Anti-ORC6 antibody - C-terminal (ab153993)
Key features and details
- Rabbit polyclonal to ORC6 - C-terminal
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ORC6 antibody - C-terminal -
Description
Rabbit polyclonal to ORC6 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide, corresponding to a region within C terminal amino acids 191-252 of Human ORC6 (Uniprot ID: Q9Y5N6).
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Positive control
- 293T, MOLT4 and Raji whole cell lysates; Human TOV-21G xenograft tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab153993 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 28 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 28 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Relevance
The origin recognition complex (ORC) is a highly conserved six subunit protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. ORC6L is a subunit of the ORC complex. It has been shown that ORC6L and and ORC1L are loosely associated with the core complex consisting of ORC2L, 3L, 4L and 5L. Gene silencing studies with small interfering RNA demonstrated that ORC6L plays an essential role in coordinating chromosome replication and segregation with cytokinesis. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 23594 Human
- Omim: 607213 Human
- SwissProt: Q9Y5N6 Human
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Alternative names
- ORC6L antibody
- origin recognition complex subunit 6 antibody
- origin recognition complex subunit 6 homolog like (yeast) antibody
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab153993 has been referenced in 1 publication.
- Chen H et al. ORC6, Negatively Regulated by miR-1-3p, Promotes Proliferation, Migration, and Invasion of Hepatocellular Carcinoma Cells. Front Cell Dev Biol 9:652292 (2021). PubMed: 34395415