Key features and details
- Rabbit polyclonal to ornithine aminotransferase
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human, Pig
- Isotype: IgG
Product nameAnti-ornithine aminotransferase antibody
See all ornithine aminotransferase primary antibodies
DescriptionRabbit polyclonal to ornithine aminotransferase
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Rat, Human, Pig
Predicted to work with: Mouse, Cow
Recombinant full length protein (His-T7-tag) corresponding to Human ornithine aminotransferase aa 1-301. (Expressed in E.coli).
MNTGVEAGETACKLARKWGYTVKGIQKYKAKIVFAAGNFWGRTLSAISSS TDPTSYDGFGPFMPGFDIIPYNDLPALERALQDPNVAAFMVEPIQGEAGV VVPDPGYLMGVRELCTRHQVLFIADEIQTGLARTGRWLAVDYENVRPDIV LLGKALSGGLYPVSAVLCDDDIMLTIKPGEHGSTYGGNPLGCRVAIAALE VLEEENLAENADKLGIILRNELMKLPSDVVTAVRGKGLLNAIVIKETKDW DAWKVCLRLRDNGLLAKPTHGDIIRFAPPLVIKEDELRESIEIINKTILS F
Database link: P04181-2
- WB: Recombinant human ornithine aminotransferase protein; Pig liver and kidney lysates; Rat testis and pancreas lysates. IHC-P: Human lung cancer, colorectal cancer, kidney, glioma and liver tissues.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab231808 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab231808 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 33, 49 kDa.|
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
RelevanceOrnithine aminotransferase (OAT) is a key mitochondrial enzyme in the pathway that converts arginine and ornithine into the major excitatory and inhibitory neurotransmitters glutamate and GABA. Mutations that result in a deficiency of this enzyme cause the autosomal recessive eye disease Gyrate Atrophy. OAT has also been linked with prostate cancer and alternatively activated macrophages
Cellular localizationMitochondrion matrix.
- GACR antibody
- Gyrate atrophy antibody
- HOGA antibody
Paraffin-embedded human lung cancer tissue stained for ornithine aminotransferase using ab231808 at 30 μg/ml in immunohistochemical analysis.
Paraffin-embedded human colorectal cancer tissue stained for ornithine aminotransferase using ab231808 at 20 μg/ml in immunohistochemical analysis.
Paraffin-embedded human kidney tissue stained for ornithine aminotransferase using ab231808 at 20 μg/ml in immunohistochemical analysis.
Paraffin-embedded human glioma tissue stained for ornithine aminotransferase using ab231808 at 20 μg/ml in immunohistochemical analysis.
Paraffin-embedded human liver tissue stained for ornithine aminotransferase using ab231808 at 20 μg/ml in immunohistochemical analysis.
Anti-ornithine aminotransferase antibody (ab231808) at 1 µg/ml + Recombinant human ornithine aminotransferase protein
HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 33, 49 kDa
All lanes : Anti-ornithine aminotransferase antibody (ab231808) at 1 µg/ml
Lane 1 : Pig liver lysate
Lane 2 : Pig kidney lysate
Lane 3 : Rat testis lysate
Lane 4 : Rat pancreas lysate
All lanes : HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 33, 49 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab231808 has not yet been referenced specifically in any publications.