Overview

  • Product name

  • Description

    Rabbit polyclonal to ORMDL3
  • Host species

    Rabbit
  • Specificity

    The immunogenic peptide shares 100% homology with family members ORMDL1 and ORMDL2. Cross reactivity with these family members has not been tested.
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human ORMDL3 aa 53-81 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Sequence:

    NLIHNMGMYIFLHTVKGTPFETPDQGKAR


    Database link: Q8N138

  • Positive control

    • Mouse testis whole cell lysate, Human spleen tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab211522 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/1000. Predicted molecular weight: 18 kDa.

Target

  • Function

    Negative regulator of sphingolipid synthesis. May indirectly regulate endoplasmic reticulum-mediated Ca(+2) signaling.
  • Tissue specificity

    Widely expressed. Expressed in adult and fetal heart, brain, lung, liver, skeletal muscle and kidney. Expressed in adult pancreas and placenta and in fetal spleen abd thymus.
  • Involvement in disease

    Genetic variations in ORMDL3 are associated with susceptibility to asthma (ASTHMA) [MIM:600807]. The most common chronic disease affecting children and young adults. It is a complex genetic disorder with a heterogeneous phenotype, largely attributed to the interactions among many genes and between these genes and the environment. It is characterized by recurrent attacks of paroxysmal dyspnea, with weezing due to spasmodic contraction of the bronchi.
  • Sequence similarities

    Belongs to the ORM family.
  • Cellular localization

    Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • ORM1 (S. cerevisiae)-like 3 antibody
    • ORM1-like 3 (S. cerevisiae) antibody
    • ORM1-like 3 antibody
    • ORM1-like protein 3 antibody
    • ORMDL sphingolipid biosynthesis regulator 3 antibody
    • Ormdl3 antibody
    • ORML3_HUMAN antibody
    • OTTHUMP00000164334 antibody
    see all

Images

  • Anti-ORMDL3 antibody (ab211522) at 1/1000 dilution + Mouse testis whole cell lysate at 35 µg

    Predicted band size: 18 kDa

  • Immunohistochemical analysis of Human spleen tissue labeling ORMDL3 with ab211522 at a 1/100 dilution. Heat mediated antigen retrieval used.

References

This product has been referenced in:

  • Li Z  et al. Targeting the miR-665-3p-ATG4B-autophagy axis relieves inflammation and apoptosis in intestinal ischemia/reperfusion. Cell Death Dis 9:483 (2018). Read more (PubMed: 29706629) »
See 1 Publication for this product

Customer reviews and Q&As

Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (mouse whole lung lysate)
Gel Running Conditions
Reduced Denaturing (4-20%)
Loading amount
50 µg
Specification
mouse whole lung lysate
Blocking step
Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Aug 19 2016

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