Anti-Orexin A antibody (ab6214)
Key features and details
- Rabbit polyclonal to Orexin A
- Suitable for: ELISA, IHC-Fr, IHC-FoFr, IHC-P
- Reacts with: Mouse, Rat, Cow
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Orexin A antibody
See all Orexin A primary antibodies -
Description
Rabbit polyclonal to Orexin A -
Host species
Rabbit -
Specificity
Immunohistochemical analysis of rat brain indicates a good specificity for this serum. -
Tested applications
Suitable for: ELISA, IHC-Fr, IHC-FoFr, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Cow -
Immunogen
Synthetic peptide corresponding to Cow Orexin A aa 1-100 conjugated to keyhole limpet haemocyanin (Glutaraldehyde).
Database link: P56717 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7 -
Concentration information loading...
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Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab6214 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA |
Use at an assay dependent concentration.
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IHC-Fr |
Use at an assay dependent concentration.
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IHC-FoFr |
Use at an assay dependent concentration. PubMed: 16052497
Following perfusion fixation with 4% PFA, cryoprotect the tissue in 20% sucrose overnight. Cut on cryostat sections for free floating IHC. Incubate primary antibody for 48 hrs at 4 degree C. |
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IHC-P | (2) |
Use at an assay dependent concentration.
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Notes |
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ELISA
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
IHC-FoFr
Use at an assay dependent concentration. PubMed: 16052497 Following perfusion fixation with 4% PFA, cryoprotect the tissue in 20% sucrose overnight. Cut on cryostat sections for free floating IHC. Incubate primary antibody for 48 hrs at 4 degree C. |
IHC-P
Use at an assay dependent concentration. |
Target
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Relevance
Orexin A (hypocretin 1) a 33 amino acid peptide and orexin B (hypocretin 2), a 28 amino acid peptide, are both derived from a common 130 amino acid precursor, prepro orexin. Orexin A and Orexin B stimulate food consumption when administered intracerebroventricularly to rats. Orexin gene expression in the brain is highly restricted to distinct populations of neurons located in specific hypothalamic regions, including the lateral hypothalamic area (LHA), a region implicated in feeding behaviour. Orexin A and orexin B bind to and activate two closely related G protein coupled receptors (GPCRs), termed Orexin 1 (OX1) and Orexin 2 (OX2) receptors. Dysfunction of the orexin peptide system has been linked to narcolepsy. -
Cellular localization
Rough endoplasmic reticulum. Cytoplasmic vesicle. Cell junction, synapse. Note: Associated with perikaryal rough endoplasmic reticulum as well as cytoplasmic large granular vesicles at synapses. -
Database links
- Entrez Gene: 15171 Mouse
- Entrez Gene: 25723 Rat
- SwissProt: O55241 Mouse
- SwissProt: O55232 Rat
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Alternative names
- HCRT antibody
- HCRT1 antibody
- HCRT2 antibody
see all
Images
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Immunohistochemical staining of orexin A in rat lateral hypothalamus useing Anti-Orexin A antibody (ab6214) at 1:50000 dilution. The primary antibody was detected with the biotinylated secondary antibody-ABC method and nickel-diaminobenzidine chromogen.
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Immunohistochemical staining of orexin A in rat zona incerta neurons using Rabbit antibody to Orexin A (ab6214) at a dilution of 1 in 1000.
Datasheets and documents
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Datasheet download
References (11)
ab6214 has been referenced in 11 publications.
- Guo S et al. Specific Silencing of Microglial Gene Expression in the Rat Brain by Nanoparticle-Based Small Interfering RNA Delivery. ACS Appl Mater Interfaces 14:5066-5079 (2022). PubMed: 35041392
- Zhao P et al. Orexin A peptidergic system: comparative sleep behavior, morphology and population in brains between wild type and Alzheimer's disease mice. Brain Struct Funct 227:1051-1065 (2022). PubMed: 35066609
- Yaghmaeian Salmani B et al. Selective requirement for polycomb repressor complex 2 in the generation of specific hypothalamic neuronal subtypes. Development 149:N/A (2022). PubMed: 35245348
- Parekh RU et al. Hypothalamic kinin B1 receptor mediates orexin system hyperactivity in neurogenic hypertension. Sci Rep 11:21050 (2021). PubMed: 34702886
- Zhang B et al. Hypothalamic orexin and mechanistic target of rapamycin activation mediate sleep dysfunction in a mouse model of tuberous sclerosis complex. Neurobiol Dis 134:104615 (2020). PubMed: 31605778