Overview

  • Product name

    Anti-Ornithine Carbamoyltransferase/OTC antibody
    See all Ornithine Carbamoyltransferase/OTC primary antibodies
  • Description

    Rabbit polyclonal to Ornithine Carbamoyltransferase/OTC
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, Flow Cyt, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human Ornithine Carbamoyltransferase/OTC aa 71-98 (internal sequence) conjugated to keyhole limpet haemocyanin.
    Database link: 5009

  • Positive control

    • HepG2 cell line lysate
  • General notes

    Previously labelled as Ornithine Carbamoyltransferase. 

Properties

Applications

Our Abpromise guarantee covers the use of ab91418 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/10 - 1/50.
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

WB 1/1000. Predicted molecular weight: 40 kDa.

Target

  • Tissue specificity

    Mainly expressed in liver and intestinal mucosa.
  • Pathway

    Nitrogen metabolism; urea cycle; L-citrulline from L-ornithine and carbamoyl phosphate: step 1/1.
  • Involvement in disease

    Ornithine carbamoyltransferase deficiency
  • Sequence similarities

    Belongs to the ATCase/OTCase family.
  • Post-translational
    modifications

    Acetylation at Lys-88 negatively regulates ornithine carbamoyltransferase activity in response to nutrient signals.
  • Cellular localization

    Mitochondrion matrix.
  • Information by UniProt
  • Database links

  • Alternative names

    • EC 2.1.3.3 antibody
    • MGC129967 antibody
    • MGC129968 antibody
    • MGC138856 antibody
    • OCTD antibody
    • Ornithine Carbamoyltransferase antibody
    • Ornithine carbamoyltransferase mitochondrial antibody
    • Ornithine carbamoyltransferase, mitochondrial antibody
    • Ornithine transcarbamylase antibody
    • Otc antibody
    • OTC_HUMAN antibody
    • OTCase antibody
    see all

Images

  • Anti-Ornithine Carbamoyltransferase/OTC antibody (ab91418) at 1/100 dilution + HepG2 cell line lysate at 35 µg

    Predicted band size: 40 kDa

  • All lanes : Anti-Ornithine Carbamoyltransferase/OTC antibody (ab91418)

    Lane 1 : SW620 cell lysate
    Lane 2 : Mouse liver tissue lysate
    Lane 3 : Rat liver tissue lysate

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 40 kDa

  • Immunohistochemical staining of formalin-fixed and paraffin-embedded human hepatoarcinoma with ab91418, followed by HRP-conjugated secondary antibody and DAB staining.

  • Flow cytometric analysis of ATDC5 cells using ab91418 (green histogram) compared to a negative control cell (blue histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

References

This product has been referenced in:

  • Alexandrou C  et al. Sensitivity of Colorectal Cancer to Arginine Deprivation Therapy is Shaped by Differential Expression of Urea Cycle Enzymes. Sci Rep 8:12096 (2018). Read more (PubMed: 30108309) »
See 1 Publication for this product

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