog -
Immunogen
Synthetic peptide corresponding to Human ORC4L aa 400-500 (C terminal). (NP_001177810.1; NP_001177811.1)
Database link: NP_002543.2 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
ChIP Related Products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab9641 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
1 hour primary incubation is recommended for this product. |
Notes |
---|
WB
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa). 1 hour primary incubation is recommended for this product. |
Target
-
Function
Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent, however specific DNA sequences that define origins of replication have not been identified so far. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication. -
Involvement in disease
Defects in ORC4 are the cause of Meier-Gorlin syndrome type 2 (MGORS2) [MIM:613800]. MGORS2 is a syndrome characterized by bilateral microtia, aplasia/hypoplasia of the patellae, and severe intrauterine and postnatal growth retardation with short stature and poor weight gain. Additional clinical findings include anomalies of cranial sutures, microcephaly, apparently low-set and simple ears, microstomia, full lips, highly arched or cleft palate, micrognathia, genitourinary tract anomalies, and various skeletal anomalies. While almost all cases have primordial dwarfism with substantial prenatal and postnatal growth retardation, not all cases have microcephaly, and microtia and absent/hypoplastic patella are absent in some. Despite the presence of microcephaly, intellect is usually normal. -
Sequence similarities
Belongs to the ORC4 family. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 529245 Cow
- Entrez Gene: 476141 Dog
- Entrez Gene: 5000 Human
- Entrez Gene: 26428 Mouse
- Entrez Gene: 295596 Rat
- Omim: 603056 Human
- SwissProt: Q2YDI2 Cow
- SwissProt: O43929 Human
see all -
Alternative names
- Origin recognition complex, subunit 4, S. cerevisiae, homolog of antibody
- FLJ46668 antibody
- HSORC4 antibody
see all
Images
-
Anti-ORC4L antibody (ab9641) at 0.5 µg/ml + Mouse heart lysate at 35 µg
Predicted band size: 50 kDa -
Anti-ORC4L antibody (ab9641) at 0.5 µg/ml + Rat Heart lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 50 kDa
Observed band size: 50 kDa -
ab9641 (2µg/ml) staining of Jurkat lysate (RIPA buffer, 1.4E5 cells per lane). Detected by western blot using chemiluminescence.
-
All lanes : Anti-ORC4L antibody (ab9641) at 1 µg/ml
Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 50 kDa
Observed band size: 49 kDa why is the actual band size different from the predicted?
Additional bands at: 38 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
Datasheets and documents
-
Datasheet download
References (11)
ab9641 has been referenced in 11 publications.
- Hossain M et al. Multiple, short protein binding motifs in ORC1 and CDC6 control the initiation of DNA replication. Mol Cell 81:1951-1969.e6 (2021). PubMed: 33761311
- Cheung MH et al. Human NOC3 is essential for DNA replication licensing in human cells. Cell Cycle 18:605-620 (2019). PubMed: 30741601
- Hossain M & Stillman B Opposing roles for DNA replication initiator proteins ORC1 and CDC6 in control of Cyclin E gene transcription. Elife 5:N/A (2016). IP ; Human . PubMed: 27458800
- Zhang R et al. Targeting GLI by GANT61 involves mechanisms dependent on inhibition of both transcription and DNA licensing. Oncotarget 7:80190-80207 (2016). ICC/IF ; Human . PubMed: 27863397
- Sherstyuk VV et al. Mapping of Replication Origins in the X Inactivation Center of Vole Microtus levis Reveals Extended Replication Initiation Zone. PLoS One 10:e0128497 (2015). WB . PubMed: 26038842