Anti-ornithine aminotransferase antibody (ab231808)
Key features and details
- Rabbit polyclonal to ornithine aminotransferase
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human, Pig
- Isotype: IgG
Overview
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Product name
Anti-ornithine aminotransferase antibody
See all ornithine aminotransferase primary antibodies -
Description
Rabbit polyclonal to ornithine aminotransferase -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Rat, Human, Pig
Predicted to work with: Mouse, Cow -
Immunogen
Recombinant full length protein (His-T7-tag) corresponding to Human ornithine aminotransferase aa 1 to the C-terminus. (Expressed in E.coli).
Database link: P04181-2 -
Positive control
- WB: Recombinant human ornithine aminotransferase protein; Pig liver and kidney lysates; Rat testis and pancreas lysates. IHC-P: Human lung cancer, colorectal cancer, kidney, glioma and liver tissues.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab231808 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab231808 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 33, 49 kDa.
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IHC-P |
Use a concentration of 5 - 20 µg/ml.
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Notes |
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WB
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 33, 49 kDa. |
IHC-P
Use a concentration of 5 - 20 µg/ml. |
Target
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Relevance
Ornithine aminotransferase (OAT) is a key mitochondrial enzyme in the pathway that converts arginine and ornithine into the major excitatory and inhibitory neurotransmitters glutamate and GABA. Mutations that result in a deficiency of this enzyme cause the autosomal recessive eye disease Gyrate Atrophy. OAT has also been linked with prostate cancer and alternatively activated macrophages -
Cellular localization
Mitochondrion matrix. -
Database links
- Entrez Gene: 505323 Cow
- Entrez Gene: 4942 Human
- Entrez Gene: 18242 Mouse
- Entrez Gene: 64313 Rat
- Omim: 613349 Human
- SwissProt: Q3ZCF5 Cow
- SwissProt: P04181 Human
- SwissProt: P29758 Mouse
see all -
Alternative names
- GACR antibody
- Gyrate atrophy antibody
- HOGA antibody
see all
Images
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Paraffin-embedded human lung cancer tissue stained for ornithine aminotransferase using ab231808 at 30 μg/ml in immunohistochemical analysis.
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Paraffin-embedded human colorectal cancer tissue stained for ornithine aminotransferase using ab231808 at 20 μg/ml in immunohistochemical analysis.
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Paraffin-embedded human kidney tissue stained for ornithine aminotransferase using ab231808 at 20 μg/ml in immunohistochemical analysis.
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Paraffin-embedded human glioma tissue stained for ornithine aminotransferase using ab231808 at 20 μg/ml in immunohistochemical analysis.
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Paraffin-embedded human liver tissue stained for ornithine aminotransferase using ab231808 at 20 μg/ml in immunohistochemical analysis.
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Anti-ornithine aminotransferase antibody (ab231808) at 1 µg/ml + Recombinant human ornithine aminotransferase protein
Secondary
HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 33, 49 kDa -
All lanes : Anti-ornithine aminotransferase antibody (ab231808) at 1 µg/ml
Lane 1 : Pig liver lysate
Lane 2 : Pig kidney lysate
Lane 3 : Rat testis lysate
Lane 4 : Rat pancreas lysate
Secondary
All lanes : HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 33, 49 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab231808 has not yet been referenced specifically in any publications.