Product nameAnti-Orexin A antibody
See all Orexin A primary antibodies
DescriptionRabbit polyclonal to Orexin A
SpecificityImmunohistochemical analysis of rat brain indicates a good specificity for this serum.
Tested applicationsSuitable for: IHC-FoFr, ELISA, IHC-Fr, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Cow
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Our Abpromise guarantee covers the use of ab6214 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-FoFr||1/1000. PubMed: 16052497
Following perfusion fixation with 4% PFA, cryoprotect the tissue in 20% sucrose overnight. Cut on cryostat sections for free floating IHC. Incubate primary antibody for 48 hrs at 4°C.
|IHC-Fr||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.|
RelevanceOrexin A (hypocretin 1) a 33 amino acid peptide and orexin B (hypocretin 2), a 28 amino acid peptide, are both derived from a common 130 amino acid precursor, prepro orexin. Orexin A and Orexin B stimulate food consumption when administered intracerebroventricularly to rats. Orexin gene expression in the brain is highly restricted to distinct populations of neurons located in specific hypothalamic regions, including the lateral hypothalamic area (LHA), a region implicated in feeding behaviour. Orexin A and orexin B bind to and activate two closely related G protein coupled receptors (GPCRs), termed Orexin 1 (OX1) and Orexin 2 (OX2) receptors. Dysfunction of the orexin peptide system has been linked to narcolepsy.
Cellular localizationRough endoplasmic reticulum. Cytoplasmic vesicle. Cell junction, synapse. Note: Associated with perikaryal rough endoplasmic reticulum as well as cytoplasmic large granular vesicles at synapses.
- HCRT antibody
- HCRT1 antibody
- HCRT2 antibody
ab6214 staining Pan Orexin in mouse brain tissue sections by Immunohistochemistry (PFA fixed free floating sections). Tissue samples were fixed with paraformaldehyde, permeablized with 1% Triton X-100 and blocked with 1% BSA for 10 minutes at room temperature. The sample was incubated with primary antibody (1/2000 in 1X PBS + 0.1% Triton X-100 + 1% BSA) at 4°C for 24 hours. An Alexa Fluor® 594-conjugated donkey anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.
Immunohistochemical staining of orexin A in rat lateral hypothalamus useing Anti-Orexin A antibody (ab6214) at 1:50000 dilution. The primary antibody was detected with the biotinylated secondary antibody-ABC method and nickel-diaminobenzidine chromogen.
Immunohistochemical staining of orexin A in rat zona incerta neurons using Rabbit antibody to Orexin A (ab6214) at a dilution of 1 in 1000.
This product has been referenced in:
- Verma D et al. Pancreatic polypeptide and its central Y4 receptors are essential for cued fear extinction and permanent suppression of fear. Br J Pharmacol 173:1925-38 (2016). IHC . Read more (PubMed: 26844810) »
- Kerman IA et al. Distinct populations of presympathetic-premotor neurons express orexin or melanin-concentrating hormone in the rat lateral hypothalamus. J Comp Neurol 505:586-601 (2007). Read more (PubMed: 17924541) »