Anti-ORF7a Protein antibody [3C9] (ab273435)
Key features and details
- Mouse monoclonal [3C9] to ORF7a Protein - Coronavirus
- Suitable for: WB, ICC/IF
- Isotype: IgG1
Overview
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Product name
Anti-ORF7a Protein antibody [3C9] -
Description
Mouse monoclonal [3C9] to ORF7a Protein - Coronavirus -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: SARS-CoV-2 -
Immunogen
Recombinant fragment corresponding to Human coronavirus ORF7a Protein aa 1-150.
Database link: P59635 -
Positive control
- WB: Caco2 and Vero E6 whole cell lysate infected with SARS-CoV-2 Protein 7a. ICC/IF: Vero E6 whole cells expressing SARS-CoV-2 Protein 7a.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
3C9 -
Isotype
IgG1 -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab273435 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000.
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ICC/IF |
Use at an assay dependent concentration.
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Notes |
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WB
1/500 - 1/3000. |
ICC/IF
Use at an assay dependent concentration. |
Target
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Alternative names
- Accessory protein 7a antibody
- Protein U122 antibody
- Protein X4 antibody
Images
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Anti-ORF7a Protein antibody [3C9] (ab273435) at 1/1000 dilution + Recombinant SARS-CoV2 ORF7a Fc chimera protein cell lysate at 0.5 µg
Performed under reducing conditions.
Observed band size: 40 kDa why is the actual band size different from the predicted?False colour image of Western blot: Anti-ORF7a Protein antibody [3C9] - Coronavirus staining at 1/1000 dilution, shown in green; Rabbit anti-alpha Tubulin antibody [EP1332Y] (ab52866) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab273435 was shown to bind specifically to ORF7a Protein. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) at 1/20000 dilution.
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All lanes : Anti-ORF7a Protein antibody [3C9] (ab273435) at 1/1000 dilution
Lane 1 : Mock, Caco2 (human colorectal adenocarcinoma cell line) whole cell lysate
Lane 2 : SARS-CoV infected, Caco2 whole cell lysate
Lane 3 : SARS-CoV-2, Caco2 whole cell lysate -
Immunofluorescence analysis of Vero E6 (african green monkey kidney epithelial cell line) cells labelling Protein 7a (green) using ab273435. Red: MitoTracker. Left Panel is merged images.
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All lanes : Anti-ORF7a Protein antibody [3C9] (ab273435) at 1/500 dilution
Lane 1 : SARS-CoV-infected Vero E6 (african green monkey kidney epithelial cell line) whole cell lysate
Lane 2 : 8 hours after SARS-CoV-infected Vero E6 whole cell lysate
Lane 3 : 16 hours after SARS-CoV-infected Vero E6 whole cell lysate
Lane 4 : 24 hours after SARS-CoV-infected Vero E6 whole cell lysate
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab273435 has not yet been referenced specifically in any publications.