Anti-Orexin antibody (ab199275)
Key features and details
- Rabbit polyclonal to Orexin
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Orexin antibody -
Description
Rabbit polyclonal to Orexin -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide within Human Orexin aa 2-15 (N terminal). The exact sequence is proprietary. Synthetic peptide corresponding to residues near the N terminal of human hypocretin (orexin) neuropeptide precursor. NP_001515.
Sequence:NLPSTKVSWAAVTL
Database link: O43612 -
Positive control
- Human lung cancer tissue
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab199275 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/20 - 1/100. |
Target
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Function
Neuropeptides that play a significant role in the regulation of food intake and sleep-wakefulness, possibly by coordinating the complex behavioral and physiologic responses of these complementary homeostatic functions. A broader role in the homeostatic regulation of energy metabolism, autonomic function, hormonal balance and the regulation of body fluids, is also suggested. Orexin-A binds to both OX1R and OX2R with a high affinity, whereas orexin-B binds only to OX2R with a similar high affinity. -
Tissue specificity
Abundantly expressed in subthalamic nucleus but undetectable in other brain regions tested (hypothalamus was not tested) and in heart, placenta, lung, liver, skeletal muscle, kidney and pancreas. -
Involvement in disease
Defects in HCRT are the cause of narcolepsy type 1 (NRCLP1) [MIM:161400]. Narcolepsy is a neurological disabling sleep disorder, characterized by excessive daytime sleepiness, sleep fragmentation, symptoms of abnormal rapid-eye-movement (REM) sleep, such as cataplexy, hypnagogic hallucinations, and sleep paralysis. Cataplexy is a sudden loss of muscle tone triggered by emotions, which is the most valuable clinical feature used to diagnose narcolepsy. Human narcolepsy is primarily a sporadically occurring disorder but familial clustering has been observed. Note=Human narcolepsy is associated with a deficient orexin system. Orexins are absent and/or greatly diminished in the brain and cerebrospinal fluid (CSF) of most narcoleptic patients. -
Sequence similarities
Belongs to the orexin family. -
Post-translational
modificationsSpecific enzymatic cleavages at paired basic residues yield the different active peptides. -
Cellular localization
Rough endoplasmic reticulum. Cytoplasmic vesicle. Cell junction > synapse. Associated with perikaryal rough endoplasmic reticulum as well as cytoplasmic large granular vesicles at synapses. - Information by UniProt
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Database links
- Entrez Gene: 3060 Human
- Entrez Gene: 15171 Mouse
- Entrez Gene: 25723 Rat
- Omim: 602358 Human
- SwissProt: O43612 Human
- SwissProt: O55241 Mouse
- SwissProt: O55232 Rat
- Unigene: 158348 Human
see all -
Alternative names
- Hcrt antibody
- Hcrt1 antibody
- Hcrt2 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Orexin antibody (ab199275)
Immunohistochemical analysis of paraffin embedded Human lung cancer tissue labeling Pan Orexin with ab199275 at 1/20 dilution.
Datasheets and documents
References (0)
ab199275 has not yet been referenced specifically in any publications.
