Overview

  • Product name

  • Description

    Rabbit polyclonal to ORMDL3
  • Host species

    Rabbit
  • Specificity

    ab107639 may cross-react with ORMDL1 and ORMDL2.
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human ORMDL3 aa 53-81 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: Q8N138

  • Positive control

    • WB: Mouse testis tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab107639 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 17 kDa.

Target

  • Function

    Negative regulator of sphingolipid synthesis. May indirectly regulate endoplasmic reticulum-mediated Ca(+2) signaling.
  • Tissue specificity

    Widely expressed. Expressed in adult and fetal heart, brain, lung, liver, skeletal muscle and kidney. Expressed in adult pancreas and placenta and in fetal spleen abd thymus.
  • Involvement in disease

    Genetic variations in ORMDL3 are associated with susceptibility to asthma (ASTHMA) [MIM:600807]. The most common chronic disease affecting children and young adults. It is a complex genetic disorder with a heterogeneous phenotype, largely attributed to the interactions among many genes and between these genes and the environment. It is characterized by recurrent attacks of paroxysmal dyspnea, with weezing due to spasmodic contraction of the bronchi.
  • Sequence similarities

    Belongs to the ORM family.
  • Cellular localization

    Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • ORM1 (S. cerevisiae)-like 3 antibody
    • ORM1-like 3 (S. cerevisiae) antibody
    • ORM1-like 3 antibody
    • ORM1-like protein 3 antibody
    • ORMDL sphingolipid biosynthesis regulator 3 antibody
    • Ormdl3 antibody
    • ORML3_HUMAN antibody
    • OTTHUMP00000164334 antibody
    see all

Images

  • All lanes : Anti-ORMDL3 antibody (ab107639) at 1/1000 dilution

    Lane 1 : Jurkat whole cell lysate
    Lane 2 : Mouse testis lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution

    Predicted band size: 17 kDa



    Blocking buffer 5% NFDM/TBST
    Diluting buffer  5% NFDM/TBST

References

This product has been referenced in:

  • Yang R  et al. Investigating the regulatory role of ORMDL3 in airway barrier dysfunction using in vivo and in vitro models. Int J Mol Med 44:535-548 (2019). Read more (PubMed: 31173170) »
  • Wu XL  et al. Methylation status of ORMDL3 regulates cytokine production and p-ERK/MMP9 pathway expression. Exp Cell Res 372:43-51 (2018). Read more (PubMed: 30217493) »
See all 4 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Yes, product Anti-ORMDL3 antibody (ab107639) is able to detect endogenous ORMDL3 protein in lysate samples, without overexpression.

Western Blot data showing use of ab107639 for the detection of endogenous ORMDL3 in Jurkat whole cell lysates and mouse testis lysates is available on the product webpage and datasheet.

Product ab107639 is covered by our Abpromise Guarantee for use in WB with both human and mouse samples.

Read More

Answer

Yes - product Anti-ORMDL1 + ORMDL2 + ORMDL3 antibody (ab128660) is a pan-specific ORMDL family antibody. It will detect ORMDL1, ORMDL2 and ORMDL3.

Ab128660 is able to detect endogenous ORMDL1-3 in human cell lysates by WB; reactivity against other sample species is also predicted.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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